Pluripotent marker expression and differentiation of human second trimester Mesenchymal Stem Cells

Gonzalez, Rafael; Maki, Chad B.; Pacchiarotti, Jason; Csontos, S.; Pham, Jennifer; Slepko, Natalia; Patel, Amit N.; Silva, F.

doi:10.1016/j.bbrc.2007.08.033

Cited by 31 publications

(32 citation statements)

References 26 publications

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“…Researchers have shown that stromal cells can be reliably harvested from both sources [9,20], and because these are fetus-derived tissues, they represent a potential form of autologous stromal cell therapy for patients undergoing fetal surgery. We have chosen to focus on placenta-derived tissues as they can be harvested via chorionic villous sampling, the procedure of choice at a gestational age of 12 weeks (when MMC is typically diagnosed), with minimal risk to the mother or fetus, and can be expanded in culture for autologous MMC repair during the second trimester [21].…”

Section: Discussionmentioning

confidence: 99%

“…Cells harvested from placental tissue have been characterized as MSCs [9], but have also been shown to display more unique properties. Lee et al [10] compared MSCs derived from placenta with standard bone marrow- and adipose tissue-derived stromal cells and found that placenta-derived MSCs express higher levels of immunomodulatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

“…Given the known anti-inflammatory and epithelialization-promoting properties of the fetal membranes [3,4,5,6,7,8], we continued to believe that there was promise for FM repair for MMC. Recent literature has shown that the placenta is also a source of cells that have remarkable immunomodulatory properties, can improve wound healing, and exhibit neuroprotective effects [9,10,11,12,13]. …”

Section: Introductionmentioning

confidence: 99%

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Age Does Matter: A Pilot Comparison of Placenta-Derived Stromal Cells for in utero Repair of Myelomeningocele Using a Lamb Model

Brown¹,

Keller²,

Lankford³

et al. 2015

Fetal Diagn Ther

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Introduction: Fetal amniotic membranes (FM) have been shown to preserve spinal cord histology in the fetal sheep model of myelomeningocele (MMC). This study compares the effectiveness of placenta-derived mesenchymal stromal cells (PMSCs) from early-gestation versus term-gestation placenta to augment FM repair to improve distal motor function in a sheep model. Methods: Fetal lambs (n = 4) underwent surgical MMC creation followed by repair with FM patch with term-gestation PMSCs (n = 1), FM with early-gestation PMSCs (n = 1), FM only (n = 1), and skin closure only (n = 1). Histopathology and motor assessment was performed. Results: Histopathologic analysis demonstrated increased preservation of spinal cord architecture and large neurons in the lamb repaired with early-gestation cells compared to all others. Lambs repaired with skin closure only, FM alone, and term-gestation PMSCs exhibited extremely limited distal motor function; the lamb repaired with early-gestation PMSCs was capable of normal ambulation. Discussion: This pilot study is the first in vivo comparison of different gestational-age placenta-derived stromal cells for repair in the fetal sheep MMC model. The preservation of large neurons and markedly improved motor function in the lamb repaired with early-gestation cells suggest that early-gestation placental stromal cells may exhibit unique properties that augment in utero MMC repair to improve paralysis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Age Does Matter: A Pilot Comparison of Placenta-Derived Stromal Cells for in utero Repair of Myelomeningocele Using a Lamb Model

Brown¹,

Keller²,

Lankford³

et al. 2015

Fetal Diagn Ther

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show abstract

“…In addition, it has been demonstrated in vitro that MSCs are able to differentiate into mesodermal tissues such as osteoblasts, chondroblasts, adipocytes, and skeletal myoblasts. 76,77 MSCs have the ability to migrate and target specific tissues. This nesting property (homing) by chemotaxis is an event that allows cells to migrate from a remote area in the body to find a damaged organ or tissue in a specific site.…”

Section: Stem Cellsmentioning

confidence: 99%

“…79 Both in vitro and in vivo models have demonstrated the plasticity of MSCs, giving rise to non-hematopoietic cells such as myocytes, tenocytes, and nerve cells. 64,[74][75][76][77]79 The MSCs used in patients modulate immune responses, such as in collagen diseases, multiple sclerosis, bone marrow transplantation, or graft versus host disease (GVHD).…”

Section: Stem Cellsmentioning

confidence: 99%

Stem Cells Targeting Inflammation as Potential Anti-aging Strategies and Therapies

Gonzalez¹,

Woynarowski²,

Geffner³

2015

CTTT

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Human aging is associated with a vast array of clinical disorders that all relate to the body's inability to maintain homeostasis. In our bodies, the healing process requires tight control of the acute inflammatory response. When the levels of inflammatory markers such as interleukin-6, tumor necrosis factor alpha, and C-reactive protein are elevated, there is a direct correlation with several chronic diseases of aging such as cardiovascular disease, cognitive decline, and physical disability. Extensive studies have shown strong evidence that elevated levels of these pro-inflammatory mediators may predict disease and disability in the aging population. As there is no cure for aging, a key question is how to modulate the effects of inflammation on aging and how to maintain healthy aging. Stem cells offer alternative approaches for treating various diseases/disorders. The use of stem cells as immune system modulators has shown tremendous promise. Mesenchymal stem cells (MSCs) have specific immunomodulatory properties that may help control inflammation. Several animal studies demonstrate that intravenous infusions of MSCs can decrease the pro-inflammatory response while increasing the anti-inflammatory response. Moreover, human clinical studies using MSCs in autoimmune and other inflammatory diseases have demonstrated modulation of the inflammatory response. Therefore, the use of stem cells as anti-aging therapies may offer many an alternative method of slowing down or deterring several of the detrimental aspects of aging.

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Fetal Tissue Engineering: Regenerative Capacity of Fetal Stem Cells

Moschidou

Fisk

Fundamentals of Tissue Engineering and Regenerative Medicine

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Pluripotent marker expression and differentiation of human second trimester Mesenchymal Stem Cells

Cited by 31 publications

References 26 publications

Age Does Matter: A Pilot Comparison of Placenta-Derived Stromal Cells for in utero Repair of Myelomeningocele Using a Lamb Model

Age Does Matter: A Pilot Comparison of Placenta-Derived Stromal Cells for in utero Repair of Myelomeningocele Using a Lamb Model

Stem Cells Targeting Inflammation as Potential Anti-aging Strategies and Therapies

Fetal Tissue Engineering: Regenerative Capacity of Fetal Stem Cells

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