Pluripotent Core in Bovine Embryos: A Review

Águila, Luis; Osycka‐Salut, Claudia; Treulén, Favián; Felmer, Ricardo

doi:10.20944/preprints202203.0003.v1

Cited by 2 publications

(1 citation statement)

References 79 publications

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“…Apesar disso, o comportamento dos genes durante a maturação oocitária e desenvolvimento pré-implantacional corroboram os resultados encontrados na literatura acerca de ambos os genes (KHAN et al, 2012;AGUILA et al, 2022). De maneira semelhante ao OCT4, o silenciamento do gene NANOG em zigotos bovinos não interfere no desenvolvimento embrionário e formação da blastocele, fato este que leva a crer que ele não tem influência na formação da MCI (ORTEGA et al, 2019).…”

Section: Discussão | 82unclassified

Domínios ICRs 1 e 2 de bovinos no período pré-implantação: expressão, perfil da metilação do DNA e suas associações com a reprogramação epigenética

Verruma¹

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Section: Discussão | 82unclassified

Domínios ICRs 1 e 2 de bovinos no período pré-implantação: expressão, perfil da metilação do DNA e suas associações com a reprogramação epigenética

Verruma¹

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Combined Exogenous Activation of Bovine Oocytes: Effects on Maturation-Promoting Factor, Mitogen-Activated Protein Kinases, and Embryonic Competence

Valencia,

Pérez-García,

Aguila

et al. 2023

IJMS

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Oocyte activation via dual inhibition of protein synthesis and phosphorylation has improved in vitro embryo production in different mammalian species. In this study, we evaluated the effects of the combination of cycloheximide (CHX), dimethyl amino purine (DMAP), and anisomycin (ANY) on the activation of bovine oocytes, particularly on dynamics of MPF and MAPKs, embryonic developmental potential, and quality. The results showed that the cleavage and blastocyst rates, as well as levels of CCNB1, CDK1, p-CDK1Thr161, and p-CDK1Thr14-Tyr15, were similar among groups; ANY and ANY + CHX reduced the expression of ERK1/2 compared to DMAP-combinations (p < 0.05), whereas ANY + DMAP, CHX + DMAP, and ANY + CHX + DMAP reduced p-ERK1/2 compared to ANY and ANY + CHX treatments (p < 0.05). The quality of blastocysts in terms of cell counts, their allocation, and the numbers of TUNEL-positive cells did not differ among groups. However, transcript levels of POU5F1 were higher in embryos derived from ANY + CHX + DMAP treatment compared to other groups, while expression levels of CDX2 did not show differences. In addition, the BCL2A1/BAX ratio of the ANY + CHX + DMAP treatment was significantly low compared to the ANY treatment (p < 0.05) and did not differ significantly from the other treatments. In conclusion, oocyte activation by dual inhibition of protein synthesis and phosphorylation induces MPF inactivation without degradation of CCNB1, while MAPK inactivation occurs differentially between these inhibitors. Thus, although the combined use of these inhibitors does not affect early developmental competence in vitro, it positively impacts the expression of transcripts associated with embryonic quality.

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Pluripotent Core in Bovine Embryos: A Review

Cited by 2 publications

References 79 publications

Domínios ICRs 1 e 2 de bovinos no período pré-implantação: expressão, perfil da metilação do DNA e suas associações com a reprogramação epigenética

Domínios ICRs 1 e 2 de bovinos no período pré-implantação: expressão, perfil da metilação do DNA e suas associações com a reprogramação epigenética

Combined Exogenous Activation of Bovine Oocytes: Effects on Maturation-Promoting Factor, Mitogen-Activated Protein Kinases, and Embryonic Competence

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