Pluripotent and Mesenchymal Stem Cells—Challenging Sources for Derivation of Myoblast

Archacka, Karolina; Brzóska, Edyta; Ciemerych, Maria A.; Czerwińska, Areta M.; Grabowska, Iwona; Kowalski, Kamil; Zimowska, Małgorzata

doi:10.1007/978-3-319-70685-6_6

Cited by 8 publications

(10 citation statements)

References 237 publications

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“…In addition, iPSCs could be produced from patient somatic cells what would allow the derivation of syngeneic cells for transplantation. Unfortunately, the methods of pluripotent stem cells differentiation are still not perfect, and some of the cell types, such as skeletal muscle myoblasts, are not easy to derive [37,78]. Moreover, there are also well-founded worries that if not properly prepared these cells can differentiate chaotically within the recipient tissue, forming so-called teratomas, what might pose serious health risk [79].…”

Section: Discussionmentioning

confidence: 99%

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Adipose Tissue-Derived Stromal Cells in Matrigel Impact the Regeneration of Severely Damaged Skeletal Muscles

Grabowska

Zimowska

Maciejewska

et al. 2019

IJMS

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In case of large injuries of skeletal muscles the pool of endogenous stem cells, i.e., satellite cells, might be not sufficient to secure proper regeneration. Such failure in reconstruction is often associated with loss of muscle mass and excessive formation of connective tissue. Therapies aiming to improve skeletal muscle regeneration and prevent fibrosis may rely on the transplantation of different types of stem cell. Among such cells are adipose tissue-derived stromal cells (ADSCs) which are relatively easy to isolate, culture, and manipulate. Our study aimed to verify applicability of ADSCs in the therapies of severely injured skeletal muscles. We tested whether 3D structures obtained from Matrigel populated with ADSCs and transplanted to regenerating mouse gastrocnemius muscles could improve the regeneration. In addition, ADSCs used in this study were pretreated with myoblasts-conditioned medium or anti-TGFβ antibody, i.e., the factors modifying their ability to proliferate, migrate, or differentiate. Analyses performed one week after injury allowed us to show the impact of 3D cultured control and pretreated ADSCs at muscle mass and structure, as well as fibrosis development immune response of the injured muscle.

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Section: Discussionmentioning

confidence: 99%

“…In vivo, these cells were also shown to be able to incorporate into regenerating injured [33,34] or dystrophic muscles [35,36]. Unfortunately, proportion of myofibers formed with the participation of BM-MSCs was very low, thus, such transplantation did not present sufficient therapeutic potential [37].…”

Section: Introductionmentioning

confidence: 99%

Adipose Tissue-Derived Stromal Cells in Matrigel Impact the Regeneration of Severely Damaged Skeletal Muscles

Grabowska

Zimowska

Maciejewska

et al. 2019

IJMS

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“…One of the studied cells are bone marrow-derived stromal cells, also known as bone marrow-derived “mesenchymal” stem/stromal cells (bone marrow-derived MSCs). However, it should be noted that these cells do not present naïve myogenic potential [ 30 ]. Bone marrow-derived MSCs are a heterogeneous population [ 31 ], typically isolated from bone marrow on the basis of their ability to adhere to the culture plate’s surface.…”

Section: Introductionmentioning

confidence: 99%

Hypoxia preconditioned bone marrow-derived mesenchymal stromal/stem cells enhance myoblast fusion and skeletal muscle regeneration

et al. 2021

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Background The skeletal muscle reconstruction occurs thanks to unipotent stem cells, i.e., satellite cells. The satellite cells remain quiescent and localized between myofiber sarcolemma and basal lamina. They are activated in response to muscle injury, proliferate, differentiate into myoblasts, and recreate myofibers. The stem and progenitor cells support skeletal muscle regeneration, which could be disturbed by extensive damage, sarcopenia, cachexia, or genetic diseases like dystrophy. Many lines of evidence showed that the level of oxygen regulates the course of cell proliferation and differentiation. Methods In the present study, we analyzed hypoxia impact on human and pig bone marrow-derived mesenchymal stromal cell (MSC) and mouse myoblast proliferation, differentiation, and fusion. Moreover, the influence of the transplantation of human bone marrow-derived MSCs cultured under hypoxic conditions on skeletal muscle regeneration was studied. Results We showed that bone marrow-derived MSCs increased VEGF expression and improved myogenesis under hypoxic conditions in vitro. Transplantation of hypoxia preconditioned bone marrow-derived MSCs into injured muscles resulted in the improved cell engraftment and formation of new vessels. Conclusions We suggested that SDF-1 and VEGF secreted by hypoxia preconditioned bone marrow-derived MSCs played an essential role in cell engraftment and angiogenesis. Importantly, hypoxia preconditioned bone marrow-derived MSCs more efficiently engrafted injured muscles; however, they did not undergo myogenic differentiation.

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“…Among the tissues in which development and function are extensively analyzed using PSCs are skeletal muscles. The protocols allowing derivation of myogenic progenitors from PSCs cover various approaches involving overexpression of selected factors, such as PAX3 or PAX7 (e.g., [ 1 , 2 , 3 ]), using DNA demethylating agents, such as 5-azacitidine (5azaC) (e.g., [ 4 , 5 , 6 ]), as well as culture under precisely defined conditions (e.g., [ 7 , 8 ], for review see [ 9 ]).…”

Section: Introductionmentioning

confidence: 99%

PAX7 Balances the Cell Cycle Progression via Regulating Expression of Dnmt3b and Apobec2 in Differentiating PSCs

Florkowska

Meszka

Nowacka

et al. 2021

Cells

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PAX7 transcription factor plays a crucial role in embryonic myogenesis and in adult muscles in which it secures proper function of satellite cells, including regulation of their self renewal. PAX7 downregulation is necessary for the myogenic differentiation of satellite cells induced after muscle damage, what is prerequisite step for regeneration. Using differentiating pluripotent stem cells we documented that the absence of functional PAX7 facilitates proliferation. Such action is executed by the modulation of the expression of two proteins involved in the DNA methylation, i.e., Dnmt3b and Apobec2. Increase in Dnmt3b expression led to the downregulation of the CDK inhibitors and facilitated cell cycle progression. Changes in Apobec2 expression, on the other hand, differently impacted proliferation/differentiation balance, depending on the experimental model used.

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Pluripotent and Mesenchymal Stem Cells—Challenging Sources for Derivation of Myoblast

Cited by 8 publications

References 237 publications

Adipose Tissue-Derived Stromal Cells in Matrigel Impact the Regeneration of Severely Damaged Skeletal Muscles

Adipose Tissue-Derived Stromal Cells in Matrigel Impact the Regeneration of Severely Damaged Skeletal Muscles

Hypoxia preconditioned bone marrow-derived mesenchymal stromal/stem cells enhance myoblast fusion and skeletal muscle regeneration

PAX7 Balances the Cell Cycle Progression via Regulating Expression of Dnmt3b and Apobec2 in Differentiating PSCs

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