Pluripotent and committed haemopoietic progenitor cells in rat peripheral blood

Ivanović, Zoran; Petakov, M; Jovčić, Gordana; Biljanović-Paunović, L; Balint, Bela; Milenković, P

doi:10.1007/bf01320992

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…Later, these findings were confirmed in baboons 3 and humans 4 . Apart from the stem cells with the capacity of hematopoietic reconstitution in vivo, steady‐state peripheral blood contains the committed 5‐7 and pluripotent 8‐10 progenitors.…”

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confidence: 99%

Whole‐blood leukodepletion filters as a source of CD34+ progenitors potentially usable in cell therapy

et al. 2005

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mentioning

confidence: 99%

Whole‐blood leukodepletion filters as a source of CD34+ progenitors potentially usable in cell therapy

et al. 2005

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“…Naime, CD34 je ime koje je dato transmembranskom glikoproteinu, prisutnom na površini MĆH, ali i nekih stromalnih ćelija. Adultne ćelije koje eksprimuju CD34 antigena, a koje su poreklom iz kostne srži ili periferne krvi, omogućavaju kompletnu i dugotrajnu repopulaciju kostne srži primaoca (nakon prihvatanja kalema), sa sledstvenom rekonstitucijom hematopoeze [16][17][18][19][20][21].…”

Section: Grafting In Clinical Practiceunclassified

“…given to a transmembrane glycoprotein present on the surface of HSCs and some stromal cells. Adult cells expressing the CD34 antigen, obtained from BM or peripheral blood (PB), stimulate complete and long-term repopulation of the recipient's bone marrow (following engraftment), with subsequent hematopoietic reconstitution [16][17][18][19][20][21].…”

Section: Grafting In Clinical Practicementioning

confidence: 99%

“…Namely, these stromal cells stimulate SCs by producing specific growth factors, such as the Flt3-ligand, c-kit-ligand or SC-factor, as well as interleukins, granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor (GM-CSF and G-CSF). In addition to secreting cytokines, stromal cells regulate the adhesion of SC molecules and allow them to stay in the BM or migrate to the area where the respective cell type is needed [14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

“…granulocyte colony-stimulating factor -G-CSF). Uz lučenje citokina, stromalne ćelije regulišu i adheziju molekula MĆ i omogućavaju im da ostanu u kostnoj srži ili da migriraju u ono područje gde je dati tip ćelije potreban [14][15][16][17][18][19].…”

Section: Introductionunclassified

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A stem cell overview: From evolving hemobiological concepts to (auto)grafting in clinical practice

Balint¹,

Pavlović²,

Marković³

et al. 2022

Srpski medicinski časopis Lekarske komore

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Conventional hematopoietic stem cell transplantation is a well-known treatment method for numerous acquired and congenital hematopoietic disorders, disorders of the immune system, as well as certain metabolic disorders. Stem cells (SCs) can be defined as cells capable of self-renewal with a high proliferative capacity and the potential to differentiate into functionally competent mature cells. Stem cells can be divided into embryonic SCs (ESCs) and tissue-specific or adult SCs - such as bone marrow (BM) stem cells, peripheral blood (PB) stem cells, and SCs derived from umbilical cord blood (UCB), as well as other non-hematopoietic or somatic SCs. SCs in adults are characteristically considered to be restricted in their regenerative and differentiative potential, while embryonic stem cells are 'true' totipotent/pluripotent cells, due to their ability to develop into endoderm, ectoderm, or mesoderm - all three embryonic tissue types in the human body. They are the most promising, but also the most controversial type of potentially transplantable SCs. Immature hematopoietic SCs have the potential of differentiating, not only into all blood cells, but also into some somatic cell types (SC plasticity). In different clinical settings, the transplantation of immature stem cells leads to the repopulation of recipient bone marrow, with subsequent complete, stable, and long-term reconstitution of hematopoiesis. Given that immature stem cells are also capable of homing to different tissues, autologous stem cell implantation into a damaged and/or ischemic area induces their colonizing and consecutive transdifferentiating into cell lineages of the host organ, including neovascularization. Thus, they are clinically applicable in the field of regenerative medicine for the treatment of myocardial, brain, vascular, liver, pancreatic, and other tissue damage. The purpose of this overview is to recapitulate the key developments in the rapidly evolving area of stem cell research, as well as to review the use of SCs in conventional transplantations and in regenerative medicine. Additionally, a brief critical evaluation of our own stem cell research will be summarized.

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Hemopoietic stem cell proliferation in Belgrade rats: to complete the parable

Ivanović

1997

Hematol Cell Ther

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The Belgrade laboratory (b/b) rat is a mutant animal suffering of a severe anemia, resulting from a severe intracellular iron deficiency. This deficiency is determined by an impaired intracellular transport of iron due to a block located between the endocytic vesicles and the cytosolic iron transport to mitochondria. This results in a complex series of disturbances affecting hemopoietic stem and progenitor cells, as well as growth factor production. The gene affected by the "b" mutation is unknown. This review summarizes the current knowledge on b/b rat hemopoiesis, with the focus on pluripotent hemopoietic progenitors, such as Spleen Colony Forming Units scored at day 8 after transplantation (CFU-Sd8) and more primitive stem cells (pre-CFU-S) responsible for Marrow Repopulating Ability. A special effort was made to describe the effects of b/b mutation on hemopoietic stem cells distinguishing between the alterations connected to the primary defect (intracellular iron deficiency), and those secondary to severe anemia (severe chronic hypoxia). The intracellular iron deficiency is responsible for a reversible cytostatic effect on CFU-Sd8, while the overcoming of this proliferative block reveals that the size of bone marrow CFU-Sd8 population is limited by the level of oxygenation. The size of pre-CFU-S population is also limited by the level of oxygenation, implying that, in contrast to the normal situation, in b/b rats pre-CFU-S are actively proliferating and therefore sensitive to hypoxia. This review also explains how to get more reliable data on the effects of intracellular iron deficiency and hypoxia on the proliferation of hemopoietic stem and progenitor cells and points to the potential importance of b/b rat phenomenon for fundamental research in cell biology and in the search of new ways to restrain the proliferation of malignant cells.

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Pluripotent and committed haemopoietic progenitor cells in rat peripheral blood

Cited by 6 publications

References 20 publications

Whole‐blood leukodepletion filters as a source of CD34+ progenitors potentially usable in cell therapy

Whole‐blood leukodepletion filters as a source of CD34+ progenitors potentially usable in cell therapy

A stem cell overview: From evolving hemobiological concepts to (auto)grafting in clinical practice

Hemopoietic stem cell proliferation in Belgrade rats: to complete the parable

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