Pluripotency of mesenchymal cells derived from synovial fluid in patients with temporomandibular joint disorder

Abstract

These cells may play a role in the regenerative response during arthritic diseases and are promising candidates for developing novel cell-based therapeutic approaches for postnatal skeletal tissue repair.

Comparison of human mesenchymal stem cells derived from bone marrow, synovial fluid, adult dental pulp, and exfoliated deciduous tooth pulp

“…Synovial fluid was collected from patients with TMJ disorders and the SFCs were isolated as described previously. 9 Pulp tissues were excised from normal third molars extracted for impaction and from normally exfoliated deciduous teeth. The tissues were minced, digested for 1 h at 37 8C with 0.3% type I collagenase and 0.4% dispase, and passed through a 70-mm filter to yield single-cell suspensions.…”

“…PCR amplification of the resulting cDNAs for GAPDH, PPARG2, LPL, TUBB3, and MAP2 was performed under the following conditions: 35 cycles at 94 8C for 45 s, 64 8C for 45 s, and 72 8C for 60 s. After PCR, 10 ml of each reaction was subjected to 2% agarose gel electrophoresis followed by ethidium bromide staining. [8][9][10] Alkaline phosphatase (ALP) activity For osteogenic differentiation on day 28 and for chondrogenic differentiation on day 14, the induction medium was removed and washed with PBS, and the cells were harvested with lysis buffer (pH 7.4) that consisted of 150 mM NaCl, 3 mM NaHCO 3 , 0.1% Triton X-100, and distilled water, and homogenized. The ALP activity in the supernatant obtained was determined using the p-nitrophenylphosphate method (LabAssay ALP; Wako), and the protein concentration was determined using the Micro BCA Protein Assay Reagent Kit (Pierce, Thermo Fisher Scientific, Rockford, IL, USA).…”

“…These are derived from very accessible tissue sources and are capable of providing sufficient cells for potential clinical application. 8,9 In the present study, distinctive populations of pluripotent cells were isolated from bone marrow, synovial fluid, adult dental pulp, and exfoliated deciduous tooth pulp and their potential for multipotentiality compared. Their ability to undergo osteogenic, chondrogenic, adipogenic, and neurogenic differentiation was also assessed.…”

<b>Estudo <i>in vitro</i> do potencial de diferenciação condrogênico e osteogênico de células mesenquimais obtidas de líquido e membrana sinovial de equinos</b>

“…As células-tronco mesenquimais podem ser isoladas de uma variedade de tecidos como medula óssea, tecido adiposo, líquido amniótico, cordão umbilical, líquido sinovial e membrana sinovial (DE BARI et al, 2001;KOGA et al, 2007;JONES et al, 2008;NIMURA et al, 2008;ZHANG et al, 2008;KIM et al, 2011;KOYAMA et al, 2011;LEE et al, 2012a;SEKIYA et al, 2012). A fascinante descoberta de que o tecido sinovial é uma fonte de células-tronco mesenquimais, as caracterizaram como grande promessa para tratamento de enfermidades de origem osteoarticulares.…”

“…Isto se deve, em parte, por se tratarem de células com habilidades comparáveis às outras fontes e essencialmente por seu alto potencial condrogênico. Estas características as distinguem das demais e sustentam a hipótese de que são principais candidatas para a regeneração da cartilagem ARUFE et al, 2009;JONES, 2011;KRAWETZ et al, 2012) A fração celular presente tanto no líquido sinovial (LS), quanto na membrana sinovial (MS) são conhecidas por sinoviócitos do tipo A (MAPP; REVELL, 1987), do tipo B (KITAMURA et al, 1999(KITAMURA et al, , 2001) e recentemente, foram encontradas células-tronco mesenquimais, que são facilmente expandidas em cultura celular, um método simples que promove a proliferação e concentração mais abundante das células, favorecendo o estudo in vitro e conduzindo para futura utilização clínica (KOYAMA et al, 2011).…”