Pluripotency of Male Germline Stem Cells

Kim, Sung-Tae; Belmonte, Juan Carlos Izpisúa

doi:10.1007/s10059-011-1024-4

Cited by 6 publications

(5 citation statements)

References 101 publications

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“…Particularly, in vitro utilization of germline cells that possess greater developmental potential can offer a novel way of deriving superior animals through grafting this in vitro system onto existing genetic breeding and transgenic technology (Gong et al, 2008;Shin et al, 2008;Kim et al, 2009;Kim and Izpisua Belmonte, 2011). In fish, however, a limited number of studies has been conducted to establish in vitro systems using germline cells, and most of those have been restricted to small fish models (Hong et al, 2004;Kawasaki et al, 2012;Nóbrega et al, 2010;.…”

Section: Introductionmentioning

confidence: 98%

Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli

et al. 2016

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This study was conducted to identify the general conditions for the isolation and in vitro culture of ovary-derived cells in rockfish (Sebastes schlegeli). The effects of three different enzymes on cell retrieval from ovarian tissues were evaluated first, and then the ovary-dissociated cells were cultured under various culture conditions, with varying basal media and culture temperatures, addition of growth factors, and/or culture types. We found that collagenase type I treatment was effective for cell isolation from ovarian tissues. From a total of 42 trials to evaluate the effects of basal media and culture temperatures on cell culture of ovary-dissociated cells, we observed that Leibovitz's L15 medium was more supportive than Dulbecco's modified Eagle's medium for culture, and the cells could grow at all three temperatures tested, 15, 20, and 25°C, at least up to passage 2. However, growth factor addition did not improve cell growth. Introduction of suspension culture after monolayer culture expanded the culture period significantly more than did monolayer culture alone. Our results may provide a basis for developing an in vitro system for S. schlegeli germline cell culture, which will ultimately lead to improvement of the species.

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Section: Introductionmentioning

confidence: 98%

Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli

et al. 2016

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“…It should be noted that high differentiation capacity of the mammalian maGSCs has been demonstrated by several studies [32][33][34]. In fact, these stem cells, derived from adult mouse testis (GSCs), could show the characteristics ESC-pluripotency including the expression of the PSCs specific transcription factors and differentiation into three embryonic germ layers including heart lineages and functional CMCs [18,35,36]. Similarly, having an equal ESC property in the human GSCs has been reported by Meyer, et al in 2010 [37].…”

Section: Stem Cellsmentioning

confidence: 99%

Application of Stem Cell Technologies to Regenerate Injured Myocardium and Improve Cardiac Function

Mardanpour

Nayernia²,

Khodayari

et al. 2019

Cell Physiol Biochem

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In the recent decades, cardiovascular diseases emerged as the major leading cause of human mortality. However, current clinical approaches still do not encompass a thorough therapeutic solution for improving heart function of the patients who suffered an extensive myocardial injury. Based on this status quo, stem cells could become a novel option, as a natural source of the new myocardium lineage cells, being capable of paracrine factors secretion, protection or even regeneration of the damaged heart muscle. Efficient stem cell-based therapy of the heart should lead to repair or/and replacement of the degenerated tissue with functional myocardial and endothelial cells. Hereon, various types of pluripotent and multipotent stem cells have been already studied in the pre-clinical and clinical settings, demonstrating their cardiomyogenic and regenerative potential. In this context, as a type of male adult stem/ progenitors, spermatogonial stem cells feature a remarkable ability for a formation of cardiovascular lineages, based on our own observations. Presented data supports the presumption, that spermatogonial stem cells not only have a suitable capacity to generate functional heart cells but can also potentially improve the function of an injured myocardium. In this review article, we first describe the essential molecular and pathophysiological mechanisms involved in the heart tissue injury. Afterwards, based on our ongoing study, we review the impact of the stem cell technologies on the regeneration therapy in cardiovascular and myocardial diseases. Particular emphasis is being put on the usability of spermatogonial stem cells in cardiac therapy. Review

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“…Due to ethical barrier to generate normal embryo mediated hES, the genetically defected non-usable embryo has been used to generate hES for academic purpose [ 7 ]. Furthermore, aborted fetus derived human embryonic germ cell (hEG) were pursued as alternatives [ 8 ].…”

Section: Ipsc As Pluripotent Stem Cellmentioning

confidence: 99%

Disease modeling and cell based therapy with iPSC: future therapeutic option with fast and safe application

Kim

2014

Blood Res

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Induced pluripotent stem cell (iPSC) technology has shown us great hope to treat various human diseases which have been known as untreatable and further endows personalized medicine for future therapy without ethical issues and immunological rejection which embryonic stem cell (hES) treatment has faced. It has been agreed that iPSCs knowledge can be harnessed from disease modeling which mimics human pathological development rather than trials utilizing conventional rodent and cell lines. Now, we can routinely generate iPSC from patient specific cell sources, such as skin fibroblast, hair follicle cells, patient blood samples and even urine containing small amount of epithelial cells. iPSC has both similarity and dissimilarity to hES. iPSC is similar enough to regenerate tissue and even full organism as ES does, however what we want for therapeutic advantage is limited to regenerated tissue and lineage specific differentiation. Depending on the lineage and type of cells, both tissue memory containing (DNA rearrangement/epigenetics) and non-containing iPSC can be generated. This makes iPSC even better choice to perform disease modeling as well as cell based therapy. Tissue memory containing iPSC from mature leukocytes would be beneficial for curing cancer and infectious disease. In this review, the benefit of iPSC for translational approaches will be presented.

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Pluripotency of Male Germline Stem Cells

Cited by 6 publications

References 101 publications

Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli

Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli

Application of Stem Cell Technologies to Regenerate Injured Myocardium and Improve Cardiac Function

Disease modeling and cell based therapy with iPSC: future therapeutic option with fast and safe application

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