Pluripotency in avian species

Pain, Bertrand; Kress, Clémence; Rival‐Gervier, Sylvie

doi:10.1387/ijdb.170322bp

Cited by 5 publications

(6 citation statements)

References 133 publications

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“…Chicken embryonic stem cells (cESC) are propagated in a pluripotent ‘undifferentiated’ state on inactivated (by means of irradiation) feeder cells (mouse embryonic fibroblast cells (STO)) and exhibit morphology similar to mouse ESC [28]. The culture conditions that support the undifferentiated phenotype are well-documented and include the use of growth factors and cytokines such as mouse LIF, and a combination of human IL-6/SCF/IGF-1/IL-6Ra in the medium [9]. The undifferentiated status of cESC was confirmed by flow cytometry for the pluripotency marker, surface antigen SSEA1.…”

Section: Resultsmentioning

confidence: 99%

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Chicken Embryonic-Stem Cells Are Permissive to Poxvirus Recombinant Vaccine Vectors

Giotis

Montillet

Pain

et al. 2019

Genes

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The discovery of mammalian pluripotent embryonic stem cells (ESC) has revolutionised cell research and regenerative medicine. More recently discovered chicken ESC (cESC), though less intensively studied, are increasingly popular as vaccine substrates due to a dearth of avian cell lines. Information on the comparative performance of cESC with common vaccine viruses is limited. Using RNA-sequencing, we compared cESC transcriptional programmes elicited by stimulation with chicken type I interferon or infection with vaccine viruses routinely propagated in primary chicken embryo fibroblasts (CEF). We used poxviruses (fowlpox virus (FWPV) FP9, canarypox virus (CNPV), and modified vaccinia virus Ankara (MVA)) and a birnavirus (infectious bursal disease virus (IBDV) PBG98). Interferon-stimulated genes (ISGs) were induced in cESC to levels comparable to those in CEF and immortalised chicken fibroblast DF-1 cells. cESC are permissive (with distinct host transcriptional responses) to MVA, FP9, and CNPV but, surprisingly, not to PBG98. MVA, CNPV, and FP9 suppressed innate immune responses, while PBG98 induced a subset of ISGs. Dysregulation of signalling pathways (i.e., NFκB, TRAF) was observed, which might affect immune responses and viral replication. In conclusion, we show that cESC are an attractive alternative substrate to study and propagate poxvirus recombinant vaccine vectors.

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Section: Resultsmentioning

confidence: 99%

“…They are positive for alkaline phosphatase and the SSEA1 antigen, and can differentiate into various cell types in vitro or when injected into recipient embryos [7,8]. cESC can be grown and maintained relatively easy in culture and can sustain their phenotypic characteristics after extensive expansion [7,9].…”

Section: Introductionmentioning

confidence: 99%

Chicken Embryonic-Stem Cells Are Permissive to Poxvirus Recombinant Vaccine Vectors

Giotis

Montillet

Pain

et al. 2019

Genes

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“…Despite the diversity of cell types in the testis, most testicular cells can be derived from iPSCs, including PGCs or spermatogonial stem cells, which can self-renew and differentiate into the testicular organoid. The purification of these cells have revolutionised this field [50][51][52].…”

Section: Current Models Of Testicular Organoidsmentioning

confidence: 99%

Progress and challenges in developing organoids in farm animal species for the study of reproduction and their applications to reproductive biotechnologies

Bourdon

Cadoret

Charpigny

et al. 2021

Vet Res

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Within the past decades, major progress has been accomplished in isolating germ/stem/pluripotent cells, in refining culture medium and conditions and in establishing 3-dimensional culture systems, towards developing organoids for organs involved in reproduction in mice and to some extent in humans. Haploid male germ cells were generated in vitro from primordial germ cells. So were oocytes, with additional support from ovarian cells and subsequent follicle culture. Going on with the female reproductive tract, spherical oviduct organoids were obtained from adult stem/progenitor cells. Multicellular endometrial structures mimicking functional uterine glands were derived from endometrial cells. Trophoblastic stem cells were induced to form 3-dimensional syncytial-like structures and exhibited invasive properties, a crucial point for placentation. Finally, considering the embryo itself, pluripotent embryonic cells together with additional extra-embryonic cells, could self-organize into a blastoid, and eventually into a post-implantation-like embryo. Most of these accomplishments have yet to be reached in farm animals, but much effort is devoted towards this goal. Here, we review the progress and discuss the specific challenges of developing organoids for the study of reproductive biology in these species. We consider the use of such organoids in basic research to delineate the physiological mechanisms involved at each step of the reproductive process, or to understand how they are altered by environmental factors relevant to animal breeding. We evaluate their potential in reproduction of animals with a high genetic value, from a breeding point of view or in the context of preserving local breeds with limited headcounts.

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“…ESCs and iPSCs from agriculturally relevant ungulate species, such as pigs and cows, have recently been successfully derived and characterized [35][36][37][38] , while the derivation of bona fide ESCs or iPSCs from avian species, namely chicken, remains elusive. Established culture conditions can support stable long-term culture of pluripotent cells derived from the avian blastoderm, and attempts to derive avian iPSCs have resulted in partially reprogrammed cell lines 39 .…”

Section: Cell Selectionmentioning

confidence: 99%

Scientific, sustainability and regulatory challenges of cultured meat

et al. 2020

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Cellular agriculture is an emerging branch of biotechnology that aims to address issues associated with the environmental impact, animal welfare and sustainability challenges of conventional animal farming for meat production. Cultured meat can be produced by applying current cell culture practices and biomanufacturing methods and utilizing mammalian cell lines and cell and gene therapy products to generate tissue or nutritional proteins for human consumption. However, significant improvements and modifications are needed for the process to be cost efficient and robust enough to be brought to production at scale for food supply. Here, we review the scientific and social challenges in transforming cultured meat into a viable commercial option, covering aspects from cell selection and medium optimization to biomaterials, tissue engineering, regulation and consumer acceptance.

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Pluripotency in avian species

Cited by 5 publications

References 133 publications

Chicken Embryonic-Stem Cells Are Permissive to Poxvirus Recombinant Vaccine Vectors

Chicken Embryonic-Stem Cells Are Permissive to Poxvirus Recombinant Vaccine Vectors

Progress and challenges in developing organoids in farm animal species for the study of reproduction and their applications to reproductive biotechnologies

Scientific, sustainability and regulatory challenges of cultured meat

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