Pluripotency-Associated microRNAs in Early Vertebrate Embryos and Stem Cells

Maraghechi, Pouneh; Aponte, Maria Teresa Salinas; Ecker, András; Lázár, Bence; Tóth, Roland; Szabadi, Nikolett Tokodyné; Gócza, Elen

doi:10.3390/genes14071434

Cited by 2 publications

(1 citation statement)

References 155 publications

(241 reference statements)

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“…During the late stages of pregnancy, syncytiotrophoblast (STB)-EVs are released directly into the maternal circulation and can be uniquely recognized by a placental alkaline phosphatase [ 83 ]. STB-EVs have been shown to carry several proteins including endoglin, plasminogen activator inhibitor, soluble fms-like kinase (sFlt), and endothelial nitric oxide synthase [ 84 , 85 ], as well as miRNAs [ 86 , 87 ], tRNA [ 88 ], and DNA [ 81 , 89 ]. The physiological functions of peripherally circulating STB-EVs in both in vitro and in vivo investigations are broad and their molecular contents as well as surface markers are powerful and cannot be underestimated.…”

Section: Biology Of Extracellular Vesiclesmentioning

confidence: 99%

Unraveling the Multifaceted Roles of Extracellular Vesicles: Insights into Biology, Pharmacology, and Pharmaceutical Applications for Drug Delivery

Al-Jipouri,

Eritja,

Bozic

2023

IJMS

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Extracellular vesicles (EVs) are nanoparticles released from various cell types that have emerged as powerful new therapeutic option for a variety of diseases. EVs are involved in the transmission of biological signals between cells and in the regulation of a variety of biological processes, highlighting them as potential novel targets/platforms for therapeutics intervention and/or delivery. Therefore, it is necessary to investigate new aspects of EVs’ biogenesis, biodistribution, metabolism, and excretion as well as safety/compatibility of both unmodified and engineered EVs upon administration in different pharmaceutical dosage forms and delivery systems. In this review, we summarize the current knowledge of essential physiological and pathological roles of EVs in different organs and organ systems. We provide an overview regarding application of EVs as therapeutic targets, therapeutics, and drug delivery platforms. We also explore various approaches implemented over the years to improve the dosage of specific EV products for different administration routes.

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Section: Biology Of Extracellular Vesiclesmentioning

confidence: 99%

Unraveling the Multifaceted Roles of Extracellular Vesicles: Insights into Biology, Pharmacology, and Pharmaceutical Applications for Drug Delivery

Al-Jipouri,

Eritja,

Bozic

2023

IJMS

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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”

Rodríguez-Varela,

Vautier,

Mucci

et al. 2024

Preprint

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Human pluripotent stem cells (hPSCs), which include embryonic and induced pluripotent stem cells (hESCs and hiPSCs, respectively), have an unusual cell cycle structure which consists of a short G1 phase and the absence of the G1/S checkpoint regulation. E2F transcription factors (E2Fs) play an important role in the G1/S transition. G1 duration contributes to hPSC fate determination, and microRNAs (miRNAs) play critical roles in this commitment. As little is known about the interplay between E2Fs and miRNAs in hPSCs, we aimed to identify miRNAs that are regulated by E2Fs in these cells. We first found that mRNA expression levels of canonical E2F repressors were more expressed than most E2F activators in G1-arrested hPSCs. Moreover, we observed higher mRNA and protein expression levels of canonicalE2F2,E2F3A,andE2F5in G1 synchronized hPSCs compared to human fibroblasts (HF). However,E2F1andE2F4protein expression levels were higher in HF. We next found that E2F inhibition with HLM006474 induced an increase in the G1 cell population without affecting hPSC viability, concomitantly with a decrease inOCT-4mRNA levels and the percentage of OCT-4+hPSCs. Next, by RNA-seq analysis we found 52 differentially expressed (DEGs) miRNAs in HLM006474-treated hESCs. RT-qPCR validation of some of the DEGs let us conclude that miR-19a-3p, miR-19b-3p, miR-4454, miR-1260a, miR-1260b, miR-454-3p and miR-301a-3p are regulated by E2Fs in hPSCs. Interestingly, gene target and ontology analysis of these miRNAs revealed a possible implication in proliferation and cell cycle regulation, development, and neural differentiation.

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Pluripotency-Associated microRNAs in Early Vertebrate Embryos and Stem Cells

Cited by 2 publications

References 155 publications

Unraveling the Multifaceted Roles of Extracellular Vesicles: Insights into Biology, Pharmacology, and Pharmaceutical Applications for Drug Delivery

Unraveling the Multifaceted Roles of Extracellular Vesicles: Insights into Biology, Pharmacology, and Pharmaceutical Applications for Drug Delivery

“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”

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