Plumular meristem transformation system for chickpea: an efficient method to overcome recalcitrant tissue culture responses

Ganguly, Shreeparna; Ghosh, Gourab; Ghosh, Sanatan; Purohit, Arnab; Chaudhuri, Rituparna Kundu; Das, Sampa; Chakraborti, Dipankar

doi:10.1007/s11240-020-01873-8

Cited by 14 publications

(5 citation statements)

References 34 publications

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“…Following this step, the plants are moved to pots and grown under greenhouse conditions until reaching the T 1 generation. The transformation rates using the plumular meristem strategy method were 44% and 72% in the T 1 generation of chickpea [22] and pigeon pea [147], respectively. A similar protocol to the plumular meristem method was developed for alfalfa (Medicago sativa) [149].…”

Section: Plumular Meristem Strategymentioning

confidence: 99%

“…As such, multiple highly efficient in planta research articles performing callus regeneration under in vivo conditions have been published over the years [16][17][18][19][20]. Similarly, several effective in planta protocols using minimal in vitro steps have also been published [21][22][23][24][25]. Conceptually speaking, these published methods all fall within the scope of in planta transformation and were self-described as in planta by their authors; however, their methods do not strictly follow the definitions mentioned above.…”

Section: Definition Of In Planta Stable Transformationmentioning

confidence: 99%

“…Non-chimeric lines are further selected in the T 1 generation. (B) Plumular meristem approach [22,148]. In the plumular meristem approach, young seedlings are decapitated and their radicules excised with a sterile scalpel.…”

Section: Means Of In Planta Transformationmentioning

confidence: 99%

“…Amongst the different protocols using direct de novo shoot organogenesis, the plumular meristem strategy was proposed as a time-efficient direct regenerationbased transformation approach with high transformation rates for chickpea [22,146] and pigeon pea [147,148]. In this system, three-day-old seedlings are decapitated at the shoot apex and pricked in the apical portion and cotyledonary nodes [22,147] (Fig. 6b).…”

Section: Plumular Meristem Strategymentioning

confidence: 99%

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A comprehensive review of in planta stable transformation strategies

Bélanger,

Copley,

Hoyos-Villegas

et al. 2024

Plant Methods

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Plant transformation remains a major bottleneck to the improvement of plant science, both on fundamental and practical levels. The recalcitrant nature of most commercial and minor crops to genetic transformation slows scientific progress for a large range of crops that are essential for food security on a global scale. Over the years, novel stable transformation strategies loosely grouped under the term “in planta” have been proposed and validated in a large number of model (e.g. Arabidopsis and rice), major (e.g. wheat and soybean) and minor (e.g. chickpea and lablab bean) species. The in planta approach is revolutionary as it is considered genotype-independent, technically simple (i.e. devoid of or with minimal tissue culture steps), affordable, and easy to implement in a broad range of experimental settings. In this article, we reviewed and categorized over 300 research articles, patents, theses, and videos demonstrating the applicability of different in planta transformation strategies in 105 different genera across 139 plant species. To support this review process, we propose a classification system for the in planta techniques based on five categories and a new nomenclature for more than 30 different in planta techniques. In complement to this, we clarified some grey areas regarding the in planta conceptual framework and provided insights regarding the past, current, and future scientific impacts of these techniques. To support the diffusion of this concept across the community, this review article will serve as an introductory point for an online compendium about in planta transformation strategies that will be available to all scientists. By expanding our knowledge about in planta transformation, we can find innovative approaches to unlock the full potential of plants, support the growth of scientific knowledge, and stimulate an equitable development of plant research in all countries and institutions.

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Section: Plumular Meristem Strategymentioning

confidence: 99%

Section: Definition Of In Planta Stable Transformationmentioning

confidence: 99%

Section: Means Of In Planta Transformationmentioning

confidence: 99%

Section: Plumular Meristem Strategymentioning

confidence: 99%

See 2 more Smart Citations

A comprehensive review of in planta stable transformation strategies

Bélanger,

Copley,

Hoyos-Villegas

et al. 2024

Plant Methods

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“…Moreover, according to Aasim et al (2013), in vitro regeneration of chickpea is difficult showing minimal success and there is an urgent need to develop a regeneration protocol that can ensure the production of qualitative superior fertile plants. In fact, it is widely regarded, like the other grain legumes, as a highly particularly recalcitrant crop for in vitro tissue culture, which requires modern plant regeneration strategies for genetic transformation and selection of improved cultivars (Hussain et al, 2000;Ganguly et al, 2020).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

In vitro callogenesis essays to induce somatic embryogenesis of the Tunisian chickpea

KAHLAOUI

Bouamama-Gzara

BOKRI

et al. 2022

Not Bot Horti Agrobo

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The present study was the first report on the somatic embryogenesis of the Tunisian chickpea (Cicer arietinum L.) particularly supposed to be recalcitrant and difficult to manipulate in vitro. An efficient protocol has been developed for inducing indirect somatic embryogenesis derived from immature zygotic embryo axis, young leaflet and hypocotylar explants. Callogenesis was achieved on full-strength Murashige and Skoog's (1962) (MS) basal medium supplemented with two auxin/cytokinin combinations; 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-furfurylamonopurine (KIN) or 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) to establish the phytohormones requirement to promote the best induction of friable calli. For somatic embryos induction, embryogenic calli developed from zygotic embryo axes, leaflet and hypocotylar explants were cultured on MS full strength and MS/2, half strength, free of phytohormones media. This study found that all explants exhibited high frequency of callus induction. For immature zygotic embryo axes and young leaflet explants, media containing the combination of 2,4-D and KIN were best effective for inducing callogenesis and friable calli. The most important embryogenic callus percentages were obtained on the culture medium containing the highest concentrations of 2,4-D (2 mg L-1) and KIN (0.5 mg L-1). However, media containing the combination NAA and BAP were best effective for inducing embryogenic calli on hypocotylar explants. A maximal rate of embryogenic calli was reached on medium containing 3 mg L-1 NAA and 1 mg L-1 BAP. The highest number of somatic embryos was obtained with embryogenic calli derived from embryo axis explants and cultured on MS half strength free phytohormones medium. About 56% of somatic embryos converted successfully into fertile plantlets.

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Tackling Lentil Biotic Stresses in the Genomic Era

Vega

Garcı́a

Gutiérrez-González

et al. 2022

Genomic Designing for Biotic Stress Resistant Pulse Crops

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Lentils are already one of the main pulses of the world, as they are one of the main sources of protein for humans. As a crop, they are also gaining momentum because the rusticity and tolerance to water scarcity of some varieties are a good fit with the current global warming trend and climate change in general. However, while the harvested area and overall production have drastically increased over the last decades, yield has only experienced very modest increments. The reasons are two-fold. First, pathogens are affecting the crop as never before, likely due to not only the changing climate but also to the expansion of lentil cultivation to new geographic areas. Second, genomics-aided breeding is far behind many other crops. This is in partly due to the lack of genomic tools currently available to researchers. Progress is being made to adopt high-throughput genomic methods, and researchers will be able to tackle lentil gene discovery and breeding for pathogen resistance and other biotic stresses more efficiently in the coming years. We outline the current situation, novel findings, and prospects of lentil research for biotic stresses. IntroductionLentil (Lens culinaris Medik. subsp. culinaris) is one of the first domesticated species in the Fertile Crescent and along with barley, emmer wheat and einkorn wheat, pea, chickpea, and flax was part of the set of crops that defined the beginnings of the Neolithic transition to agriculture in this part of the World. The origin of the cultivated form is the wild L. culinaris subsp. orientalis (Boiss.) Ponert (syn. L. orientalis Boiss.). A recent publication by Liber et al. (2021) suggests that phylogenetics, population structure, and archeological data coincide in a lentil domestication prolonged in time in Southwest Asia, with two different domesticated gene pools. From the

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Plumular meristem transformation system for chickpea: an efficient method to overcome recalcitrant tissue culture responses

Cited by 14 publications

References 34 publications

A comprehensive review of in planta stable transformation strategies

A comprehensive review of in planta stable transformation strategies

In vitro callogenesis essays to induce somatic embryogenesis of the Tunisian chickpea

Tackling Lentil Biotic Stresses in the Genomic Era

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