PLP1 may serve as a potential diagnostic biomarker of uterine fibroids

Cai, Lei; Liao, Zhiqi; Li, Shiyu; Wu, Ruxing; Li, Jie; Ren, Fang; Zhang, Hanwang

doi:10.3389/fgene.2022.1045395

Cited by 3 publications

(2 citation statements)

References 59 publications

(73 reference statements)

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“…Immunohistochemical staining supports the association between 8-OH-dG and fibroids, as significantly higher levels are seen in human fibroid biopsy tissue compared to normal myometrial tissue [ 63 ]. Moreover, a more recent study examining the gene expression profiles of human fibroid tissues compared to normal myometrial tissue from the Gene Expression Omnibus database determined that proteolipid protein 1 (PLP1), a member of the TNF superfamily, had upregulated mRNA and protein levels in fibroid tissues compared to normal tissues [ 64 ]. Therefore, PLP1 could serve as a useful diagnostic tool for identifying patients with uterine fibroids [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress and Antioxidants in Uterine Fibroids: Pathophysiology and Clinical Implications

et al. 2023

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In the last few decades, our understanding of the complex pathobiology of uterine fibroid development has grown. While previously believed to be a purely neoplastic entity, we now understand that uterine fibroids possess different and equally important aspects of their genesis. An increasing body of evidence suggests that oxidative stress, the imbalance between pro- and antioxidants, is an important factor in fibroid development. Oxidative stress is controlled by multiple, interconnecting cascades, including angiogenesis, hypoxia, and dietary factors. Oxidative stress in turn influences fibroid development through genetic, epigenetic, and profibrotic mechanisms. This unique aspect of fibroid pathobiology has introduced several clinical implications, both diagnostic and therapeutic, that can aid us in managing these debilitating tumors by using biomarkers as well as dietary and pharmaceutical antioxidants for diagnosis and treatment. This review strives to summarize and add to the current evidence revealing the relationship between oxidative stress and uterine fibroids by elucidating the proposed mechanisms and clinical implications.

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Section: Discussionmentioning

confidence: 99%

Oxidative Stress and Antioxidants in Uterine Fibroids: Pathophysiology and Clinical Implications

et al. 2023

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“…The 3,3-diaminobenzidine tetrahydrochloride (DAB) substrate-chromogen system was used to detect the peroxidase activity. The following calculation of all slides was derived from the previous report ( 32 ).…”

Section: Methodsmentioning

confidence: 99%

An autophagy-related diagnostic biomarker for uterine fibroids: FOS

Cai

et al. 2023

Front. Med.

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Uterine fibroids (UFs) are the most common benign gynecologic tumors in reproductive-aged women. The typical diagnostic strategies of UFs are transvaginal ultrasonography and pathological feature, while molecular biomarkers are considered conventional options in the assessment of the origin and development of UFs in recent years. Here, we extracted the differential expression genes (DEGs) and differential DNA methylation genes (DMGs) of UFs from the Gene Expression Omnibus (GEO) database, GSE64763, GSE120854, GSE45188, and GSE45187. 167 DEGs with aberrant DNA methylation were identified, and further Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed by the relevant R package. We next discerned 2 hub genes (FOS, and TNFSF10) with autophagy involvement by overlapping 167 DEGs and 232 autophagic regulators from Human Autophagy Database. FOS was identified as the most crucial gene through the Protein–Protein Interactions (PPI) network with the correlation of the immune scores. Moreover, the down-regulated expression of FOS in UFs tissue at both mRNA and protein levels was validated by RT-qPCR and immunohistochemistry respectively. The area under the ROC curve (AUC) of FOS was 0.856, with a sensitivity of 86.2% and a specificity of 73.9%. Overall, we explored the possible biomarker of UFs undergoing DNA—methylated autophagy and provided clinicians with a comprehensive assessment of UFs.

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