PLP inhibits the activity of interphase centrosomes to ensure their proper segregation in stem cells

Abstract

PLP levels are regulated by Centrobin and act to suppress mother centrosome maturation by blocking the localization of Polo kinase.

The Centrosome and Its Duplication Cycle

“…The centriolar protein, Cep135, is required to establish centrosome asymmetry in Drosophila neuroblasts through shedding of Polo from the mother centrosome (Singh et al 2014). Dplp ( pericentrin-like protein), on the other hand, is more enriched on the mother centrosome (Lerit and Rusan 2013). In Dplp mutant neuroblasts, the mother centrosome retains g-tubulin and Polo levels comparable to the daughter.…”

The Mechanism of Dynein Light Chain LC8-mediated Oligomerization of the Ana2 Centriole Duplication Factor

“…Asymmetry is achieved, in part, via differential maturation of the centrosomes. The daughter centrosome forms the LC8-Ana2-Mud complex that coordinates spindle alignment with cortical polarity cues to maintain a stem population (ganglion mother cell (GMC)) (53,24). E, full-length Drosophila Ana2 has an N-terminal Sas-4 binding region (13,14) and a C-terminal STAN motif (12) conserved across functional Ana2 orthologs.…”

Live Imaging of <em>Drosophila</em> Larval Neuroblasts

“…Our lab has simplified the whole mount preparation of live larval brains for long-term imaging 30,31 . The main advantage to our protocol over others is its simplicity: our observations indicate neither serum, ascorbic acid, insulin, nor fat bodies are necessary additives to image successive rounds of NB ACD.…”