Ploidy level and proliferative activity measurements in a series of 407 thyroid tumors or other pathologic conditions

Salmon, Isabelle; Gasperin, Plínio; Remmelink, Myriam; Rahier, Isabelle; Rocmans, Pierre Arthur; Pasteels, Jean Lambert; Heimann, R; Kiss, Róbert

doi:10.1016/0046-8177(93)90143-5

Cited by 27 publications

(14 citation statements)

References 28 publications

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“…our study on PDTC and ATC, as in others (22)(23)(24), DNA aneuploidy was found in a high proportion of tumours (69.2%), occurring nearly always in anaplastic carcinomas, and most frequently in female patients (only 1 of 5 men presented aneuploid tumours). Jónasson et al (25), in their large flow cytometric study on paraffin material of 494 thyroid carcinomas, showed that aneuploidy was present in 22 of 37 (78.6%) anaplastic tumours.…”

mentioning

confidence: 77%

Aneuploidy and high S-phase as biomarkers of poor clinical outcome in poorly differentiated and anaplastic thyroid carcinoma

Pinto

Silva²,

Banito³

et al. 1994

Oncol Rep

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Abstract. This study aimed to investigate the prognostic influence of DNA flow cytometry and RAS gene mutations in patients with poorly differentiated (PDTC) and anaplastic thyroid carcinoma (ATC). The series consisted of 26 patients with PDTC and ATC, and a median follow-up of 10 months (range 1-138). DNA ploidy and S-phase fraction (SPF) were assessed by flow cytometry on frozen samples. RAS point mutations were detected using PCR techniques. Disease staging and tumour angioinvasion were included as prognostic parameters for survival analysis. Nineteen patients (73.1%) succumbed to the disease (median time 5 months; range 1-45). Eighteen tumours (69.2%) were classified as DNA aneuploid. Median SPF was 5.6% (range 1.9-23.1), which was used as a cut-off value to distinguish between low versus high cell proliferation. Three of 20 (15%) patients presented N-RAS gene mutations in codon 61. DNA aneuploidy was most frequently found in female patients (p=0.034). Kaplan-Meier and Cox regression analyses showed that only DNA aneuploidy (p=0.044 and p=0.055, respectively) and high SPF (p=0.001 and p=0.006, respectively) significantly correlated with worse survival. The results indicate that aneuploidy and high SPF are biomarkers of poor clinical outcome in PDTC and ATC, which may provide useful prognostic information with a potentially therapeutic impact in patient management.

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mentioning

confidence: 77%

Aneuploidy and high S-phase as biomarkers of poor clinical outcome in poorly differentiated and anaplastic thyroid carcinoma

Pinto

Silva²,

Banito³

et al. 1994

Oncol Rep

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“…We have already carried out a number of studies to address the issue on a malignant transformation in certain follicular adenomas of the thyroid gland. We used computer-assisted microscopy analyses of Feulgen-stained nuclei in a series of 238 thyroid lesions with the aim of quantitatively describing morphometric (nuclear area) and textural (chromatin pattern) morphonuclear characteristics (42), and nuclear DNA content (43). These two studies suggest a preneoplastic nature of microfollicular adenomas.…”

Section: Discussionmentioning

confidence: 99%

“…Adenomas exhibit various architectural patterns; four categories are standardly described, including the trabecular/solid subtype (without follicles and in which the cells grow in a diffuse way), the microfollicular subtype (showing neoplastic follicles smaller than the follicles of the normal thyroid gland), the normofollicular subtype (with neoplastic follicles similar in size than the follicles of the normal gland), and the macrofollicular subtype (exhibiting large follicles similar to those in hyperplastic nodules). An admixture of the latter two subtypes (labeled normomacrofollicular) is also commonly seen (22,42,43,45,46). Our series of adenomas included normomacrofollicular (MAC_AD: n ϭ 8), microfollicular (MIC_AD; n ϭ 19), or trabecular (TRA_AD; n ϭ 5) patterns.…”

Section: Histopathological Diagnosesmentioning

confidence: 99%

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Changes in Galectin-7 and Cytokeratin-19 Expression during the Progression of Malignancy in Thyroid Tumors: Diagnostic and Biological Implications

et al. 2002

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Galectin-7 is associated with p53-dependent onset of apoptosis and proliferation control/differentiation in keratinocyte development. It is also upregulated in chemically induced rat mammary carcinogenesis. Because the levels of expression of galectin-7 have never been investigated in thyroid tumors (in contrast to those of galectin-1 and -3 associated with malignancy), we initiated analysis of the expression of galectin-7 in benign and malignant thyroid lesions together with that of cytokeratin-19 (CK19), a marker already demonstrated to be useful in diagnosing this kind of lesion. The immunohistochemical expression levels were quantitatively determined by means of computerassisted microscopy on a series of 84 thyroid lesions including 10 multinodular goiters, 32 adenomas, and 42 carcinomas. Our data clearly indicate a marked down-regulation of galectin-7 expression in a large proportion of adenomas (including the normomacrofollicular, microfollicular, and trabecular variants) if compared with carcinomas. In accordance with results of previous studies, a marked up-regulation of CK19 expression was observed in the thyroid carcinomas, and this contrasted in particular with the low CK19 expression observed in the microfollicular adenomas. Of importance for diagnostic implications, the combination of these two markers enabled our series of microfollicular adenomas (characterized by low galectin-7 and CK19 expression) to be efficiently distinguished from the encapsulated follicular variant of papillary thyroid carcinomas (high galectin-7 and CK19 expression).

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“…The DI thus equals 1.00 in the case of diploid tissue. From the DNA histogram it is also possible to determine the proliferation index (PI), a numerical vari-able representing the percentage of cells in the S phase of the cell cycle (17,24). The PI corresponds to the area under the curve between the G1 and G2 peaks and was calculated as described elsewhere (17,24).…”

Section: Image Cytometrymentioning

confidence: 99%

Discrimination between chronic pancreatitis and pancreatic adenocarcinoma using artificial intelligence-related algorithms based on image cytometry-generated variables

Yeaton

Sears

Ledent³

et al. 1998

Cytometry

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Ploidy level and proliferative activity measurements in a series of 407 thyroid tumors or other pathologic conditions

Cited by 27 publications

References 28 publications

Aneuploidy and high S-phase as biomarkers of poor clinical outcome in poorly differentiated and anaplastic thyroid carcinoma

Aneuploidy and high S-phase as biomarkers of poor clinical outcome in poorly differentiated and anaplastic thyroid carcinoma

Changes in Galectin-7 and Cytokeratin-19 Expression during the Progression of Malignancy in Thyroid Tumors: Diagnostic and Biological Implications

Discrimination between chronic pancreatitis and pancreatic adenocarcinoma using artificial intelligence-related algorithms based on image cytometry-generated variables

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