Ploidy in head and neck cancer: a review and meta-analysis

Stell, P. M.

doi:10.1111/j.1365-2273.1991.tb01051.x

Cited by 31 publications

(9 citation statements)

References 25 publications

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“…This points to an early accumulation of generalized genetic changes in head and neck tumorigenesis, and indicates that the detection of chromosomal aberrations in cytologic specimens is a relevant marker for risk assessment in (pre)clinical stages of this disease. Studies using flow cytometry to measure the DNA content of HNSCC showed that 60‐70% of these tumors were DNA aneuploid 12. In the current study 9 of 12 analyzed solid tumor tissue specimens showed DNA aneuploidy (75%) and chromosomal aberrations were detected in all these tissue specimens.…”

Section: Discussionsupporting

confidence: 93%

Detection of chromosomal aberrations in cytologic brush specimens from head and neck squamous cell carcinoma

Veltman

Hopman

Bot

et al. 1997

Cancer

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METHODS. Brush specimens taken from the tumors of 20 patients with head and neck squamous cell carcinoma (HNSCC) and from the normal mucosa of 8 control 1 Department of Otorhinolaryngology and Head patients were analyzed by FISH using DNA probes for the chromosomes 1 and 7. and Neck Surgery, University Hospital Maas-The FISH results were compared with DNA flow cytometry and FISH results of the tricht and University of Maastricht, Maastricht, The Netherlands. solid tumor specimens. RESULTS. The results of this study showed that 15 of the 20 tumor brush specimens 2 Department of Molecular Cell Biology and Ge-contained numeric chromosomal aberrations in at least 5% of the cells collected. netics, University of Maastricht, Maastricht, The Chromosomal aberrations were detected in all brush specimens taken from tumors Netherlands. that were DNA aneuploid and showed aneusomy. The presence of these aberra-3 Department of Pathology, University Hospital tions correlated well with the classification ''suspicious for malignancy,'' which Maastricht, Maastricht, The Netherlands. was based on Papanicolaou stained slides of the same specimens. In the control group the percentage of chromosomally aberrant cells did not exceed 2%; in addition , no suspiciously malignant cells were observed in this group. CONCLUSIONS. This study reveals that the FISH technique can be applied diagnostically to brush specimens of HNSCC. The presence of chromosomal aberrations in ú 5% of the cells in these specimens can be considered as a marker for malignancy. KEYWORDS: fluorescence in situ hybridization, head and neck squamous cell carci-noma, cytology, DNA flow cytometry. T Presented in part at the 188 th Meeting of the he detection of genetic changes in the cytologic specimens of persons at high risk for the development of head and neck carcinoma in these persons. Epithelial cells can be obtained in a simple, noninva-sive manner by brushing the mucosa. Although exfoliate cytology The authors wish to thank Dr. E. J. M. Speel for using standard Papanicolaou staining is a valuable tool for the diagno-his useful advice on technical matters and for sis of several types of premalignancies and can be used in the head proofreading this article. and neck region for tumor diagnosis, the validity of the method de-Address for reprints: Joris A. Veltman, M.Sc., pends strongly on the representativity of the specimen and the experi-Department of Otorhinolaryngology and Head ence of the investigator. 1,2 Therefore, additional techniques have to and Neck Surgery, University Hospital Maas-be applied to obtain more objective diagnostic criteria. Fluorescence tricht, P.O. Box 5800, 6202 AZ Maastricht, The in situ hybridization (FISH) can be used to study the chromosomal Netherlands. constitution of cytologic specimens 3 and recently has been reported to be of use in the cytopathologic diagnosis of cervical neoplasia. 4,5

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Section: Discussionsupporting

confidence: 93%

Detection of chromosomal aberrations in cytologic brush specimens from head and neck squamous cell carcinoma

Veltman

Hopman

Bot

et al. 1997

Cancer

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“…'~ In the current study, a modified system was used, originally designed for oral squamous cell carcinoma, and including three variables for each tumor cell population and each tumor-host relationship. *' Each variable was rated on a 4-point scale (1)(2)(3)(4), the aggregate score thus ranging from 6-24 points depending on the tumor's malignancy grade ( Table 2). The variables were adapted in such a way that all types of squamous cell carcinoma could be fitted in to the scheme.…”

Section: Histopathologic Malignancy Gradementioning

confidence: 99%

Chromosomal abnormalities involving 11q13 are associated with poor prognosis in patients with squamous cell carcinoma of the head and neck

Åkervall

Jin

Wennerberg

et al. 1995

Cancer

104

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Background. In individual patients with squamous cell carcinoma of the head and neck (SCCHN), established prognostic factors do not satisfactorily predict clinical outcome. Although the karyotype is an independent prognostic factor in certain hematologic malignancies and solid tumors, no data have been reported concerning the possible relationship between chromosomal abnormalities and clinical outcome in patients with SCCHN. Methods. In 116 cases of primary SCCHN, short term cultures were analyzed cytogenetically during 1987 through 1991, the karyotypes were divided into four groups: k1, normal (n = 35); k2, numeric changes only (n = 31); k3, simple structural abnormalities (n = 27); and k4, complex karyotypes (n = 23). The patients were followed for at least 18 months after diagnosis or until death. Results. The 2‐year survival rate was lower in the k4 subgroup (35%) than in the k1, k2, and k3 subgroups taken together (61%), both in the series as a whole (P = 0.02), and in the largest tumor site subgroup, laryngeal squamous cell carcinoma (n = 32), (P = 0.04). The most prevalent breakpoint was in chromosome band 11q13, occurring in 11 tumors, 10 of which belonged to the k4‐subgroup. The 2‐year survival rate was lower for patients with 11q13 rearrangements (20%) than for those without (60%), both in the series as a whole (P = 0.001), and in the k4‐subgroup (P = 0.02). Conclusions. The results suggest that in SCCHN the presence of a complex karyotype is associated with poor prognosis, particularly when 11q13 rearrangements are pres.

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“…However, Kokal, et al 4 noted that patients with aneuploid squamous cell carcinoma had significantly decreased relapse‐free and overall survival rates compared to patients with diploid tumors. In other studies, 7 aneuploidy was found to be of no importance in predicting clinical outcome.…”

Section: Introductionmentioning

confidence: 77%

Retrospective DNA Ploidy Analysis by Image and Flow Cytometry in Head and Neck Cancer

et al. 1996

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A retrospective analysis of DNA content (DNA ploidy) was conducted in formalin-fixed specimens from patients with advanced, unresectable head and neck cancer who were treated on a single defined protocol with accelerated fractionation radiation therapy and concomitant cisplatin (Platinol) chemotherapy. Specimens from 31 tumor sites were analyzed by image cytometry using the Feulgen staining method. Fifteen specimens were analyzed by flow cytometry after deparaffinization, nuclear disaggregation, and staining with propidium iodide. By image analysis, 10 (32%) of 31 specimens contained only diploid tumor cells, while 21 (68%) specimens exhibited at least one aneuploid tumor component. Seven of the eight tumors with a single G0/G1 peak by image analysis had a single peak by flow analysis. Five of the seven tumors with multiple peaks by image analysis had multiple peaks by flow analysis. Histology was also reevaluated, and tumor grade was determined, reflecting tumor cell differentiation based on keratinization, mitotic activity and the degree of nuclear polymorphism. For this well-defined patient population managed according to a uniform therapeutic approach, DNA ploidy status and histology provided a suggestion of prognostic separation; however, statistical significance was not obtained, most likely due to the small number of patients.

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Ploidy in head and neck cancer: a review and meta-analysis

Cited by 31 publications

References 25 publications

Detection of chromosomal aberrations in cytologic brush specimens from head and neck squamous cell carcinoma

Detection of chromosomal aberrations in cytologic brush specimens from head and neck squamous cell carcinoma

Chromosomal abnormalities involving 11q13 are associated with poor prognosis in patients with squamous cell carcinoma of the head and neck

Retrospective DNA Ploidy Analysis by Image and Flow Cytometry in Head and Neck Cancer

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