Ploidy estimation in Hemerocallis species and cultivars by flow cytometry

Saito, Hiroyuki; Mizunashi, Keiko; Tanaka, Shigefumi; Adachi, Yukiko; Nakano, Masataka

doi:10.1016/s0304-4238(02)00150-4

Cited by 24 publications

(18 citation statements)

References 16 publications

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“…The relative DNA contents of nuclei isolated from leaf tissues were measured using a flow cytometer PA (Partec GmbH, Münster, Germany) according to Saito et al (2003). Briefly, young leaf segments (ca.…”

Section: Fcm Analysismentioning

confidence: 99%

“…For FCM analysis, samples should not be large, and thus this method enables the early detection of hybrids. FCM analysis also has the advantages of accuracy, convenience, simplicity, and quickness as compared with conventional chromosome observation 74 (Dolezel, 1997;Ozaki et al, 2004;Saito et al, 2003;Roux et al, 2003;Shiba and Mii, 2005), and thus a large number of samples can be analyzed in a short period of time. Although FCM analysis was also performed for hybrid identification in intergeneric hybridization between S. aurantiaca Hook.…”

Section: Introductionmentioning

confidence: 99%

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Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses

淳二

幸子

陽子

et al. 2007

Engei Gakkai Zasshi

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Plantlets derived from ovule culture following intra-or intergeneric crosses among Colchicaceous ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., were subjected to flow cytometry (FCM) and random amplified polymorphic DNA (RAPD) analyses in order to verify their hybridity. For crosses between plants with apparently distinct relative fluorescence intensity (RFI) of nuclei, i.e., intrageneric crosses between Gloriosa genotypes with different ploidy levels, and most intergeneric crosses, detection of hybrid plantlets could be readily accomplished by FCM analysis. The results of hybrid identification by RAPD analysis supported those of FCM analysis. In addition, RAPD analysis allowed the verification of the hybridity of intra-or intergeneric cross-derived plantlets, which could not be identified as hybrids by FCM analysis due to the similarity of RFI of the parents or appearance of the RFI peak in an unexpected position. Totally, 110 independent hybrid plantlets (60 intrageneric and 50 intergeneric hybrids) have so far been identified by FCM and/or RAPD analyses. Thus, FCM in combination with RAPD analyses offer simple and rapid means for the early detection of intrageneric and intergeneric hybrids in Colchicaceous ornamentals.

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Section: Fcm Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses

淳二

幸子

陽子

et al. 2007

Engei Gakkai Zasshi

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“…All of the daylily tetraploid plants have been obtained by induction with colchicine (Arisumi 1972;Chen and Goeden-Kallemeyn 1979). No naturally occurring tetraploid daylilies have ever been reported (Saito et al 2003;Gulia et al 2009;Zhang et al 2013). Instead, several taxa with the Hemerocallis species complex are naturally occurring triploids (2n = 3x = 33) (Gulia et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and genome size changes (variation) in synthetic tetraploids of daylily (Hemerocallis) in relation to their diploid counterparts

et al. 2014

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Tetraploids of daylily have taken a leading position among the daylily cultivars due to desirable traits such as vigorous growth and flowers with more intense colour. In our previous studies, several tetraploids of daylily cultivars 'Blink of an Eye' and 'Berlin Multi' were obtained using in vitro techniques with different antimitotic agents (colchicine, oryzalin, trifluralin, and amiprophos methyl). The purposes of this study were to evaluate changes in daylily tetraploids in relation to their diploid counterparts and to assess variation among diploids and tetraploids derived from genetically homogenous plant material treated with antimitotic agents. In the first year of the ex vitro cultivation, growth of tetraploids was poorer in comparison with diploids, but in the second year, tetraploid growth was much more vigorous. Compared to diploids, in tetraploids of both cultivars percentage of flowering plants was lower, flowering was delayed by 8 days and 1 month in 'Blink of an Eye' and 'Berlin Multi', respectively, and bud number per scape was lower by approximately 20 and 40 %, respectively. Tetraploid leaves and flowers of both cultivars were significantly larger, chlorophyll concentration index was higher by approximately 40 %, and stomata were longer by 35 %. In 'Berlin Multi' tetraploids, inflorescence stems were shorter by 20 %. In 'Blink of an Eye', variation in flower colour tone and shape and stamen malformation rate was detected both within the unconverted diploids and tetraploids but was more evident in tetraploids. In both cultivars, variation was also found in the nuclear DNA content, which ranged in diploids and tetraploids, respectively, 8.02-8.53 and 16.01-17.13 pg in 'Berlin Multi ' and 8.28-8.71 and 15.93-17.36 pg in 'Blink of an Eye'. Since the variation, while less evident, also occurred in the diploids (regenerated from the antimitotic treated material), we suppose that these variations could be due to antimitotic agents that can induce not only chromosome doubling but also chromosomal and gene mutations. The extent and character of these changes can be related to parental genotype and/or antimitotic agent. Further research is required at the cytological and molecular level to explain the character of changes, epigenetic and/or genetic.

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“…(Gatt et al, 1998). Flow cytometry (FCM) has been used to determine ploidy levels in a number of plant species, such as Hemerocallis (Saito et al, 2003), Asparagus (González Castañón and Schroeder, 2002), Colchicaceous ornamentals (Amano 336 et al, 2007) and Japanese persimmon (Yamada and Tao, 2007). In the present study, we investigated the ploidy levels of 45 pot carnation cultivars using FCM.…”

Section: Introductionmentioning

confidence: 99%

Estimation of Ploidy Levels and Breeding Backgrounds in Pot Carnation Cultivars Using Flow Cytometry and SSR Markers

Yagi

Kimura

Yamamoto

et al. 2009

J. Japan. Soc. Hort. Sci.

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We estimated the ploidy level of 45 pot carnation cultivars by means of flow cytometry (FCM). The results strongly suggested that 27 cultivars are diploid, 3 are triploid, and 15 are tetraploid. To verify the actual ploidy levels, we counted chromosome numbers in the root tips of one cultivar at each ploidy level. This revealed that 'Camille' was diploid, 'Baby Heart' was triploid, and 'Tula' was tetraploid. Our results suggested that the ploidy level varies among pot carnation cultivars. We analyzed the genotypes of 32 cultivars by using five simple sequence repeat (SSR) markers to estimate the correspondence with FCM ploidy levels and to analyze genetic diversity. Ploidy estimated from the maximum number of alleles per locus corresponded with that estimated by FCM among diploid and triploid cultivars, but not among tetraploids. Among diploid pot cultivars, we found only three or four alleles per locus, and most alleles were common to diploid cut cultivars. On the other hand, triploid and tetraploid pot cultivars had four to eight alleles per locus, and most were unique to given ploidy levels. SSR analysis suggested that diploid pot cultivars were derived from cut cultivars, while triploids and tetraploids were produced by crossing wild Dianthus spp. and similar genetic resources. In total, 30 pot cultivars with different ploidy levels could be successfully identified from the genotype of five SSR markers.

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Ploidy estimation in Hemerocallis species and cultivars by flow cytometry

Cited by 24 publications

References 16 publications

Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, <i>Gloriosa</i> spp., <i>Littonia modesta</i> Hook. and <i>Sandersonia aurantiaca</i> Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses

Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, <i>Gloriosa</i> spp., <i>Littonia modesta</i> Hook. and <i>Sandersonia aurantiaca</i> Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses

Phenotypic and genome size changes (variation) in synthetic tetraploids of daylily (Hemerocallis) in relation to their diploid counterparts

Estimation of Ploidy Levels and Breeding Backgrounds in Pot Carnation Cultivars Using Flow Cytometry and SSR Markers

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