PLLA/POSS Nanofibers Loaded with Multitargeted pANG Composite Nanoparticles for Promotion of Vascularization in Shear Flow

Abstract

In vitro prevascularization is particularly important for the clinical application of tissue engineering scaffolds that require vascularization. The principal challenge is simulating the dynamic in vivo environment to promote the continuous growth of blood vessels. In this study, two targeting polypeptides are linked to the two ends of an amphiphilic block copolymer, polyethyleneimine‐b‐poly(lactide‐co‐3(S)‐methyl‐morpholine‐2,5‐dione)‐b‐polyethyleneimine (PEI‐PLMD‐PEI), and self‐assembled to form positively charged nanoparticles (NPs), which can bind to negatively charged pANG through electrostatic interactions; the polypeptides are finally loaded into PLLA/polyhedral oligomeric silsesquioxane (POSS) porous fibers to prepare untargeted nanofibers (unTFs), targeted porous nanofibers (TFBs), and targeted nanofiber bundles. The effects of the porous nanofibers on human umbilical vein endothelial cell (HUVEC) transfection, spreading, proliferation, morphology, and expression of related factors are investigated under the action of shear flow force. The results show that the PLLA/POSS nanofibers can maintain stable release of multitargeted NPs for nearly 45 days. Both the dual‐targeted porous NPs and shear flow improve the pANG transfection efficiency and promote cell proliferation, and they have a good synergistic effect. These results provide a potential strategy for designing HUVEC‐specific gene carriers and using shear flow to enhance endothelialization.

Recent advances in inhibiting atherosclerosis and restenosis: from pathogenic factors, therapeutic molecules to nano-delivery strategies

“…Similarly, Li et al constructed targeted Ang gene-eluting nanofibers (TFs) via coelectrospinning REDV-PEI-polyethyleneimine(PLMD)/pAng nanoparticles (NPs) to the poly (L-lactic acid) (PLLA)/polyhedral oligomeric silsesquioxane (PLLA/POSS) matrix. 201 Then, targeted gene-eluting nanofiber-bundle (TFB) was obtained by stretching and twisting TFs. Due to the EC-targeting function of REDV, NPs released from TFB could specifically transfect VECs to accelerate endothelialization, which might be attributed to the synergy of the overexpression of Ang gene and the structure of TFB.…”

PLLA–gelatin composite fiber membranes incorporated with functionalized CeNPs as a sustainable wound dressing substitute promoting skin regeneration and scar remodeling

“…Poly-L-Lactic acid (PLLA) and poly(lactic-co-glycolic acid) (PLGA) as FDA approved synthetic polymers have good biocompatibility and biodegradability. 11 It is believed that their degradation products are lactic acid and glycolic acid. 12 Both the products are considered as part of various metabolic pathways under normal physiological conditions in the human body.…”

Electrospun Core–Shell Structure Fibers for Puerarin-Loaded Vascular Grafts

“…Gelatin (Gel) is a protein obtained by the partial hydrolysis of collagen with excellent biocompatibility. , The structure of gelatin is similar to that of the extracellular matrix and supports cell adhesion and differentiation . Its degradation products can be absorbed by the body without causing adverse reactions such as inflammation. − The US Food and Drug Administration has approved poly- l -polylactic acid (PLLA), a semicrystalline polymer, for clinical use due to its long degradation cycle, excellent mechanical properties, and biocompatibility despite its cell compatibility and hydrophobicity being poor. , Multiwalled carbon nanotubes (MWNTs) are columnar molecules composed of hexagonal sp 2 hybridization carbon atoms. It can readily reinforce with other materials due to the large aspect ratio and excellent mechanical properties. − Puerarin (PUE) is present in the roots of the legume Pueraria lobata.…”