Plk4 and Aurora A cooperate in the initiation of acentriolar spindle assembly in mammalian oocytes

Bury, Leah; Coelho, Paula A.; Simeone, Angela; Ferries, Samantha; Eyers, Claire E.; Eyers, Patrick A.; Zernicka‐Goetz, Magdalena; Glover, David M.

doi:10.1083/jcb.201606077

Cited by 59 publications

(83 citation statements)

References 64 publications

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“…The structure of NuMA in acentrosomal cells was similarly formed in various cell lines derived from different human tissues, including nontransformed and hTERT‐immortalized human retinal pigment epithelium cells (RPE1; Appendix Fig S2C and D). It has been reported that PLK4 contributes to microtubule nucleation in oocytes (Bury et al , ). Thus, we examined whether the observed structure of NuMA could be attributed to the inhibition of PLK4 or to the low microtubule density induced by the removal of centrosomes.…”

Section: Resultsmentioning

confidence: 99%

Nu MA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells

et al. 2019

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In most animal cells, mitotic spindle formation is mediated by coordination of centrosomal and acentrosomal pathways. At the onset of mitosis, centrosomes promote spindle bipolarization. However, the mechanism through which the acentrosomal pathways facilitate the establishment of spindle bipolarity in early mitosis is not completely understood. In this study, we show the critical roles of nuclear mitotic apparatus protein (NuMA) in the generation of spindle bipolarity in acentrosomal human cells. In acentrosomal human cells, we found that small microtubule asters containing NuMA formed at the time of nuclear envelope breakdown. In addition, these asters were assembled by dynein and the clustering activity of NuMA. Subsequently, NuMA organized the radial array of microtubules, which incorporates Eg5, and thus facilitated spindle bipolarization. Importantly, in cells with centrosomes, we also found that NuMA promoted the initial step of spindle bipolarization in early mitosis. Overall, these data suggest that canonical centrosomal and NuMA-mediated acentrosomal pathways redundantly promote spindle bipolarity in human cells.

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Section: Resultsmentioning

confidence: 99%

Nu MA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells

et al. 2019

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“…These results suggest that kinases responsible for Pum1 phosphorylation are present and at least partially active in immature oocytes. Polo-like kinase (Plk) 1 and 4 were shown to be present in immature mouse oocytes (Bury et al, 2017; Pahlavan et al, 2000). Interestingly, inhibition of Plk4, but not that of Plk1, prevented the dissolution of Pum1 aggregates (Figs.…”

Section: Resultsmentioning

confidence: 99%

Changes in subcellular structures and states of Pumilio1 regulate the translation of targetMad2andCyclin B1mRNAs

Takei

Takada

Kawamura

et al. 2020

Preprint

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1Temporal and spatial control of mRNA translation has emerged as a major mechanism 2 for promoting diverse biological processes. However, the molecular nature of temporal 3 control of translation remains unclear. In oocytes, many mRNAs are deposited as a 4 translationally repressed form and are translated at appropriate timings to promote the 5 progression of meiosis and development. Here, we show that changes in structures and 6 states of the RNA-binding protein Pumilio1 regulate the translation of target mRNAs 7 and progression of oocyte maturation. Pumilio1 was shown to bind to Mad2 and Cyclin 8 B1 mRNAs, assemble highly clustered solid-like aggregates, and surround Mad2 and 9Cyclin B1 RNA granules in mouse oocytes. These Pumilio1 aggregates were dissolved 10 by phosphorylation prior to the translational activation of target mRNAs. Stabilization 11 of Pumilio1 aggregates prevented the translational activation of target mRNAs and 12 oocyte maturation. Together, our results provide an aggregation-dissolution model for 13 the temporal and spatial control of translation. 14 15 16

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“…Centrosome‐driven and centriole‐independent pathways still require a common set of centrosomal and microtubule assembly promoting factors. The centrosome scaffold proteins Cep152 and Cep192 together with Plk4 are required to organize microtubules despite the absence of centrioles not only in mouse embryos but also during murine meiosis . γ‐TuRC, the key microtubule templating activity, remains essential for spindle formation in the absence or presence of centrosomes (Reviewed in: Ref.…”

Section: Spindle Formation In the Absence Of Centriolesmentioning

confidence: 99%

Loss and Rebirth of the Animal Microtubule Organizing Center: How Maternal Expression of Centrosomal Proteins Cooperates with the Sperm Centriole in Zygotic Centrosome Reformation

2018

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Centrosomes are the main microtubule organizing centers in animal cells. In particular during embryogenesis, they ensure faithful spindle formation and proper cell divisions. As metazoan centrosomes are eliminated during oogenesis, they have to be reassembled upon fertilization. Most metazoans use the sperm centrioles as templates for new centrosome biogenesis while the egg's cytoplasm re-prepares all components for on-going centrosome duplication in rapidly dividing embryonic cells. We discuss our knowledge and the experimental challenges to analyze zygotic centrosome reformation, which requires genetic experiments to enable scrutinizing respective male and female contributions. Male and female knockout animals and mRNA injection to mimic maternal expression of centrosomal proteins could point a way to the systematic molecular dissection of the process. The most recent data suggest that timely expression of centrosome components in oocytes is the key to zygotic centrosome reformation that uses male sperm as coordinators for de novo centrosome production.

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Plk4 and Aurora A cooperate in the initiation of acentriolar spindle assembly in mammalian oocytes

Cited by 59 publications

References 64 publications

Nu MA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells

Nu MA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells

Changes in subcellular structures and states of Pumilio1 regulate the translation of targetMad2andCyclin B1mRNAs

Loss and Rebirth of the Animal Microtubule Organizing Center: How Maternal Expression of Centrosomal Proteins Cooperates with the Sperm Centriole in Zygotic Centrosome Reformation

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