Plipastatin and surfactin coproduction by Bacillus subtilis pB2-L and their effects on microorganisms

Gao, Ling; Han, Jun; Liu, Hongxia; Qu, Xiaoxu; Lu, Zhaoxin; Bie, Xiaomei

doi:10.1007/s10482-017-0874-y

Cited by 48 publications

(35 citation statements)

References 39 publications

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“…B. subtilis has two morphological phenotypes during active growth: filamentous, sessile cells and smaller motile cells (44,45). In liquid media, the bacteria primarily swim which requires the expression of the flagellin protein (hag) and the fla-che operon (Figures 5B).…”

Section: Heterogeneous Activation Of Myo-inositol Catabolism Pathway mentioning

confidence: 99%

Microbial single-cell RNA sequencing by split-pool barcoding

Kuchina

Brettner

Paleologu

et al. 2019

Preprint

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Single-cell RNA-sequencing (scRNA-seq) has become an essential tool for characterizing multicelled eukaryotic systems but current methods are not compatible with bacteria. Here, we introduce microSPLiT, a low cost and high-throughput scRNA-seq method that works for gramnegative and gram-positive bacteria and can resolve transcriptional states that remain hidden at a population level. We applied microSPLiT to >25,000 Bacillus subtilis cells sampled from different growth stages, creating a detailed atlas of changes in metabolism and lifestyle. We not only retrieve detailed gene expression profiles associated with known but rare states such as competence and PBSX prophage induction, but also identify novel and unexpected gene expression states including heterogeneous activation of a niche metabolic pathway in a subpopulation of cells. microSPLiT empowers high-throughput analysis of gene expression in complex bacterial communities.

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Section: Heterogeneous Activation Of Myo-inositol Catabolism Pathway mentioning

confidence: 99%

Microbial single-cell RNA sequencing by split-pool barcoding

Kuchina

Brettner

Paleologu

et al. 2019

Preprint

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“…The NCIB3610 strain does not produce iturins but harbors the operons for surfactin and plipastatin (a member of the fengycin family) production, i.e., the srfA and ppsB operons, respectively (46). However, it has been reported that the promoter of the plipastatin operon in the NCIB3610 isolate and its isogenic derivatives (i.e., strain 168) is weak; thus, surfactin would be the only lipopeptide produced in significant amounts by this wild isolate under laboratory conditions (47,48). Therefore, we investigated whether the ability of B. subtilis NCIB3610 to control the growth of F. verticillioides depended on the proficiency of this strain in producing surfactin.…”

Section: F Verticillioides Activates the Rsbp-dependent Pathway Of Tmentioning

confidence: 99%

Stress-Responsive Alternative Sigma Factor SigB Plays a Positive Role in the Antifungal Proficiency of Bacillus subtilis

Bartolini

Cogliati

Vileta

et al. 2019

Appl Environ Microbiol

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DifferentBacillusspecies with PGPR (plant growth-promoting rhizobacterium) activity produce potent biofungicides and stimulate plant defense responses against phytopathogenic fungi. However, very little is known about how these PGPRs recognize phytopathogens and exhibit the antifungal response. Here, we report the antagonistic interaction betweenBacillus subtilisand the phytopathogenic fungusFusarium verticillioides. We demonstrate that this bacterial-fungal interaction triggers the induction of the SigB transcription factor, the master regulator ofB. subtilisstress adaptation. Dual-growth experiments performed with live or dead mycelia or culture supernatants ofF. verticillioidesshowed that SigB was activated and required for the biocontrol of fungal growth. Mutations in the different regulatory pathways of SigB activation in the isogenic background revealed that only the energy-related RsbP-dependent arm of SigB activation was responsible for specific fungal detection and triggering the antagonistic response. The activation of SigB increased the expression of the operon responsible for the production of the antimicrobial cyclic lipopeptide surfactin (thesrfAoperon). SigB-deficientB. subtiliscultures produced decreased amounts of surfactin, andB. subtiliscultures defective in surfactin production (ΔsrfA) were unable to control the growth ofF. verticillioides.In vivoexperiments of seed germination efficiency and early plant growth inhibition in the presence ofF. verticillioidesconfirmed the physiological importance of SigB activity for plant bioprotection.IMPORTANCEBiological control using beneficial bacteria (PGPRs) represents an attractive and environment-friendly alternative to pesticides for controlling plant diseases. Different PGPRBacillusspecies produce potent biofungicides and stimulate plant defense responses against phytopathogenic fungi. However, very little is known about how PGPRs recognize phytopathogens and process the antifungal response. Here, we report howB. subtilistriggers the induction of the stress-responsive sigma B transcription factor and the synthesis of the lipopeptide surfactin to fight the phytopathogen. Our findings show the participation of the stress-responsive regulon of PGPRBacillusin the detection and biocontrol of a phytopathogenic fungus of agronomic impact.

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“…Surfactin, encoded by the srfAA-AD gene cluster, is a well-studied biosurfactant and is involved in reducing surface tension needed for swarming and sliding motility (16,17). Its cytolytic activity is mainly based on its surfactant activity, causing cell lysis due to penetration of bacterial lipid bilayer membranes and forming ion-conducting channels (9,(18)(19)(20)(21)(22)(23). The powerful antifungal lipopeptide plipastatin, chemically very similar to fengycin, but distinct D-tyrosine position within the peptide backbone, is synthesized by the ppsA-E gene cluster.…”

Section: Introductionmentioning

confidence: 99%

Genomic and chemical diversity ofBacillus subtilissecondary metabolites against plant pathogenic fungi

Kiesewalter

Lozano-Andrade

Wibowo

et al. 2020

Preprint

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Bacillus subtilis produces a wide range of secondary metabolites providing diverse plant-growth-promoting and biocontrol abilities. These secondary metabolites include non-ribosomal peptides (NRPs) with strong antimicrobial properties, causing either cell lysis, pore formation in fungal membranes, inhibition of certain enzymes, or bacterial protein synthesis. However, the natural products of B. subtilis are mostly studied either in laboratory strains or in individual isolates and therefore, a comparative overview of B. subtilis secondary metabolites is missing.In this study, we have isolated 23 B. subtilis strains from eleven sampling sites, compared the fungal inhibition profiles of wild types and their NRPs mutants, followed the production of targeted lipopeptides, and determined the complete genomes of 13 soil isolates. We discovered that non-ribosomal peptide production varied among B. subtilis strains co-isolated from the same soil samples. In vitro antagonism assays revealed that biocontrol properties depend on the targeted plant pathogenic fungus and the tested B. subtilis isolate. While plipastatin alone is sufficient to inhibit Fusarium sp., a combination of plipastatin and surfactin is required to hinder the growth of Botrytis cinerea. Detailed genomic analysis revealed that altered NRP production profiles in certain isolates is due to missing core genes, nonsense mutation, or potentially altered gene regulation.Our study combines microbiological antagonism assays with chemical NRPs detection and biosynthetic gene cluster predictions in diverse B. subtilis soil isolates to provide a broader overview of the secondary metabolite chemodiversity of B. subtilis.IMPORTANCESecondary or specialized metabolites with antimicrobial activities define the biocontrol properties of microorganisms. Members of the Bacillus genus produce a plethora of secondary metabolites, of which non-ribosomally produced lipopeptides in particular display strong antifungal activity. To facilitate prediction of the biocontrol potential of new Bacillus subtilis isolates, we have explored the in vitro antifungal inhibitory profiles of recent B. subtilis isolates, combined with analytical natural product chemistry, mutational analysis, and detailed genome analysis of biosynthetic gene clusters. Such a comparative analysis helped to explain why selected B. subtilis isolates lack production of certain secondary metabolites.

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Plipastatin and surfactin coproduction by Bacillus subtilis pB2-L and their effects on microorganisms

Cited by 48 publications

References 39 publications

Microbial single-cell RNA sequencing by split-pool barcoding

Microbial single-cell RNA sequencing by split-pool barcoding

Stress-Responsive Alternative Sigma Factor SigB Plays a Positive Role in the Antifungal Proficiency of Bacillus subtilis

Genomic and chemical diversity ofBacillus subtilissecondary metabolites against plant pathogenic fungi

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