PLGA nanoparticle preparations by emulsification and nanoprecipitation techniques: effects of formulation parameters

Hernández-Giottonini, Karol Yesenia; Rodríguez-Córdova, Rosalva Josefina; Gutiérrez-Valenzuela, Cindy Alejandra; Peñuñuri-Miranda, Omar; Zavala-Rivera, Paul; Guerrero-Germán, Patricia; Lucero‐Acuña, Armando

doi:10.1039/c9ra10857b

Cited by 162 publications

(140 citation statements)

References 110 publications

(109 reference statements)

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“…In general, two main strategies are employed, namely, the dispersion of preformed polymers or the polymerization of monomers [ 27 , 28 ]. Table 2 lists the most commonly used techniques [ 29 , 30 ].…”

Section: Methods For Production Of Polymeric Nanoparticlesmentioning

confidence: 99%

Polymeric Nanoparticles: Production, Characterization, Toxicology and Ecotoxicology

et al. 2020

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Polymeric nanoparticles (NPs) are particles within the size range from 1 to 1000 nm and can be loaded with active compounds entrapped within or surface-adsorbed onto the polymeric core. The term “nanoparticle” stands for both nanocapsules and nanospheres, which are distinguished by the morphological structure. Polymeric NPs have shown great potential for targeted delivery of drugs for the treatment of several diseases. In this review, we discuss the most commonly used methods for the production and characterization of polymeric NPs, the association efficiency of the active compound to the polymeric core, and the in vitro release mechanisms. As the safety of nanoparticles is a high priority, we also discuss the toxicology and ecotoxicology of nanoparticles to humans and to the environment.

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Section: Methods For Production Of Polymeric Nanoparticlesmentioning

confidence: 99%

Polymeric Nanoparticles: Production, Characterization, Toxicology and Ecotoxicology

et al. 2020

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“…Therefore, according with available literature data, the use of a cryoprotectant agents was required. Usually PLGA nanoparticles can be stabilized using a variety of cryoprotectant, including PVA, PVP and sucrose, at a concentration varying from 2 to 5% w / v [ 30 , 31 , 32 ]. In order to understand which type and concentration of cryoprotectant agent give the best redispersion of PLGA-CDs1% nanoparticles DLS measurements were performed after redispersion of freeze-dried samples containing different common cryoprotectors at different concentrations ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

Carbon Nanodots as Functional Excipient to Develop Highly Stable and Smart PLGA Nanoparticles Useful in Cancer Theranostics

et al. 2020

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Theranostic systems have attracted considerable attention for their multifunctional approach to cancer. Among these, carbon nanodots (CDs) emerged as luminescent nanomaterials due to their exceptional chemical properties, synthetic ease, biocompatibility, and for their photothermal and fluorescent properties useful in cancer photothermal therapy. However, premature renal excretion due to the small size of these particles limits their biomedical application. To overcome these limitations, here, hybrid poly(lactic-co-glycolic acid) (PLGA-CDs) nanoparticles with suitable size distribution and stability have been developed. CDs were decisive in the preparation of polymeric nanoparticles, not only conferring them photothermal and fluorescent properties, needed in theranostics, but also having a strategic role in the stabilization of the system in aqueous media. In fact, CDs provide stable PLGA-based nanoparticles in aqueous media and sufficient cryoprotection in combination with 1% PVP. While PLGA nanoparticles required at least 5% of sucrose. Comparing nanosystems with different CDs content, it is also evident how these positively impinge on the loading and release of the drug, favoring high drug loading (~4.5%) and a sustained drug release over 48 h. The therapeutic and imaging potentials were finally confirmed through in vitro studies on a breast cancer cell line (MDA-MB-231) using fluorescence imaging and the MTS cell viability assay.

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“…Many studies have tried to analyze the difference in nanoparticles generated by solvent evaporation and solvent displacement. Hernández-Giottonini and colleagues [76] evaluated the effect of process parameters and formulation parameters on polylactic-co-glycolic acid (PLGA) nanoparticles prepared by both techniques. While particle size was dominantly affected by PLGA and PVA concentrations for the nanoprecipitation method, solvent fraction had the most effect of the particle size for the solvent evaporation technique.…”

Section: Nanoprecipitationmentioning

confidence: 99%

Natural Polymers in Micro- and Nanoencapsulation for Therapeutic and Diagnostic Applications: Part I: Lipids and Fabrication Techniques

Ngwuluka¹,

Abu-Thabit²,

Uwaezuoke³

et al. 2021

Nano- And Microencapsulation - Techniques and Applications

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Encapsulation, specifically microencapsulation is an old technology with increasing applications in pharmaceutical, agrochemical, environmental, food, and cosmetic spaces. In the past two decades, the advancements in the field of nanotechnology opened the door for applying the encapsulation technology at the nanoscale level. Nanoencapsulation is highly utilized in designing effective drug delivery systems (DDSs) due to the fact that delivery of the encapsulated therapeutic/diagnostic agents to various sites in the human body depends on the size of the nanoparticles. Compared to microencapsulation, nanoencapsulation has superior performance which can improve bioavailability, increase drug solubility, delay or control drug release and enhance active/passive targeting of bioactive agents to the sites of action. Encapsulation, either micro- or nanoencapsulation is employed for the conventional pharmaceuticals, biopharmaceuticals, biologics, or bioactive drugs from natural sources as well as for diagnostics such as biomarkers. The outcome of any encapsulation process depends on the technique employed and the encapsulating material. This chapter discusses in details (1) various physical, mechanical, thermal, chemical, and physicochemical encapsulation techniques, (2) types and classifications of natural polymers (polysaccharides, proteins, and lipids) as safer, biocompatible and biodegradable encapsulating materials, and (3) the recent advances in using lipids for therapeutic and diagnostic applications. Polysaccharides and proteins are covered in the second part of this chapter.

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PLGA nanoparticle preparations by emulsification and nanoprecipitation techniques: effects of formulation parameters

Abstract: This study presents the influence of the primary formulation parameters on the formation of poly-dl-lactic-co-glycolic nanoparticles by the emulsification-solvent evaporation, and the nanoprecipitation techniques.

Cited by 162 publications

References 110 publications

Polymeric Nanoparticles: Production, Characterization, Toxicology and Ecotoxicology

Polymeric Nanoparticles: Production, Characterization, Toxicology and Ecotoxicology

Carbon Nanodots as Functional Excipient to Develop Highly Stable and Smart PLGA Nanoparticles Useful in Cancer Theranostics

Natural Polymers in Micro- and Nanoencapsulation for Therapeutic and Diagnostic Applications: Part I: Lipids and Fabrication Techniques

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