PLGA–mPEG nanoparticles of cisplatin: in vitro nanoparticle degradation, in vitro drug release and in vivo drug residence in blood properties

PLGA-Based Nanoparticles as Cancer Drug Delivery Systems

“…This is a widely used method to recognize the surface charges of NPs (Ratner et al, 1987;Soppimath et al, 2001). Hydrophobicity and hydrophilicity determined by Water contact angle measurements and hydrophobic interaction chromatography respectively (Storm et al, 1995;Manuela Gaspar et al, 1998;Mosqueira et al, 2001;Prior et al, 2002;Avgoustakis et al, 2002;. There are many sensitive methods for characterizing nanoparticles, depending upon the factor being investigated (Dinarvand et al, 2011).…”

“…This drug has been encapsulated on PLGA-mPEG nanoparticles prepared by double emulsion methods. (Avgoustakis et al, 2002;Kumari et al, 2010). Cisplatin-loaded PLGA-methoxy(polyethylene glycol) (mPEG) nanaoparticles also resulted in prolonged cisplatin residence time in the systemic circulation when used in mice with prostate tumor (Gryparis et al, 2007;Dinarvand et al, 2011).…”

“…Hydrophilic polymers can be useful at the surface of NPs by adsorption of surfactants or by utilize of block copolymers or branched copolymers (Stolnik et al, 1995;Storm et al, 1995;Torchilin and Trubetskoy, 1995;Mahapatro and Singh, 2011). Surface chemistry Analysis is determined by X-ray photoelectron spectroscopy (Mu and Feng, 2003;Si-Shen et al, 2004;Kim et al, 2005), Fourier transform infrared spectroscopy (Li et al, 2001;Choi and Kim, 2007;Yang et al, 2007) and Nuclear magnetic resonance spectroscopy (Li et al, 2001;Avgoustakis et al, 2002;Cheng et al, 2007;Dinarvand et al, 2011).…”

The role of PEG conformation in mixed layers: from protein corona substrate to steric stabilization avoiding protein adsorption

“…Pegylation of NPs has been proposed several times as a methodology to confer stability to quantum dots and AuNPs in biological media [29]. Additionally, it has been extensively SOR-MATSCI Joan Comenge and Víctor F. Puntes: Role of PEG conformation in mixed layers used to stealth polymeric nanostructures from being rapidly cleared by the immune system [8,[30][31][32], since it efficiently avoids opsonization (the process by which opsonins bind to the surface of foreign matter so that it will be readily identified and engulfed by phagocytes). First, we analyzed how the addition of different ratios of SH-PEG/MUA affects the loading of these molecules (Figure 2a).…”

“…In fact, the ability of engineering the physicochemical properties of these conjugates is important to tune biological responses such as cell uptake, protein adsorption or immunogenicity among others [6,7]. This is the case of stealthing with the FDA-approved polymer polyethylene glycol (PEG) [8][9][10]. After the simple, but widely exploited, use of PEG as surfactant for NPs (just by adding it in excess), many efforts are now being carried out unveiling the mechanism of action and to properly characterize these nanosystems to achieve a better control over physicochemical properties such as colloidal stability at working conditions or interactions with proteins [6,[10][11][12][13].…”