PLGA artificial nerve conduits with dental pulp cells promote facial nerve regeneration

Sasaki, Ryo; Uchiyama, Hiroto; Ogiuchi, Hideki; Ando, Tomohiro

doi:10.5794/jjoms.59.404

Cited by 34 publications

(49 citation statements)

References 36 publications

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“…The collagen-coated PLGA nerve conduit seeded with human nMSCs is an improvement in the development of a suitable conduit system for nerve regeneration. Previous studies have shown that PLGA-coated collagen is superior to vein graft in nerve regeneration [20,21]. Furthermore, the ability of human MSCs to differentiate into neuronal cells and facilitate the neuronal growth that has been studied previously [15,22].…”

Section: Discussionmentioning

confidence: 99%

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit

Sulong¹,

Hassan²,

Ng³

et al. 2014

Adv Clin Exp Med

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Background. Autologous nerve grafts to bridge nerve gaps pose various drawbacks. Nerve tissue engineering to promote nerve regeneration using artificial neural conduits has emerged as a promising alternative. Objectives. To develop an artificial nerve conduit using collagen-coated polylactic-glycolic acid (PLGA) and to analyse the survivability and propagating ability of the neuro-differentiated human mesenchymal stem cells in this conduit. Material and Methods. The PLGA conduit was constructed by dip-molding method and coated with collagen by immersing the conduit in collagen bath. The ultra structure of the conduits were examined before they were seeded with neural-differentiated human mesenchymal stem cells (nMSC) and implanted sub-muscularly on nude mice thighs. The non-collagen-coated PLGA conduit seeded with nMSC and non-seeded non-collagen-coated PLGA conduit were also implanted for comparison purposes. The survivability and propagation ability of nMSC was studied by histological and immunohistochemical analysis. Results. The collagen-coated conduits had a smooth inner wall and a highly porous outer wall. Conduits coated with collagen and seeded with nMSCs produced the most number of cells after 3 weeks. The best conduit based on the number of cells contained within it after 3 weeks was the collagen-coated PLGA conduit seeded with neuro-transdifferentiated cells. The collagen-coated PLGA conduit found to be suitable for attachment, survival and proliferation of the nMSC. Minimal cell infiltration was found in the implanted conduits where nearly all of the cells found in the cell seeded conduits are non-mouse origin and have neural cell markers, which exhibit the biocompatibility of the conduits. Conclusions. The collagen-coated PLGA conduit is biocompatible, non-cytotoxic and suitable for use as artificial nerve conduits (Adv Clin Exp Med 2014, 23, 3, 353-362).

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Section: Discussionmentioning

confidence: 99%

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit

Sulong¹,

Hassan²,

Ng³

et al. 2014

Adv Clin Exp Med

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“…Other sources of stem cells have been used in peripheral nerve injuries, such as adipose-derived stem cells [203,206,212,[226][227][228][229][230][231] and dental pulp stem cells (DPSCs) [232][233][234][235][236]. Dental pulp stem cells have also demonstrated differentiation capacity towards multiple other mesodermal and endodermal lineages, under appropriate conditions: adipogenic, osteo/dentinogenic, chondrogenic, neurogenic, endothelial, myofibroblastic [237] and hepatocytes [238].…”

Section: Cellular Systemsmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Human adult peripheral nerve injuries are a high incidence clinical problem that greatly affects patients' quality of life. Although peripheral nervous system has intrinsic regenerative capacity, this occurs in an incomplete or poorly functional manner. When a nerve fiber loses its continuity with consequent damage of the basal lamina tubes, axon spontaneous regeneration is disorganized and mismatched. These phenomena translate in an inadequate nerve functional recovery and consequent musculoskeletal incapacity. Nerve grafts still remain the gold standard in peripheral injuries treatment. However, this approach contains its disadvantages such as the necessity of primary surgery to harvest the autografts, loss of a functional nerve, donor site morbidity and longer surgery procedures. Therefore, biomaterials and tissue engineering can provide efficient resources and alternatives to nerve injury repair not only by the development of biocompatible structures but also, introducing neurotrophic factors and cellular systems to stimulate optimum clinical outcome. In this chapter, a comprehensive state-of-the art picture of tissue-engineered nerve grafts scaffolds, their application in nerve regeneration along with latest advances in peripheral nerve repair and future perspectives will be discussed, including our own large experience in this field of knowledge.

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“…Various types of transplanted stem cells were shown to enhance peripheral nerve regeneration in preclinical animal studies of nerve reconstruction. A variety of cell types, such as bone marrow stromal cells, adipose-derived stromal cells, muscle-derived stem cells, and dental pulp stem cells, were applied to this approach [17][18][19][20][21][22][23][24]. However, recent studies have reported low transplanted stem cell survival rates.…”

mentioning

confidence: 99%

Peripheral Nerve Regeneration by Secretomes of Stem Cells from Human Exfoliated Deciduous Teeth

Sugimura‐Wakayama

Katagiri

Osugi

et al. 2015

Stem Cells and Development

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Peripheral nerve regeneration across nerve gaps is often suboptimal, with poor functional recovery. Stem cell transplantation-based regenerative therapy is a promising approach for axon regeneration and functional recovery of peripheral nerve injury; however, the mechanisms remain controversial and unclear. Recent studies suggest that transplanted stem cells promote tissue regeneration through a paracrine mechanism. We investigated the effects of conditioned media derived from stem cells from human exfoliated deciduous teeth (SHED-CM) on peripheral nerve regeneration. In vitro, SHED-CM-treated Schwann cells exhibited significantly increased proliferation, migration, and the expression of neuron-, extracellular matrix (ECM)-, and angiogenesis-related genes. SHED-CM stimulated neuritogenesis of dorsal root ganglia and increased cell viability. Similarly, SHED-CM enhanced tube formation in an angiogenesis assay. In vivo, a 10-mm rat sciatic nerve gap model was bridged by silicon conduits containing SHED-CM or serum-free Dulbecco's modified Eagle's medium. Light and electron microscopy confirmed that the number of myelinated axons and axon-to-fiber ratio (G-ratio) were significantly higher in the SHED-CM group at 12 weeks after nerve transection surgery. The sciatic functional index (SFI) and gastrocnemius (target muscle) wet weight ratio demonstrated functional recovery. Increased compound muscle action potentials and increased SFI in the SHED-CM group suggested sciatic nerve reinnervation of the target muscle and improved functional recovery. We also observed reduced muscle atrophy in the SHED-CM group. Thus, SHEDs may secrete various trophic factors that enhance peripheral nerve regeneration through multiple mechanisms. SHED-CM may therefore provide a novel therapy that creates a more desirable extracellular microenvironment for peripheral nerve regeneration.

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PLGA artificial nerve conduits with dental pulp cells promote facial nerve regeneration

Cited by 34 publications

References 36 publications

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Peripheral Nerve Regeneration by Secretomes of Stem Cells from Human Exfoliated Deciduous Teeth

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