PLGA-Alendronate Conjugate as a New Biomaterial to Produce Osteotropic Drug Nanocarriers

Pignatello, Rosario

doi:10.5772/25766

Cited by 16 publications

(20 citation statements)

References 39 publications

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“…The gels with the highest turbidity, indicating zero charge of macromolecules, were obtained after 24h in the vials with pH value ranged from 4.7 to 4.9. These values correspond to IEP of the gelatine type "B", equal to 4.6…5.4, usually obtained by alkaline hydrolysis of skins [12].…”

Section: Experimental Part Preparation Of Gelatine Solutionsmentioning

confidence: 57%

“…Control experiments showed the greatest turbidity of pure gelatine solutions at pH 4.75-5.00. Therefore, the top point of this area corresponds to the isoelectric point of gelatine type "B" [12]. This fact explains nearly neutral charge of complexes GelAlg in the conditions, when m Gel /m Alg ratio tends to infi nite and pH is near IEP value.…”

Section: Phase Diagram Construction and Its Applications For Microcapmentioning

confidence: 86%

“…The study led to the elaboration of different phase diagrams, which refl ects complex interactions between the negatively charged polysaccharides and zwitterions of proteins [8,12,13]. The interaction of proteins with polysaccharides was described in numerous research papers, but not in the exhaustive form.…”

Section: Introductionmentioning

confidence: 99%

“…Such three-dimensional structures (3D) appear also during the complex coacervation of oppositely charged macromolecules on the oil droplets surface in the microencapsulation processes [11]. The optimum conditions of precipitation of alginate-gelatine coacervates were obtained in vitro, using measurements of turbidity and viscosity [1].The study led to the elaboration of different phase diagrams, which refl ects complex interactions between the negatively charged polysaccharides and zwitterions of proteins [8,12,13]. The interaction of proteins with polysaccharides was described in numerous research papers, but not in the exhaustive form.…”

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confidence: 99%

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Phase Diagram of Gelatine-Polyuronate Colloids: Its Application for Microencapsulation and Not Only

Baerle¹,

Dimova²,

Urumoglova³

et al. 2016

ChemJMold

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Abstract. Phase state and the charge of colloidal particles in the gelatine-polyuronate system were studied. A method for comparative evaluation of molecular weight of colloids by means of viscosimetric measurements and electrophoresis was developed. It is shown that the Diagram {Phase state = f (composition, pH)} contains six welldefi ned regions. The diagram explains and predicts the behaviour of protein-polysaccharide colloids, which are included in beverages or forms the shells of oil-containing microcapsules.Keywords: protein-polysaccharides, colloids, electrokinetic potential, phase diagram, microcapsules. IntroductionPoorly soluble or electro-statically agglomerated macromolecular compounds derive from interaction of protein zwitterions with polyanions, in particular, with polyuronic acids and their salts [1]. These interactions are of great practical interest, insofar as much of foods contain biopolymers in different combinations [2]. Recently, there were published many papers devoted to the joint use of the proteins and polysaccharides, originate from different natural raw materials, in various pharmaceutical and food systems [3][4][5][6]. These compounds have a greater ability to precipitate the colloidal suspensions, compared to pure linear polymers [7]. It is believed that these mixtures are in fact the covalent chemical compounds or supra-molecular three-dimensional structures with high molecular weight, charged as zwitterions due to the presence of proteins [8][9][10]. Due to this fact, the mixtures are capable to precipitate not only the neutral particles, but also charged particles, both positive and negative. Such three-dimensional structures (3D) appear also during the complex coacervation of oppositely charged macromolecules on the oil droplets surface in the microencapsulation processes [11]. The optimum conditions of precipitation of alginate-gelatine coacervates were obtained in vitro, using measurements of turbidity and viscosity [1].The study led to the elaboration of different phase diagrams, which refl ects complex interactions between the negatively charged polysaccharides and zwitterions of proteins [8,12,13]. The interaction of proteins with polysaccharides was described in numerous research papers, but not in the exhaustive form. Among the problematic issues it can be identifi ed the determination of the molecular weight of the resulting supramolecular structures. In our view, the estimation of the molecular weights will be useful for calculating of the microcapsule shells parameters, as well as to determine the conditions of deposition of neutral colloidal particles. Detailed analysis of the phase state of the gelatine-polyuronate system is important to determine the optimal sedimentation conditions, or opposite, to prevent the coagulation of proteins by small amounts of extraneous poly-anions. In constructing of the phase diagram of the gelatine-polyuronate, it is important to consider the sign and magnitude of charge of generated complexes. Unambiguous information about the char...

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Section: Experimental Part Preparation Of Gelatine Solutionsmentioning

confidence: 57%

Section: Phase Diagram Construction and Its Applications For Microcapmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Phase Diagram of Gelatine-Polyuronate Colloids: Its Application for Microencapsulation and Not Only

Baerle¹,

Dimova²,

Urumoglova³

et al. 2016

ChemJMold

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“…Compared with micro and macro-topography, surfaces with a more defined, reliable and reproducible nanostructure roughness provide a more conducive environment for osteoblast function in vivo. 1 Due to the advantageous high surface-to-volume ratio as well as higher degree of biocompatibility, titanium dioxide nanotubes (TNTs) are of particular interest. 2,3 Cell activity is directed by cell-implant surface interactions and a series of subsequent intracellular cascades would be triggered by the material topography.…”

Section: Introductionmentioning

confidence: 99%

Proliferation of preosteoblasts on TiO₂nanotubes is FAK/RhoA related

Yang

Nagasawa

et al. 2015

RSC Adv.

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Topographies promote surface-dependent behaviors which may positively influence peri-implant bone healing. In this study the topological effects of TiO2 nanotubes (TNTs) on aspects of preosteoblast behavior was investigated. Specifically, we hypothesize that TNTs can influence cell proliferation of preosteoblasts through cell adhesion and related modulation of FAK and RhoA. By culturing MC3T3-E1 cells on TNTs with different diameters (40nm and 150nm diameters), topography-dependent modulation in cell morphology and cell growth were observed. The average spreading area of the cell on Flat Ti, 40nm TNTs and 150nm TNTs were respectively 2176.05 μm2, 1510.44 μm2 and 800.72 μm2. Proliferation increased among cells cultured on the 150nm TNTs (28.6%) compared with on Flat Ti (17.06%). The expression of FAK was 86.2% down regulated superimposition of TNTs topography. RhoA expression only slightly decreased (45.9%). Increasing TNT diameter enhanced initial adherent cell growth, which was relevant to the increased RhoA-to-FAK ratio in the cell. Increased TNT diameter was associated with higher ratio and greater proliferation in the first 24 hours. These findings not only support our hypothesis, but suggest that RhoA might be critically involved in TNTs mediated cell proliferation. Future investigation using functional gain and loss of RhoA may further reveal its mechanism.

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Quantitation of native and forced degraded collagens by capillary zone electrophoresis: Method development and validation

Olabi,

Wätzig

2023

J of Separation Science

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A new capillary zone electrophoresis method for collagen quantitation was developed and validated according to the International Council for Harmonization guideline Q2 (R1). The Sircol collagen assay and ultraviolet spectrometry were employed as reference methods. Capillary zone electrophoresis enables specific, simple, and fast determination within 9 min. It is less user‐dependent and more automated than the Sircol collagen assay. With a limit of detection of 18.0 μg/mL, the new method is less sensitive than the Sircol collagen assay, which has a limit of detection of 6.5 μg/mL. Nonetheless, capillary zone electrophoresis covers a wider linearity range (50‐400 μg/mL) compared to the Sircol collagen assay (5‐80 μg/mL), with similar precision. Additional advantages of capillary zone electrophoresis are the ability to gain information on collagen integrity and to simultaneously determine native and denatured collagens. This approach represents a modern and legitimate alternative to the Sircol collagen assay. The developed method has been successfully applied to the study of three collagen products and samples from forced degradation.

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PLGA-Alendronate Conjugate as a New Biomaterial to Produce Osteotropic Drug Nanocarriers

Cited by 16 publications

References 39 publications

Phase Diagram of Gelatine-Polyuronate Colloids: Its Application for Microencapsulation and Not Only

Phase Diagram of Gelatine-Polyuronate Colloids: Its Application for Microencapsulation and Not Only

Proliferation of preosteoblasts on TiO₂nanotubes is FAK/RhoA related

Quantitation of native and forced degraded collagens by capillary zone electrophoresis: Method development and validation

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PLGA-Alendronate Conjugate as a New Biomaterial to Produce Osteotropic Drug Nanocarriers

Cited by 16 publications

References 39 publications

Phase Diagram of Gelatine-Polyuronate Colloids: Its Application for Microencapsulation and Not Only

Phase Diagram of Gelatine-Polyuronate Colloids: Its Application for Microencapsulation and Not Only

Proliferation of preosteoblasts on TiO2nanotubes is FAK/RhoA related

Quantitation of native and forced degraded collagens by capillary zone electrophoresis: Method development and validation

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Proliferation of preosteoblasts on TiO₂nanotubes is FAK/RhoA related