Pleural effusion osmolality correlation with pH and glucose level of pleural fluid and its effects on the pleural membrane permeability

Peppa, Vasiliki-Georgia; Solenov, Evgeniy I.; Kalomenidis, Ioannis; Tsilioni, Irene; Gourgoulianis, Konstantinos; Hatzoglou, Chrissi; Zarogiannis, Sotirios G.

doi:10.1016/j.resp.2020.103581

Cited by 4 publications

(2 citation statements)

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“…BBS2 and BBS18 gene expression were the most pronounced in all cell lines tested. Building on this finding future experiments will need to assess whether the gene expression of BBS2 and BBS18 changes under certain stimuli that are relevant to malignancy, such as oxidative stress, inflammation, and osmotic imbalance to name a few [ 38 – 40 ]. Furthermore, in the same line of thinking assessing how their gene expression patterns change under the influence of pleural effusions of different etiologies will provide more in-depth information on the role of BBSome in the context of pleural diseases in general.…”

Section: Discussionmentioning

confidence: 99%

Changes in expression of mesothelial BBS genes in 2D and 3D after lithium chloride and ammonium sulphate induction of primary cilium disturbance: a pilot study

et al. 2023

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Background Malignant pleural mesothelioma (MPM), a rare and aggressive pleural tumor, has significant histological and molecular heterogeneity. Primary Cilium (PC), an organelle of emerging importance in malignancies, has been scarcely investigated in MPM. A critical molecular complex for the PC function is the BBSome and here we aimed at assessing its expression patterns in ordinary 2D and spheroid 3D cell cultures. Methods A human benign mesothelial cell line (MeT-5A), MPM cell lines (M14K, epithelioid MPM; MSTO, biphasic MPM), and primary MPM cells (pMPM) were used. Primers specific for the human BBS1, 2, 4, 5, 7, 9, 18 transcripts were designed, and quantitative real-time PCR (qRT-PCR) was done with β-actin as the gene of reference. The relative gene expression across 2D and 3D cultures was analyzed by the expression factor (mean of 1/ΔCt values). With the 2–∆∆Ct method the gene expression fold changes were assessed from qRT-PCR data. Molecular changes using the PC-modulating drugs ammonium sulfate (AS) and lithium chloride (LC) were also determined. Results PC was present in all cells used in the study at approximately 15% of the observed area. BBSome transcripts were differentially expressed in different dimensions of cell culture (2D vs. 3D) in all cell lines and pMPM. Treatment with AS and LC affected the expression of the ciliary BBS2 and BBS18 genes in the benign as well as in the MPM cells. Conclusions These data indicate distinct BBSome molecular profiles in human benign and MPM cells cultured in 2D and 3D dimensions and support the notion that PC genes should be investigated as potential MPM therapeutic targets.

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Section: Discussionmentioning

confidence: 99%

Changes in expression of mesothelial BBS genes in 2D and 3D after lithium chloride and ammonium sulphate induction of primary cilium disturbance: a pilot study

et al. 2023

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“…The osmotic water permeability of the benign mesothelial cells was 0.005 cm/s which is much lower compared to OMCD principal cells, which have an osmotic water permeability of 0.12 cm/s [10]. In patients with pleural effusions of different etiologies, effusion osmolality ranges from 240 to 340 mOsm/kgr H 2 O. Pleural mesothelial cells in pathological conditions (pleural infection or malignancy) have to regulate their volume and adjust to aniso-osmolality [14]. Likewise, peritoneal mesothelial cells are also exposed to variable osmotic conditions with ascites formation.…”

Section: Introductionmentioning

confidence: 92%

Comparison of Isotonic Activation of Cell Volume Regulation in Rat Peritoneal Mesothelial Cells and in Kidney Outer Medullary Collecting Duct Principal Cells

et al. 2021

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In disease states, mesothelial cells are exposed to variable osmotic conditions, with high osmotic stress exerted by peritoneal dialysis (PD) fluids. They contain unphysiologically high concentrations of glucose and result in major peritoneal membrane transformation and PD function loss. The effects of isotonic entry of urea and myo-inositol in hypertonic (380 mOsm/kg) medium on the cell volume of primary cultures of rat peritoneal mesothelial cells and rat kidney outer medullary collecting duct (OMCD) principal cells were studied. In hypertonic medium, rat peritoneal mesothelial cells activated a different mechanism of cell volume regulation in the presence of isotonic urea (100 mM) in comparison to rat kidney OMCD principal cells. In kidney OMCD cells inflow of urea into the shrunken cell results in restoration of cell volume. In the shrunken peritoneal mesothelial cells, isotonic urea inflow caused a small volume increase and activated regulatory volume decrease (RVD). Isotonic myo-inositol activated RVD in hypertonic medium in both cell types. Isotonic application of both osmolytes caused a sharp increase of intracellular calcium both in peritoneal mesothelial cells and in kidney OMCD principal cells. In conclusion, peritoneal mesothelial cells exhibit RVD mechanisms when challenged with myo-inositol and urea under hyperosmolar isotonic switch from mannitol through involvement of calcium-dependent control. Myo-inositol effects were identical with the ones in OMCD principal cells whereas urea effects in OMCD principal cells led to no RVD induction.

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Mass transfer effects on mucus fluid in the presence of chemical reaction

Thiyagarajan

Sathiamoorthy

Balasundaram

et al. 2023

Alexandria Engineering Journal

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Pleural effusion osmolality correlation with pH and glucose level of pleural fluid and its effects on the pleural membrane permeability

Cited by 4 publications

References 9 publications

Changes in expression of mesothelial BBS genes in 2D and 3D after lithium chloride and ammonium sulphate induction of primary cilium disturbance: a pilot study

Changes in expression of mesothelial BBS genes in 2D and 3D after lithium chloride and ammonium sulphate induction of primary cilium disturbance: a pilot study

Comparison of Isotonic Activation of Cell Volume Regulation in Rat Peritoneal Mesothelial Cells and in Kidney Outer Medullary Collecting Duct Principal Cells

Mass transfer effects on mucus fluid in the presence of chemical reaction

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