PLETHORA transcription factors orchestrate de novo organ patterning during
            <i>Arabidopsis</i>
            lateral root outgrowth

Du, Yujuan; Scheres, Ben

doi:10.1073/pnas.1714410114

Cited by 103 publications

(122 citation statements)

References 48 publications

(84 reference statements)

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“…4B). PLT3, TCP20, and TCP21 fusion proteins were detected at stage I, whereas SCR expression was consistently observed in the stage II outer cell layer (Goh et al 2016;Du and Scheres 2017). Notably, WOX5 promoter activity was detected from stage II onward in cells where PLT, SCR, and TCP proteins are all expressed.…”

Section: A-1mentioning

confidence: 95%

“…, and plt1- roots (Bennett and Scheres 2010;Hofhuis et al 2013;Tian et al 2014;Goh et al 2016;Du and Scheres 2017). During lateral root initiation, two to three adjacent pericycle cell files undergo several rounds of anticlinal divisions to create a single layered stage I lateral root primordium (LRP) composed of up to eight to 10 cells of equal size.…”

Section: Tcp20-1mentioning

confidence: 99%

“…The stem cells, in turn, are pluripotent cells whose progeny normally generate defined cell types of the root depending on their position but reveal developmental plasticity upon perturbation (Xu et al 2006;Sena et al 2009). The entire root stem cell niche is first laid down in the embryo but can be re-established during lateral root formation or during regeneration upon damage (Malamy and Benfey 1997;Sena et al 2009;Efroni et al 2016;Du and Scheres 2017).…”

mentioning

confidence: 99%

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Root stem cell niche organizer specification by molecular convergence of PLETHORA and SCARECROW transcription factor modules

et al. 2018

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Continuous formation of somatic tissues in plants requires functional stem cell niches where undifferentiated cells are maintained. In Arabidopsis thaliana, PLETHORA (PLT) and SCARECROW (SCR) genes are outputs of apicalbasal and radial patterning systems, and both are required for root stem cell specification and maintenance. The WUSCHEL-RELATED HOMEOBOX 5 (WOX5) gene is specifically expressed in and required for functions of a small group of root stem cell organizer cells, also called the quiescent center (QC). PLT and SCR are required for QC function, and their expression overlaps in the QC; however, how they specify the organizer has remained unknown. We show that PLT and SCR genetically and physically interact with plant-specific teosinte-branched cycloidea PCNA (TCP) transcription factors to specify the stem cell niche during embryogenesis and maintain organizer cells post-embryonically. PLT-TCP-SCR complexes converge on PLT-binding sites in the WOX5 promoter to induce expression.

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Section: A-1mentioning

confidence: 95%

Section: Tcp20-1mentioning

confidence: 99%

mentioning

confidence: 99%

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Root stem cell niche organizer specification by molecular convergence of PLETHORA and SCARECROW transcription factor modules

et al. 2018

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“…We, therefore, next, investigated the genes regulated by OsWOX10 during CRP initiation and outgrowth. In rice, OsERF3 and OsCRL1 regulate CRP initiation, whereas Arabidopsis PLT genes functions during LR primordia outgrowth Zhao et al, 2015;Du and Scheres, 2017). Consistently, the expression of OsERF3 and OsCRL1 and their related genes (i.e.…”

Section: Arabidopsismentioning

confidence: 71%

Genome-Wide High Resolution Expression Map and Functions of Key Cell Fate Determinants Reveal the Dynamics of Crown Root Development in Rice

Garg

Singh

Chennakesavulu

et al. 2020

Preprint

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Rice adventitious/crown roots developing from non-root tissues shape up the root architecture.Mechanisms underlying initiation and subsequent outgrowth of CR primordia (CRP) remain under explored. Here, we provide genome-wide dynamics of gene expression patterns and stage-specific transcriptional signatures at distinct developmental stages of CRP formation. Our analyses reveal that early regulated transcription of potential epigenetic modifiers, transcription factors and cell division regulators prime the initiation of CRP followed by progressive activation of auxin signaling modules ensure their outgrowth. In depth analysis of spatio-temporal expression patterns of key cell fate determinants and functional analyses of rice WUSCHEL RELATED HOMEOBOX10 (OsWOX10) and PLETHORA (OsPLT) genes reveal their unprecedented role in CRP development. Our study suggests that OsWOX10 activates OsERF3 and OsCRL1 expression during CRP initiation and OsPLTs expression to accomplish their outgrowth. Interestingly, OsPLT genes, when expressed in the transcriptional domain of root-borne lateral root primordia of Arabidopsis plt mutant, rescued their outgrowth demonstrating the conserved role of PLT genes in root primordia outgrowth irrespective of their developmental origin. Together, these findings unveil the molecular framework of cellular reprogramming during trans-differentiation of shoot tissue to root leading to culmination of robust root architecture in monocot species which got evolutionary diverged from dicots. T.G. performed experiments of LCM, RNA in situ hybridization and OsWOX10 function in rice. Z.S. performed experiments for auxin responses and functions of OsPLT1 and OsPLT4 in rice. A.K.D., R.S.S and M.J. performed RNA sequencing data analysis. M.Y. with T.G. contributed for function of OsWOX10 in Arabidopsis. V.V. studied function of rice PLTs in Arabidopsis. D.C. contributed in LCM experiments. M.J., K.P. and S.R.Y. designed experiments, analyzed data and Academy of Sciences, Shanghai, China is acknowledged for kindly providing Arabidopsis wox11-2, wox12-1 and wox11-2 wox12-1 seeds. Ashish Kumar and Sonia Choudhary are acknowledged for their support in growing plants.

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“…During the course of LR development, specific modules of AUXIN RESPONSE FACTOR (ARF) transcription factors and AUXIN/INDOLE ACETIC ACID (AUX/IAA) repressor proteins, as well as other downstream transcription factors, specify LR primordium (LRP), founder cells and promote LR formation [37][38][39]. In parallel, three PLETHORA transcription family members-PLT3, PLT5, and PLT7-contribute to the establishment of LRPs [40]. During LR development, quiescent center (QC) is established by the action of a transcription factor complex comprising PLT, SCARECROW (SCR), and several members of TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) through activation of WUSCHEL-RELATED HOMEOBOX5 (WOX5) expression [41,42].…”

mentioning

confidence: 99%

The bRPS6-Family Protein RFC3 Prevents Interference by the Splicing Factor CFM3b during Plastid rRNA Biogenesis in Arabidopsis thaliana

Nagashima

Ohshiro

Iwase

et al. 2020

Plants

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Plastid ribosome biogenesis is important for plant growth and development. REGULATOR OF FATTY ACID COMPOSITION3 (RFC3) is a member of the bacterial ribosomal protein S6 family and is important for lateral root development. rfc3-2 dramatically reduces the plastid rRNA level and produces lateral roots that lack stem cells. In this study, we isolated a suppressor of rfc three2 (sprt2) mutant that enabled recovery of most rfc3 mutant phenotypes, including abnormal primary and lateral root development and reduced plastid rRNA level. Northern blotting showed that immature and mature plastid rRNA levels were reduced, with the exception of an early 23S rRNA intermediate, in rfc3-2 mutants. These changes were recovered in rfc3-2 sprt2-1 mutants, but a second defect in the processing of 16S rRNA appeared in this line. The results suggest that rfc3 mutants may be defective in at least two steps of plastid rRNA processing, one of which is specifically affected by the sprt2-1 mutation. sprt2-1 mutants had a mutation in CRM FAMILY MEMBER 3b (CFM3b), which encodes a plastid-localized splicing factor. A bimolecular fluorescence complementation (BiFC) assay suggested that RFC3 and SPRT2/CFM3b interact with each other in plastids. These results suggest that RFC3 suppresses the nonspecific action of SPRT2/CFM3b and improves the accuracy of plastid rRNA processing.

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PLETHORA transcription factors orchestrate de novo organ patterning during Arabidopsis lateral root outgrowth

Cited by 103 publications

References 48 publications

Root stem cell niche organizer specification by molecular convergence of PLETHORA and SCARECROW transcription factor modules

Root stem cell niche organizer specification by molecular convergence of PLETHORA and SCARECROW transcription factor modules

Genome-Wide High Resolution Expression Map and Functions of Key Cell Fate Determinants Reveal the Dynamics of Crown Root Development in Rice

The bRPS6-Family Protein RFC3 Prevents Interference by the Splicing Factor CFM3b during Plastid rRNA Biogenesis in Arabidopsis thaliana

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