Pleiotropy of pea RisfixC supernodulation mutation is symbiosis-independent

Novák, Karel; Lisá, Ludmila; Škrdleta, V.

doi:10.1007/s11104-010-0682-x

Cited by 4 publications

(4 citation statements)

References 53 publications

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“…Indeed, there is usually a trade-off for C use between roots and nodules to reach an optimal equilibrium between benefits related to SNF and C cost for nodule formation and functioning without impairing root development (Hacin et al, 1997;Tricot et al, 1997). In some cases, such as in hypernodulating mutants for which the autoregulation of the nodulation is disrupted (see the review from Mortier et al, 2012), many pleiotropic effects including shoot growth and yield depression have been reported (Novak et al, 2011). Our data suggest that a finely tuned nodule number initiation during the post-stress period is critical to ensure optimal N nutrition without excessive C costs (case of Kayanne), leading to plant growth recovery and contributing the N pool available for remobilization to the seeds later on during the reproductive period (Zeiher et al, 1982;Schiltz et al, 2005).…”

Section: Contrasted Strategies Between Genotypes During the Re-waterimentioning

confidence: 99%

Pea Efficiency of Post-drought Recovery Relies on the Strategy to Fine-Tune Nitrogen Nutrition

et al. 2020

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As drought is increasingly frequent in the context of climate change it is a major constraint for crop growth and yield. The ability of plants to maintain their yield in response to drought depends not only on their ability to tolerate drought, but also on their capacity to subsequently recover. Post-stress recovery can indeed be decisive for drought resilience and yield stability. Pea (Pisum sativum), as a legume, has the capacity to fix atmospheric nitrogen through its symbiotic interaction with soil bacteria within root nodules. Biological nitrogen fixation is highly sensitive to drought which can impact plant nitrogen nutrition and growth. Our study aimed at dynamically evaluating whether the control of plant N status after drought could affect nodulated pea plant's ability to recover. Two pea genotypes, Puget and Kayanne, displaying different drought resilience abilities were compared for their capacity to tolerate to, and to recover from, a 2-weeks water-deficit period applied before flowering. Physiological processes were studied in this time-series experiment using a conceptual structure-function analysis framework focusing on whole plant carbon, nitrogen, and water fluxes combined to two 13 CO 2 and 15 N 2 labeling experiments. While Puget showed a yield decrease compared to well-watered plants, Kayanne was able to maintain its yield. During the recovery period, genotype-dependent strategies were observed. The analysis of the synchronization of carbon, nitrogen, and water related traits dynamics during the recovery period and at the whole plant level, revealed that plant growth recovery was tightly linked to N nutrition. In Puget, the initiation of new nodules after water deficit was delayed compared to control plants, and additional nodules developed, while in Kayanne the formation of nodules was both rapidly and strictly re-adjusted to plant growth needs, allowing a full recovery. Our study suggested that a rapid re-launch of N acquisition, associated with a finetuning of nodule formation during the post-stress period is essential for efficient drought resilience in pea leading to yield stability.

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Section: Contrasted Strategies Between Genotypes During the Re-waterimentioning

confidence: 99%

Pea Efficiency of Post-drought Recovery Relies on the Strategy to Fine-Tune Nitrogen Nutrition

et al. 2020

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“…Nevertheless, only sparse information is available on the impact of hypernodulation on nitrogen nutrition in relation with carbon nutrition traits. In a few studies, hypernodulation was associated with higher N content in root tissues (Day et al 1986;Novak et al 2011), in the shoot (Novak et al 2011 and references therein) or in harvested seeds ), but this was not related to carbon nutrition traits. In other studies, hypernodulation was associated to lower specific N 2 fixing activity of nodules (in g N 2 fixed per g nodule) and high N status as compared to wild type , without full characterisation of carbon nutrition traits.…”

Section: Introductionmentioning

confidence: 98%

“…Hence, most studies on hypernodulating mutants have reported negative pleiotropic traits related to carbon nutrition such as depressed shoot growth, shortened internodes or fasciation of the stem, and reduction of roots development and growth (e.g., pea : Postma et al 1988;Duc and Messager 1989;Sagan and Duc 1996;Krusell et al 2002;Bourion et al 2007; soybean: Matsunami et al 2004;Voisin et al 2013). These pleiotropic effects might be a direct effect of the mutated gene on those carbon nutrition traits, or it may result from a physiological consequence of deregulated (and therefore excessive) nodule number (Novak et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Pea nodule gradients explain N nutrition and limited symbiotic fixation in hypernodulating mutants

Voisin

Prudent

Duc

et al. 2015

Agron. Sustain. Dev.

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Legumes fix atmospheric nitrogen by symbiosis with soil bacteria in root nodules. Legume yields are limited by the low capacity of N 2 fixation. Hypernodulating mutants have been selected decades ago to try to increase nodule number. However, literature data show that N fixation of hypernodulating mutants was not increased compared to parental lines. Here, we study the functional basis of limited N fixation associated to hypernodulation. We grew two wild type genotypes and nine hypernodulating mutants of pea in hydroponics in three greenhouse experiments. We measured the following traits related to N nutrition during the vegetative period: nodule number, plant N uptake, nodule-specific activity, and plant and nodule concentrations. Genetic and environmental variations induced nodule gradients. These gradients were used to set quantitative relationships between N nutrition traits and nodule number. We compared the relationships obtained for hypernodulating and for wild types. N nutrition traits were analysed together with C nutrition traits, through correlation networks. Our results show that higher nodule number of hypernodulating mutants is correlated with lower levels of nodule activity, from −25 to −60 %, by comparison to the wild type. Higher nodule number of hypernodulating mutants is also correlated with lower total N uptake by symbiotic fixation, from −0 to −60 %, by comparison to the wild type. Findings demonstrate that N nutrition is not a factor limiting growth in hypernodulating mutants, as shown by N nutrition index higher than 1, indicating N nutrition in excess. The correlations suggest that limited N 2 fixation in hypernodulating mutants arises from restricted shoot growth, which limits the plant capacity to accumulate N. Furthermore, symbiotic efficiency decreased with increasing nodule number, down to a minimal value for hypernodulating mutants. Thus, to overcome the trade-off between N benefits from N 2 fixation and carbon nodulation costs, the hypernodulation trait should be associated with high shoot growth capacity in breeding programs.

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“…Albeit without ultimate proof, these nodulation-related CLE peptides have been hypothesized to act as the Q signal to activate the CLV-like receptor complex in the shoot for AON. In addition to the CLE peptides, several mutants have been identified that might be affected in genes involved in the control of AON in the root, such as nod3, rdn1, rdh1, tml, and plenty (Postma et al, 1988;Ishikawa et al, 2008;Magori et al, 2009;Novák, 2010;Yoshida et al, 2010;Novák et al, 2011;Schnabel et al, 2011). These mutants might be defective in the genes that control the root-derived signals or the root-to-shoot processing/transducing signals or vice versa (Li et al, 2009;Novák, 2010).…”

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confidence: 99%

WUSCHEL-RELATED HOMEOBOX5Gene Expression and Interaction of CLE Peptides with Components of the Systemic Control Add Two Pieces to the Puzzle of Autoregulation of Nodulation

et al. 2012

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In legumes, the symbiotic nodules are formed as a result of dedifferentiation and reactivation of cortical root cells. A shootacting receptor complex, similar to the Arabidopsis (Arabidopsis thaliana) CLAVATA1 (CLV1)/CLV2 receptor, regulating development of the shoot apical meristem, is involved in autoregulation of nodulation (AON), a mechanism that systemically controls nodule number. The targets of CLV1/CLV2 in the shoot apical meristem, the WUSCHEL (WUS)-RELATED HOMEOBOX (WOX) family transcription factors, have been proposed to be important regulators of apical meristem maintenance and to be expressed in apical meristem "organizers." Here, we focus on the role of the WOX5 transcription factor upon nodulation in Medicago truncatula and pea (Pisum sativum) that form indeterminate nodules. Analysis of temporal WOX5 expression during nodulation with quantitative reverse transcription-polymerase chain reaction and promoter-reporter fusion revealed that the WOX5 gene was expressed during nodule organogenesis, suggesting that WOX genes are common regulators of cell proliferation in different systems. Furthermore, in nodules of supernodulating mutants, defective in AON, WOX5 expression was higher than that in wild-type nodules. Hence, a conserved WUS/WOX-CLV regulatory system might control cell proliferation and differentiation not only in the root and shoot apical meristems but also in nodule meristems. In addition, the link between nodule-derived CLE peptides activating AON in different legumes and components of the AON system was investigated. We demonstrate that the identified AON component, NODULATION3 of pea, might act downstream from or beside the CLE peptides during AON.Legume plants can thrive on nitrogen-poor soils because they interact symbiotically with soil-resident bacteria, the rhizobia. After a complex signal exchange between both partners, new root organs are formed: the nodules, in which the bacteria fix atmospheric nitrogen for the plant in return for a protective niche and carbon sources. Nodulation requires well-controlled bacterial invasion and initiation of cortical cell division after perception of the bacterially produced Nodulation (Nod) factors. Studies in several legumes have elucidated many elements of the signaling cascade (Catoira et al.,

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Pleiotropy of pea RisfixC supernodulation mutation is symbiosis-independent

Cited by 4 publications

References 53 publications

Pea Efficiency of Post-drought Recovery Relies on the Strategy to Fine-Tune Nitrogen Nutrition

Pea Efficiency of Post-drought Recovery Relies on the Strategy to Fine-Tune Nitrogen Nutrition

Pea nodule gradients explain N nutrition and limited symbiotic fixation in hypernodulating mutants

WUSCHEL-RELATED HOMEOBOX5Gene Expression and Interaction of CLE Peptides with Components of the Systemic Control Add Two Pieces to the Puzzle of Autoregulation of Nodulation

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