Pleiotropic effect of the insertion of the Agrobacterium rhizogenes rolD gene in tomato (Lycopersicon esculentum Mill.)

Bettini, P.; Michelotti, S.; Bindi, Daniela; Giannini, Raffaello; Capuana, M.; Buiatti, M.

doi:10.1007/s00122-003-1322-0

Cited by 54 publications

(40 citation statements)

References 36 publications

(29 reference statements)

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“…The T-DNA (TL-and TR-DNA), the virulence (vir) genes, and the chromosomal virulence (chv) genes are the 3 groups of gene families necessary for DNA transfer from A. rhizogenes to plant cells. The 4 rol genes (root loci), rolA, rolB, rolC, and rolD, are carried by TL-DNA, which differentiates the Ri plasmid from the Ti plasmid of A. tumefaciens (Bettini et al, 2003;Chavarri et al, 2010). The func-tion of the rolB is indoxyl-β-oxidase activity and it plays a role in the secretion of active auxin (Altamura, 2003).…”

Section: Resultsmentioning

confidence: 99%

Improvement of atropine production by different biotic and abiotic elicitors in hairy root cultures of Datura metel

Shakeran¹,

Keyhanfar²,

Asghari³

et al. 2015

Turk J Biol

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Hairy roots induced by Agrobacterium rhizogenes are distinguished by their high growth rate, genetic stability, and lateral branching. Datura metel is an important medicinal plant whose hairy roots are used for the production of atropine, a tropane alkaloid applied as an anticholinergic and parasympathetic agent. To increase the yield of atropine, biotic (Bacillus cereus and Staphylococcus aureus) and abiotic (AgNO 3 and nanosilver) elicitors were added to the hairy root cultures of D. metel and then analyzed through highperformance liquid chromatography. All the elicitors influenced biomass accumulation and atropine production. Among the tested elicitors, nanosilver was most effective in enhancing the hairy roots' atropine content. To the authors' knowledge, the application of nanosilver for increasing tropane alkaloid production is reported for the first time here and could improve the atropine production of pharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

Improvement of atropine production by different biotic and abiotic elicitors in hairy root cultures of Datura metel

Shakeran¹,

Keyhanfar²,

Asghari³

et al. 2015

Turk J Biol

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“…A portion of Arabidopsis plants ectopically expressing P5CS1 were shorter in size and produced many coflourescences (clusters of flowers), had an overall "bushy" appearance because of reduced elongation of the main inflourescence stem, and had an extended lifespan compared to wild type (Mattioli et al, 2008). Overexpression of A. rhizogenes rolD, which encodes an OCD, also induced early flowering in transgenic tobacco (Mauro et al, 1996) and tomato (Bettini et al, 2003). This would suggest that increased proline can cause early flowering no matter which pathway of proline synthesis is used.…”

Section: Developmentmentioning

confidence: 99%

Proline Metabolism and Its Implications for Plant-Environment Interaction

Verslues

Sharma

2010

The Arabidopsis Book

440

347

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Proline has long been known to accumulate in plants experiencing water limitation and this has driven studies of proline as a beneficial solute allowing plants to increase cellular osmolarity during water limitation. Proline metabolism also has roles in redox buffering and energy transfer and is involved in plant pathogen interaction and programmed cell death. Some of these unique roles of proline depend on the properties of proline itself, whereas others depend on the "proline cycle" of coordinated proline synthesis in the chloroplast and cytoplasm with proline catabolism in the mitochondria. The regulatory mechanisms controlling proline metabolism, intercellular and intracellular transport and connections of proline to other metabolic pathways are all important to the in vivo functions of proline metabolism. Connections of proline metabolism to the oxidative pentose phosphate pathway and glutamate-glutamine metabolism are of particular interest. The N-acetyl glutamate pathway can also produce ornithine and, potentially, proline but its role and activity are unclear. Use of model systems such as Arabidopsis thaliana to better understand both these long studied and newly emerging functions of proline can help in the design of nextgeneration experiments testing whether proline metabolism is a promising metabolic engineering target for improving stress resistance of economically important plants.

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“…For instance, a constitutively modified endogenous hormonal background has been shown to affect the active defence response to fungal pathogens in tomato plants transgenic for the Agrobacterium rhizogenes rol genes (Bettini et al, 2003(Bettini et al, , 2010 and in rol-transformed cells of various Solanaceae, Araliaceae, Rubiaceae, Vitaceae and Rosaceae. In fact, the modified hormonal profile upregulates the activation of secondary metabolites involved in stress responses (Bulgakov, 2008).…”

Section: Introductionmentioning

confidence: 99%

Response to metal stress of Nicotiana langsdorffii plants wild-type and transgenic for the rat glucocorticoid receptor gene

Fuoco

Bogani

Capodaglio

et al. 2013

Journal of Plant Physiology

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a b s t r a c tRecently our findings have shown that the integration of the gene coding for the rat gluco-corticoid receptor (GR receptor) in Nicotiana langsdorffii plants induced morphophysiological effects in transgenic plants through the modification of their hormonal pattern. Phytohormones play a key role in plant responses to many different biotic and abiotic stresses since a modified hormonal profile up-regulates the activation of secondary metabolites involved in the response to stress. In this work transgenic GR plants and isogenic wild type genotypes were exposed to metal stress by treating them with 30 ppm cadmium(II) or 50 ppm chromium(VI). Hormonal patterns along with changes in key response related metabolites were then monitored and compared. Heavy metal up-take was found to be lower in the GR plants. The transgenic plants exhibited higher values of S-abscisic acid (S-ABA) and 3-indole acetic acid (IAA), salicylic acid and total polyphenols, chlorogenic acid and antiradical activity, compared to the untransformed wild type plants. Both Cd and Cr treatments led to an increase in hormone concentrations and secondary metabolites only in wild type plants. Analysis of the results suggests that the stress responses due to changes in the plant's hormonal system may derive from the interaction between the GR receptor and phytosteroids, which are known to play a key role in plant physiology and development.

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Pleiotropic effect of the insertion of the Agrobacterium rhizogenes rolD gene in tomato (Lycopersicon esculentum Mill.)

Cited by 54 publications

References 36 publications

Improvement of atropine production by different biotic and abiotic elicitors in hairy root cultures of Datura metel

Improvement of atropine production by different biotic and abiotic elicitors in hairy root cultures of Datura metel

Proline Metabolism and Its Implications for Plant-Environment Interaction

Response to metal stress of Nicotiana langsdorffii plants wild-type and transgenic for the rat glucocorticoid receptor gene

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