Pleiotropic control of glucose and hormone responses by PRL1, a nuclear WD protein, in <i>Arabidopsis</i>

Németh, Krisztián; Salchert, Klaus; Putnoky, Péter; Bhalerao, Rishikesh P.; Koncz-Kálmán, Zsuzsanna; Stangeland, Biljana; Bako, L.; Mathur, Jaideep; Ökrész, László; Stabel, Sylvia; Geigenberger, Peter; Stitt, Mark; Rédei, George P.; Schell, Jeff; Koncz, Csaba

doi:10.1101/gad.12.19.3059

Cited by 238 publications

(273 citation statements)

References 59 publications

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“…Although subsequent studies did not confirm in vivo formation of a CRK5-PRL1 complex under normal growth conditions, we found that inactivation of the CRK5 gene by a T-DNA insertion in the MPIZ mutant line 38225 (crk5-1; Ríos et al, 2002; see Supplemental Figure 1A online) resulted in reduced primary root elongation ( Figure 1A), analogously to the prl1 mutation (Németh et al, 1998). By contrast, a second T-DNA insertion in the CRK5 gene (Salk_003774, crk5-2) obtained from the SIGnAL collection (Alonso et al, 2003) failed to confer a root elongation detect.…”

Section: Inactivation Of Crk5 Results In Delayed Gravitropic Responsesmentioning

confidence: 84%

“…CRK5 (called alternatively PIP-D) was originally identified in yeast two-hybrid and in vitro protein binding assays as a potential interaction partner of the C-terminal WD40-repeat domain of nuclear PRL1 protein, a subunit of the spliceosome-activating complex (Németh et al, 1998;Koncz et al, 2012). Although subsequent studies did not confirm in vivo formation of a CRK5-PRL1 complex under normal growth conditions, we found that inactivation of the CRK5 gene by a T-DNA insertion in the MPIZ mutant line 38225 (crk5-1; Ríos et al, 2002; see Supplemental Figure 1A online) resulted in reduced primary root elongation ( Figure 1A), analogously to the prl1 mutation (Németh et al, 1998).…”

Section: Inactivation Of Crk5 Results In Delayed Gravitropic Responsesmentioning

confidence: 99%

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Inactivation of Plasma Membrane–Localized CDPK-RELATED KINASE5 Decelerates PIN2 Exocytosis and Root Gravitropic Response inArabidopsis

et al. 2013

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CRK5 is a member of the Arabidopsis thaliana Ca 2+ /calmodulin-dependent kinase-related kinase family. Here, we show that inactivation of CRK5 inhibits primary root elongation and delays gravitropic bending of shoots and roots. Reduced activity of the auxin-induced DR5-green fluorescent protein reporter suggests that auxin is depleted from crk5 root tips. However, no tip collapse is observed and the transcription of genes for auxin biosynthesis, AUXIN TRANSPORTER/AUXIN TRANSPORTER-LIKE PROTEIN (AUX/LAX) auxin influx, and PIN-FORMED (PIN) efflux carriers is unaffected by the crk5 mutation. Whereas AUX1, PIN1, PIN3, PIN4, and PIN7 display normal localization, PIN2 is depleted from apical membranes of epidermal cells and shows basal to apical relocalization in the cortex of the crk5 root transition zone. This, together with an increase in the number of crk5 lateral root primordia, suggests facilitated auxin efflux through the cortex toward the elongation zone. CRK5 is a plasma membrane-associated kinase that forms U-shaped patterns facing outer lateral walls of epidermis and cortex cells. Brefeldin inhibition of exocytosis stimulates CRK5 internalization into brefeldin bodies. CRK5 phosphorylates the hydrophilic loop of PIN2 in vitro, and PIN2 shows accelerated accumulation in brefeldin bodies in the crk5 mutant. Delayed gravitropic response of the crk5 mutant thus likely reflects defective phosphorylation of PIN2 and deceleration of its brefeldin-sensitive membrane recycling.

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Section: Inactivation Of Crk5 Results In Delayed Gravitropic Responsesmentioning

confidence: 84%

Section: Inactivation Of Crk5 Results In Delayed Gravitropic Responsesmentioning

confidence: 99%

Inactivation of Plasma Membrane–Localized CDPK-RELATED KINASE5 Decelerates PIN2 Exocytosis and Root Gravitropic Response inArabidopsis

et al. 2013

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“…The PRL1 gene codes for a WD-protein that, by analogy to COP1, functions as a negative regulator of a set of light-, hormone-, and stress-regulated genes (Ne¨meth et al 1998). Mutation of PLR1 results in complex phenotypic defects, including the inhibition of hypocotyl elongation in the dark.…”

Section: The Cpd Gene Is Regulated By Prl1 In Arabidopsismentioning

confidence: 99%

“…Genetic screening for PRL1 interacting proteins in the yeast two-hybrid system revealed that PRL1 speci¢cally binds a novel a-importin, ATHKAP2 (Ne¨meth et al 1998). ATHKAP2 does not bind to proteins containing monopartite and bipartite nuclear localization signals (NLS).…”

Section: Prl1 Is a Conserved A-importin-binding Nuclear Protein Thatmentioning

confidence: 99%

Control of cell elongation and stress responses by steroid hormones and carbon catabolic repression in plants

Salchert¹,

Bhalerao²,

Koncz-Kálmán³

et al. 1998

Phil. Trans. R. Soc. Lond. B

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Molecular analysis of Arabidopsis mutants displaying hypocotyl elongation defects in both the dark and light revealed recently that steroids play an essential role as hormones in plants. De¢ciencies in brassinosteroid biosynthesis and signalling permit photomorphogenic development and light-regulated gene expression in the dark, and result in severe dwar¢sm, male sterility and de-repression of stress-induced genes in the light. A cytochrome P450 steroid hydroxylase (CYP90) controls a rate limiting step in brassinosteroid biosynthesis and appears to function as a signalling factor in stress responses. Another key step in steroid biosynthesis is controlled by the Arabidopsis SNF1 kinases that phosphorylate the 3-hydroxy3methylglutaryl-CoA reductase. The activity of SNF1 kinases is regulated by PRL1, an evolutionarily conserved a-importin-binding nuclear WD-protein. The prl1 mutation results in cell elongation defects, de-repression of numerous stress-induced genes, and augments the sensitivity of plants to glucose, cold stress and several hormones, including cytokinin, ethylene, auxin, and abscisic acid.

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“…Transformation of plasmids into yeast was done according to the LiAc transformation method [19]. STK01 and STK03 constructs were cloned into the bait vector pAS2.1 (Clontech), and yeast-2-hybrid screening of an Arabidopsis cell suspension culture cDNA library in pACT2.1 [20] was carried out according to [21].…”

Section: Yeast Two-hybrid Screeningmentioning

confidence: 99%

Increased vegetative development and sturdiness of storekeeper-transgenic tobacco

Bömer¹,

Uhrig

Jach³

et al. 2011

Open Life Sciences

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The STOREKEEPER (STK) family of DNA-binding proteins work as transcription factors and the ectopic expression of two stk-like genes from Arabidopsis thaliana, stk01 (At1g61730) and stk03 (At4g00238), in tobacco increased the number of vegetative internodes and promoted plant and leaf size, stem diameter and sturdiness. The development of these plants started with rosette formation while pronounced shoot elongation and flowering was delayed. Moreover, when the STK01 and STK03 proteins were fused to the Herpes Simplex Virus VP16 transcriptional activation domain and expressed in tobacco the vigorous storekeeper-phenotype did not appear indicating that transgenic STK-like proteins in part worked as repressors of tobacco reproductive development. Furthermore, Yeast Two-Hybrid screenings proved that STK01 and STK03 can form homodimers and heterodimers with further members of the STKlike family. Therefore, we assume that interactions between transgenic Arabidopsis STKs and re sident tobacco STKs could have contributed to the observed developmental changes in transgenic tobacco. Our findings open up promising applications for overexpression of stk-like genes in crops that benefit from increased sturdiness and vegetative organ development, such as tobacco in molecular farming approaches, biomass-based energy crops and medicinal plants that produce bioactive compounds in leaves

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Pleiotropic control of glucose and hormone responses by PRL1, a nuclear WD protein, in Arabidopsis

Cited by 238 publications

References 59 publications

Inactivation of Plasma Membrane–Localized CDPK-RELATED KINASE5 Decelerates PIN2 Exocytosis and Root Gravitropic Response inArabidopsis

Inactivation of Plasma Membrane–Localized CDPK-RELATED KINASE5 Decelerates PIN2 Exocytosis and Root Gravitropic Response inArabidopsis

Control of cell elongation and stress responses by steroid hormones and carbon catabolic repression in plants

Increased vegetative development and sturdiness of storekeeper-transgenic tobacco

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