PLCE1 is a poor prognostic marker and may promote immune escape from osteosarcoma by the CD70-CD27 signaling pathway

Abstract

Phospholipase C epsilon 1 (PLCE1) is involved in the pathogenesis of many cancers. However, the biological role of PLCE1 in osteosarcoma (OS) is still poorly understood. The prognostic survival analysis was performed on the PLCE1gene in the TARGET data set and the differential expression of PLCE1 in OS tissue and normal bone tissue on the tissue chip was detected by immunohistochemistry. Spearman’s rank correlation coefficient analysis was implemented to explore the relationship between PLCE1 and immune genes. Finally, PLCE1 was silenced to explore its biological function in OS cells. The results of tissue chip immunohistochemistry showed that PLCE1 expression in OS tissue was higher than in normal bone tissue. The survival curve of PLCE1 and its corresponding receiver operating characteristic curve (ROC) showed that PLCE1 had a significant effect on the survival status of patients with OS and that the prognosis of patients with high PLCE1 expression was relatively poor. Spearman’s rank correlation coefficient analysis and qRT-PCR assays found that PLCE1 may promote immune escape from OS via CD70-CD27 signaling pathway. Silencing of PLCE1 causes the following biological behaviors of OS cells: it promotes apoptosis, inhibits proliferation of OS cells, and inhibits the ability of cell migration and invasion. PLCE1 is a poor prognostic marker and a potential key factor affecting the immune status of the OS tumor microenvironment.

Identification of candidate genes for feed efficiency and growth traits in Wenchang chickens

“…As the effector of heterotrimer and small G protein, PLCE1 is involved in regulating cell growth, T-cell activation, actin organization and cell survival. The GWAS results of the mapping to the PLCE1 gene function were mostly related to nervous system activity, which regulates the function of the brain in different ways, and the brain was key to regulating diet behavior and body energy homeostasis [36][37][38][39]. We showed that the candidate genes identified were key and might affect the feed efficiency of Wenchang chickens, and the gene expression level in the high and low RFI groups of broilers also validated this result.…”

Jun-APOE-LRP1 axis promotes tumor metastasis in colorectal cancer

“…As a multistage and multifactorial process, CRC metastasis remains a major cause of high mortality and poor prognosis. Recent studies have reported that abnormal lipid metabolism is involved in the progression of CRC, and many genes related to lipid metabolism have been found to play an oncogenic role [2][3][4][5][6]. Apolipoprotein E (apoE) is a secreted protein involved in lipid metabolism and in the development of Alzheimer's disease and atherosclerosis [7,8].…”

Genome-wide association analysis identify candidate genes for feed efficiency and growth traits in Wenchang chickens

“…As the effector of heterotrimer and small G protein, PLCE1 is involved in regulating cell growth, T-cell activation, actin organization and cell survival. Mapping the PLCE1 gene function were mostly related to nervous system activity, which regulates the function of the brain in different ways, and the brain was key to regulating diet behavior and body energy homeostasis [ 43 – 46 ].…”