PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells

Abstract

Abstract. Phospholipase C (PLC) is a pivotal enzyme in the phosphoinositide pathway that promotes the second messengers, diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), to participate in eukaryotic signal transduction. Several PLC isozymes are associated with cancer, such as PLC-β1, PLC-δ1, PLC-ε and PLC-γ1. However, the role of PLC-δ3 (PLCD3) in nasopharyngeal carcinoma (NPC) has not been investigated to date. In our previous study, we demonstrated that flotillin2 (Flot2) plays a pro-neoplastic role in NPC and is involved in tumour progression and metastasis. In the present study, we screened the interacting proteins of Flot2 using the yeast two-hybrid (Y2H) method and verified the interaction between PLCD3 and Flot2 by co-immunoprecipitation. We also investigated the biological functions of PLCD3 in NPC. Inhibition of PLCD3 expression impaired the malignant potential of 5-8F, a highly metastatic NPC cell line, by restraining its growth, proliferation, mobility and migration. The present study demonstrated that PLCD3 may be an oncogenic protein in NPC and that it plays an important role in the progression of NPC partially by interacting with Flot2.

Comprehensive transcriptome analysis of cerebral cavernous malformation across multiple species and genotypes

“…The 5 DEGs (SPARCL1, PLCD3, PDGFRA, PLXDC2, and FAXC) that were common among human lesional NVUs and the Ccm1/Krit1 genotypes of both BMECs and C. elegans do not currently have postulated functions in CCM disease. However, SPARCL1 and PLCD3 have been associated with cellular proliferation, migration, and cancer (29,30). PLCD3 belongs to a phospholipase C family and may be linked to the phosphatidylinositol signaling pathway (30).…”

“…However, SPARCL1 and PLCD3 have been associated with cellular proliferation, migration, and cancer (29,30). PLCD3 belongs to a phospholipase C family and may be linked to the phosphatidylinositol signaling pathway (30). PDGFRA is a growth factor also related to cellular growth, differentiation, and tumor vasculogenesis (31).…”

Phosphoinositide-Dependent Signaling in Cancer: A Focus on Phospholipase C Isozymes

“…PLCδ plays several essential roles in different tissue types. In particular, PLCδ1 is important for skin homeostasis and for tissue metabolism, while PLCδ3 promotes microvilli formation in enterocytes and the radial migration of neurons in the cerebral cortex during brain growth [13,51].…”

Advances in targeted therapy mainly based on signal pathways for nasopharyngeal carcinoma

“… 88 Besides, the important role of Flot-2 in the progression of NPC might be partially linked to its interaction with PLC-δ3 (PLCD3). 89 In addition, as a type I transmembrane glycoprotein, the role of epithelial cell adhesion molecule (EpCAM) in different cancers is distinct which might depend on the cell type and microenvironment. In head and neck squamous cell carcinomas (HNSCCs), the extracellular domain of EpCAM (EpEX) has a role of a ligand of EGFR that induces EGFR-dependent proliferation but counteracts EGF-induced EMT.…”