Platonin, a photosensitive cyanine dye as an immunomudulator

Polyarteritis nodosa (PN), characterized by vasculitis of medium and small sized arteries, is uncommon in children. This disease is thought to be concerned with some abnormal immune responses. Platonin (4,4'-dimethyl-3,3'-di-n-heptyl-8-[2-(4-methyl-3-n-heptylthiazole) ]-2,2'- dicarbocyanine diiodine) is one of the photosensitive dyes of trithiazole pentamethine cyanine. It has been reported that platonin stimulated suppressor/cytotoxic T cells, but suppressed B cells. In clinical observations, platonin is shown to be efficacious for rheumatoid arthritis and juvenile rheumatoid arthritis. In this study, a Japanese boy (9-year-old) with PN was given platonin in combination with prednisolone. Prednisolone was gradually tapered under a cover of platonin. As a result, the relapse has not been exhibited for a long time (72 months). There were no adverse side effects during long term administration of medication.

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Development of Enzyme Immunoassay for Platonin® (NK 19)

Yamamoto

Morishita

1986

Journal of Immunoassay

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A sensitive and specific enzyme immunoassay for an immunomodulator PLATONIN (NK 19) has been developed and applied to the evaluation of pharmacokinetics in human and rats. NK 19 analog, succinyl NK 19 has been synthesized and coupled to bovine serum albumin. This conjugate is immunogenic and when injected into rabbits antibodies of high affinity for NK 19 were obtained. Some light decomposed products of Platonin were of lower cross-reactivity with these antibodies. For competitive reactions, these antibodies were incubated with a mixture of succinyl NK 2900-beta-D-galactosidase conjugate and standard NK 19 or sample. Then, antibody-bound enzyme-hapten were separated from free one using anti-rabbit IgG immobilized on polystyrene balls. Activity of the enzyme on the solid phase was fluorometrically determined. The present immunoassay allows detection of as low as 10 pg of NK 19 in the biological fluids using 50 microliters of the samples. The accuracy and reproducibility were also quite satisfactory. By use of this assay, plasma levels of NK 19 after a single oral administration of 5 mg or 10 mg/body to human volunteers or intravenous injection of 2 micrograms/kg to rats could be determined.

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Platonin, a photosensitive cyanine dye as an immunomudulator

Cited by 5 publications

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Biologically Active Cyanine Dyes as Probes for the Identification of Active Oxygen Species

Biologically Active Cyanine Dyes as Probes for the Identification of Active Oxygen Species

A case report of a 9-year old boy with polyarteritis nodosa in which a combination therapy of corticosteroids and a photosensitive dye Platonin was effective

Development of Enzyme Immunoassay for Platonin® (NK 19)

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