Plating Medium for Differentiating
            <i>Salmonella arizonae</i>
            from Other Salmonellae

Littell, Allan M.

doi:10.1128/aem.33.2.485-487.1977

Cited by 6 publications

(6 citation statements)

References 4 publications

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“…Arizona colonies on BS agar have been reported as looking like typical Salmonella spp. colonies (13,16); however, like Greenfield et al (10), we found Arizona colonies on BS to be coliform-like, being olive green with darker centers. In addition, one strain of Arizona sp.…”

Section: Discussionsupporting

confidence: 73%

“…Reliance on such differentiation can be a problem in detecting salmonellae under certain instances where (i) the high acidity produced by the background colonies can mask the differential reaction of Salmonella colonies (18), and (ii) certain strains of salmonellae are able to utilize these sugars and subsequently resemble the background coliform organisms (2,10,16,18,20). These conditions can mean missed opportunities for isolating members of this genus.…”

Section: Discussionmentioning

confidence: 99%

“…These conditions can mean missed opportunities for isolating members of this genus. BS agar is the only medium which allows for the isolation of these atypical strains (1, 2,13,16,20). However, preparation, storage time, and temperature can affect the selective and a See footnote c of Table 5.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to subgroup 1, where lactose fermentation is seldom reported, 60% of Salmonella subgroup 3 strains (Arizona spp.) ferment this carbohydrate (7,8,16). Strains belonging to subgroup 3 can be found from a variety of animal sources (5)(6)(7)(8)10), some of which are common foods for human consumption.…”

mentioning

confidence: 99%

“…Bismuth sulfite (BS) agar has been the only recommended medium to date which allows for detection of biochemically atypical salmonellae, especially the lactose-fermenting strains (1, 2,13,16,20). However, the selectivity and differential properties of this medium can change dramatically as a result of preparation and storage conditions (3,4).…”

mentioning

confidence: 99%

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Novobiocin-brilliant green-glucose agar: new medium for isolation of salmonellae

Devenish¹,

Ciebin²,

Brodsky³

1986

Appl Environ Microbiol

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A new medium, called novobiocin-brilliant green-glucose (NBG) agar, was developed for the isolation of Salmonella spp. and evaluated against other conventionally used media including bismuth sulfite, xylose-lysine decarboxylase, brilliant green-sulfa, hektoen enteric, and salmonella-shigella agars. NBG had recovery rates comparable to the other enteric media tested with pure cultures as well as with naturally contaminated amphibian and reptile waters and fecal specimens. However, NBG, hektoen enteric, and salmonella-shigella agars failed to differentiate Salmonella typhi from a fecal specimen even after enrichment in selenite F. Although Citrobacterfreundii could grow and resembled salmonellae on NBG, at no time was the recovery of Salmonella spp. colonies jeopardized by the presence of C. freundii in either seeded or naturally contaminated samples. Confirmation rates of typical colonies from NBG agar also compared favorably to the other media tested; however, bismuth sulfite, although selective, was found to have varied differential characteristics for Salmonella spp. As a result, many more colonies had to be picked, which caused bismuth sulfite agar to have the lowest confirmation rate of the media tested. The distinct advantage that NBG agar offers over the conventional method tested, including bismuth sulfite, is the consistent differential reaction of all Salmonella subgroups including biochemically atypical strains. The medium is inexpensive, easy to prepare, and can be stored for at least 2 weeks at 4°C without loss of selective or differential properties.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Novobiocin-brilliant green-glucose agar: new medium for isolation of salmonellae

Devenish¹,

Ciebin²,

Brodsky³

1986

Appl Environ Microbiol

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Salmonella Infections

Shivaprasad

Barrow

2013

Diseases of Poultry

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Evaluation of a New Chromogenic Medium for the Isolation and Presumptive Identification of Salmonella Species from Stool Specimens

Eigner

Reissbrodt

Hammann

et al. 2001

Eur J Clin Microbiol Infect Dis

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The performance of BBL CHROMagar Salmonella (Becton Dickinson, France), a new selective chromogenic medium for the isolation and presumptive identification of Salmonella spp., was evaluated. On this medium, which is a modification of CHROMagar Salmonella (CHROMagar Microbiology, France) with enhanced selectivity, the colonies of Salmonella are stained in mauve (rose-violet), while those of other organisms appear in blue-green or are not stained by any of the chromogens of the medium. The medium was evaluated with a total of 176 strains of Salmonella and other organisms, consisting of 18 reference strains and 158 clinical isolates. All Salmonella strains except subspecies IIIa and IIIb strains and Salmonella Gallinarum yielded typical mauve colonies. During the evaluation with 107 known positive and 332 unknown stool specimens in a clinical laboratory, a total of 115 and 105 Salmonella isolates were obtained on BBL CHROMagar Salmonella and Hektoen enteric agar, respectively. From the known positive stool specimens, 92 true positive cultures were obtained on BBL CHROMagar Salmonella and 89 on Hektoen enteric agar, yielding sensitivities of 86 and 83%, respectively. From the unknown stool specimens, a total of 27 Salmonella isolates were obtained, with 23 isolated from BBL CHROMagar Salmonella and 16 from Hektoen enteric agar by direct plating (sensitivity 85 and 59%, specificity 99 and 97%, respectively). Seroagglutination tests could be performed directly from BBL CHROMagar Salmonella. Compared to conventional isolation media, the time needed for confirmatory biochemical and serological tests was shortened by about 1 day when BBL CHROMagar Salmonella was used. On the basis of these results, the medium can be recommended for the primary isolation and presumptive identification of Salmonella spp. from clinical stool specimens.

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Plating Medium for Differentiating Salmonella arizonae from Other Salmonellae

Abstract: A differential plating medium developed for isolation of Salmonella arizonae produces a uniform reaction for both lactose-negative and -positive S. arizonae and differentiates S. arizonae from other salmonellae.

Cited by 6 publications

References 4 publications

Novobiocin-brilliant green-glucose agar: new medium for isolation of salmonellae

Novobiocin-brilliant green-glucose agar: new medium for isolation of salmonellae

Salmonella Infections

Evaluation of a New Chromogenic Medium for the Isolation and Presumptive Identification of Salmonella Species from Stool Specimens

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