Plating Isolation of Various Catalase-Negative Microorganisms from Soil

Agromyces ramosus, which is a numerically prevalent bacterium in soil, was easily induced into the L-phase by growing it on agar media containing low levels of penicillin or glycine. The L-forms were stable after initial contact with the inducing agent and could not be reverted to the bacterial form by any of the procedures tried. These results are discussed in relation to a possible natural occurrence of L-forms of this bacterium in soil.

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L-Phase variants ofAgromyces ramosus

Horwitz

Casida

1975

Antonie van Leeuwenhoek

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Interaction of Agromyces ramosus with Other Bacteria in Soil

1983

Appl Environ Microbiol

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Agromyces ramosus occurs in very high numbers in most soils and, based on studies of laboratory isolates, does not require host cells for growth. Nevertheless, it attacked and destroyed most of the gram-positive and gram-negative bacterial species tested as possible host organisms. A. ramosus also attacked and destroyed Saccharomyces cerevisiae. The possibility of attack on fungi was unclear. Among the bacteria serving as hosts were the important soil species Azotobacter vinelandii, Rhizobium leguminosarum, Rhizobium meliloti , and Agrobacterium tumefaciens. Dead cells were not attacked. A. vinelandii cysts were attacked but left unharmed. To some extent, A. vinelandii seemed to survive this attack by encysting. Attack by A. ramosus occurred in natural soil and over a broad range of nutritional levels in laboratory media. The attack did not seem to be a means for obtaining an increased supply of commonly available nutrients. Instead, it seemed to be a means of obtaining something produced, perhaps in small amounts, by a variety of organisms, but not by all organisms. Several types of culture filtrates were tested for activity. The filtrates neither stimulated nor inhibited the growth of A. ramosus or the host organisms. The availability of catalase activity in host organisms did not seem to be involved. It is not known whether the attack by Agromyces ramosus in soil can be manipulated to cause a decrease in numbers of Agrobacterium tumefaciens or other pathogens without simultaneously depressing the numbers of beneficial organisms in this habitat.

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Plating Isolation of Various Catalase-Negative Microorganisms from Soil

Abstract: A procedure using nutritionally minimal media is presented for the plating isolation, but not enumeration, of various catalase-negative microorganisms from soil.

Cited by 2 publications

References 6 publications

L-Phase variants ofAgromyces ramosus

L-Phase variants ofAgromyces ramosus

Interaction of Agromyces ramosus with Other Bacteria in Soil

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