Platform with biomimetic electrochemical sensors for adiponectin and leptin detection in human serum

Mihailescu, Carmen-Marinela; Stan, Dana; Savin, Mihaela; Moldovan, Carmen; Dinulescu, Silviu; Radulescu, Clara Hortensia; Firtat, Bogdan; Muscalu, George; Brasoveanu, Costin; Ion, Marian; Dragomir, David; Stan, Diana; Ion, Alina Catrinel

doi:10.1016/j.talanta.2019.120643

Cited by 19 publications

(10 citation statements)

References 29 publications

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“…Ince et al presented a sensitive immunosensor system for adiponectin detection (25–2500 pg mL –1 ) . The detection range for adiponectin in our study is also wider than that of the biosensing system reported by Ince et al The proposed GP-based biosensor also showed a lower detection limit compared to the reported studies , given in Table . Moreover, the constructed GP-based biosensor could be prepared more easily compared to that reported by Lee et al As clearly seen in Table , the GP-based biosensor has advantages such as easy preparation, low cost, and ultrahigh sensitivity compared to the other studies.…”

Section: Resultscontrasting

confidence: 40%

“…It is an anti-inflammatory adipokine and plays a crucial role in glucose and lipid metabolism. All of these relationships have suggested that adiponectin is a possible biomarker of the metabolic syndrome. , Adiponectin, a member of the adipokine family, is involved in many important biological events . It was first recognized that adiponectin is a dysregulated adipose tissue peptide in obesity.…”

Section: Introductionmentioning

confidence: 99%

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Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Özcan

Sezgintürk

2021

ACS Biomater. Sci. Eng.

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This study presents a disposable, novel, and sensitive biosensing system to determine adiponectin, an obesity biomarker, in real human serum. The graphite paper (GP) working electrode is a new material for impedimetric biosensors. In the literature, there is no study in which this electrode is used in impedance-based biosensors for adiponectin detection. Sensitive and useful techniques, such as electrochemical impedance spectroscopy and cyclic voltammetry, were utilized for investigation of the modification of the GP electrode surface and optimization and characterization of the constructed biosensor. The single frequency impedance technique was used to study the interactions between antiadiponectin and adiponectin. The morphology of the electrode surface for each immobilization step was examined with scanning electron microscopy. All experimental parameters were optimized to fabricate a rapid and sensitive biosensing system. The designed biosensor presents excellent performance with a wide detection range (0.05–25 pg mL–1) and a low limit of detection (0.0033 pg mL–1) for adiponectin determination. Also, it has been demonstrated that the biosensor sensitively allows for the detection of adiponectin in human serum. The affinity of the designed immunosensor toward other proteins and components was examined in the presence of the target protein (adiponectin), leptin (100 pg mL–1), creatine kinase (50 pg mL–1), parathyroid hormone (50 pg mL–1), and d-glucose (0.5 M). The selectivity of the adiponectin biosensor resulted in high capacity to neglect the interference effect. The constructed biosensor showed good linearity, long-term storage life (10 weeks), high reusability (18 times regenerability), and high ability to detect adiponectin concentrations at picogram levels.

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Section: Resultscontrasting

confidence: 40%

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Özcan

Sezgintürk

2021

ACS Biomater. Sci. Eng.

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“…Molecularly imprinted electrochemical sensors are a type of MIP that synergistically combines the advantages of MIP (in particular their selectivity) with those of electrochemical sensors, such as high sensitivity, simple operation, portability, low cost, user friendliness, and fast analysis. , The molecular imprinted electrochemical sensors based on graphene, nanocomposites, carbon nanotubes, and carbon nitride nanotubes , have been intensely used for analysis in last years. In terms of research over the past few years, efforts have been focused on the development of molecularly imprinted electrochemical sensors based on the synthesis of polymers with a predetermined selectivity for a particular target substance …”

Section: Introductionmentioning

confidence: 99%

Impedimetric Sensors for Cyclocreatine Phosphate Determination in Plasma Based on Electropolymerized Poly(o-phenylenediamine) Molecularly Imprinted Polymers

Abo-Elmagd¹,

Mahmoud

Al‐Ghobashy

et al. 2021

ACS Omega

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Cyclocreatine and its water-soluble derivative, cyclocreatine phosphate (CCrP), are potent cardioprotective drugs. Based on recent animal studies, CCrP, FDA-awarded Orphan Drug Designation, has a promising role in increasing the success rate of patients undergoing heart transplantation surgery by preserving donor hearts during transportation and improving the recovery of transplanted hearts in recipient patients. In addition, CCrP is under investigation as a promising treatment for creatine transporter deficiency, an X-linked inborn error resulting in a poor quality of life for both the patients and the caregiver. A newly designed molecularly imprinted polymer (MIP) material was fabricated by the anodic electropolymerization of o-phenylenediamine on screen-printed carbon electrodes and was successfully applied as an impedimetric sensor for CCrP determination to dramatically reduce the analysis time during both the clinical trial phases and drug development process. To enhance the overall performance of the proposed sensor, studies were performed to optimize the electropolymerization conditions, incubation time, and pH of the background electrolyte. Scanning electron microscopy, electrochemical impedance spectroscopy, and cyclic voltammetry were used to characterize the behavior of the developed ultrathin MIP membrane. The CCrP-imprinted polymer has a high recognition affinity for the template molecule because of the formation of 3D complementary cavities within the polymer. The developed MIP impedimetric sensor had good linearity, repeatability, reproducibility, and stability within the linear concentration range of 1 × 10–9 to 1 × 10–7 mol/L, with a low limit of detection down to 2.47 × 10–10 mol/L. To verify the applicability of the proposed sensor, it was used to quantify CCrP in spiked plasma samples.

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“…Contrarily to detection approaches commonly proposed by Scheller and his collaborators, here, imprinted polyscopoletin is applied to impedimetric detection. Only very recently, a work proposing an impedimetric sensor based on imprinted polyscopoletin has appeared in literature [78]. Obtained impedimetric data are not rigorously treated and are not characterized by the expected semicircle feature, thus leaving unclear the way for estimating the resistance value to be used as analytical signal.…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polyscopoletin for the Electrochemical Detection of the Chronic Disease Marker Lysozyme

2020

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Herein we report the electropolymerization of a scopoletin based molecularly imprinted polymer (MIP) for the detection of lysozyme (Lyz), an enzymatic marker of several diseases in mammalian species. Two different approaches have been used for the imprinting of lysozyme based, respectively, on the use of a monomer-template mixture and on the covalent immobilization of the enzyme prior to polymer synthesis. In the latter case, a multi-step protocol has been exploited with preliminary functionalization of gold electrode with amino groups, via 4-aminothiophenol, followed by reaction with glutaraldehyde, to provide a suitable linker for lysozyme. Each step of surface electrode modification has been followed by cyclic voltammetry and electrochemical impedance spectroscopy, which has been also employed to test the electrochemical responses of the developed MIP. The sensors show good selectivity to Lyz and detect the enzyme at concentrations up to 292 mg/L (20 μM), but with different performances, depending on the used imprinting approach. An imprinting factor equal to 7.1 and 2.5 and a limit of detection of 0.9 mg/L (62 nM) and 2.1 mg/L (141 nM) have been estimated for MIPs prepared with and without enzyme immobilization, respectively. Competitive rebinding experiment results show that this sensing material is selective for Lyz determination. Tests were performed using synthetic saliva to evaluate the potential application of the sensors in real matrices for clinical purposes.

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Platform with biomimetic electrochemical sensors for adiponectin and leptin detection in human serum

Cited by 19 publications

References 29 publications

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Impedimetric Sensors for Cyclocreatine Phosphate Determination in Plasma Based on Electropolymerized Poly(o-phenylenediamine) Molecularly Imprinted Polymers

Molecularly Imprinted Polyscopoletin for the Electrochemical Detection of the Chronic Disease Marker Lysozyme

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