2010
DOI: 10.1016/j.vaccine.2010.07.084
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Platform technology to deliver prophylactic molecules orally: An example using the Class A select agent Yersinia pestis

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Cited by 16 publications
(22 citation statements)
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“…Analysis of localization and lipidation of the five vaccine constructs used in this study revealed that the OspA signal peptide tagged OspC K, OspB, BBK32, Salp15 and Salp25 for translocation across the cytoplasmic membrane, given that these proteins were primarily found in the envelope fraction (>60%) as opposed to the cytosolic fraction of the cell. Furthermore, OspC K, BBK32 and Salp15 constructs partitioned mostly into the detergent phase (Figure 3-1), the hydrophobic fraction of the E. coli cell envelope, consistent with protein lipidation and with our previous findings [110,114,115].…”
Section: Chapter 4 Discussion and Concluding Remarks Discussionsupporting
confidence: 89%
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“…Analysis of localization and lipidation of the five vaccine constructs used in this study revealed that the OspA signal peptide tagged OspC K, OspB, BBK32, Salp15 and Salp25 for translocation across the cytoplasmic membrane, given that these proteins were primarily found in the envelope fraction (>60%) as opposed to the cytosolic fraction of the cell. Furthermore, OspC K, BBK32 and Salp15 constructs partitioned mostly into the detergent phase (Figure 3-1), the hydrophobic fraction of the E. coli cell envelope, consistent with protein lipidation and with our previous findings [110,114,115].…”
Section: Chapter 4 Discussion and Concluding Remarks Discussionsupporting
confidence: 89%
“…Our vaccine technology consisted of recombinant live E. coli expressing the leader sequence of B. burgdorferi OspA lipoprotein fused to the N-terminus of each candidate immunogen (OspC K, OspB, BBK32, Salp15 or Salp25), aiming to target them across the cytoplasmic membrane to the cell envelope as previously shown [110,114,115]. Analysis of localization and lipidation of the five vaccine constructs used in this study revealed that the OspA signal peptide tagged OspC K, OspB, BBK32, Salp15 and Salp25 for translocation across the cytoplasmic membrane, given that these proteins were primarily found in the envelope fraction (>60%) as opposed to the cytosolic fraction of the cell.…”
Section: Chapter 4 Discussion and Concluding Remarks Discussionmentioning
confidence: 99%
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“…More recently, live bacterial vaccine vectors expressing Y. pestis virulence factors have been investigated for use as mucosal vaccines, which do not require injection and should produce higher levels of protection at mucosal surfaces than parenteral injection. Examples include the lactic acid commensal bacteria, Lactobacillus plantarum and an attenuated form of Salmonella enterica [1820]. However, these live vector vaccines have yet to be fully evaluated.…”
Section: Introductionmentioning
confidence: 99%