Platelets Proteomic Profiles of Acute Ischemic Stroke Patients

Abstract

Platelets play a crucial role in the pathogenesis of stroke and antiplatelet agents exist for its treatment and prevention. Through the use of LC-MS based protein expression profiling, platelets from stroke patients were analyzed and then correlated with the proteomic analyses results in the context of this disease. This study was based on patients who post ischemic stroke were admitted to hospital and had venous blood drawn within 24 hrs of the incidence. Label-free protein expression analyses of the platelets’ tryptic digest was performed in triplicate on a UPLC-ESI-qTOF-MS/MS system and ProteinLynx Global Server (v2.5, Waters) was used for tandem mass data extraction. The peptide sequences were searched against the reviewed homo sapiens database (www.uniprot.org) and the quantitation of protein variation was achieved through Progenesis LC-MS software (V4.0, Nonlinear Dynamics). These Label-free differential proteomics analysis of platelets ensured that 500 proteins were identified and 83 of these proteins were found to be statistically significant. The differentially expressed proteins are involved in various processes such as inflammatory response, cellular movement, immune cell trafficking, cell-to-cell signaling and interaction, hematological system development and function and nucleic acid metabolism. The expressions of myeloperoxidase, arachidonate 12-Lipoxygenase and histidine-rich glycoprotein are involved in cellular metabolic processes, crk-like protein and ras homolog gene family member A involved in cell signaling with vitronectin, thrombospondin 1, Integrin alpha 2b, and integrin beta 3 involved in cell adhesion. Apolipoprotein H, immunoglobulin heavy constant gamma 1 and immunoglobulin heavy constant gamma 3 are involved in structural, apolipoprotein A-I, and alpha-1-microglobulin/bikunin precursor is involved in transport, complement component 3 and clusterin is involved in immunity proteins as has been discussed. Our data provides an insight into the proteins that are involved in the platelets’ activation response during ischemic stroke. It could be argued that this study lays the foundation for future mechanistic studies.

Platelet Proteome Changes in Dogs with Chronic Heart Failure

“…Blood samples were collected from the cephalic veins into EDTA containing tubes for complete blood counting, and acid citrate dextrose (ACD) containing tubes for platelet isolation (12), as well as into anticoagulant-free tubes for routine serum biochemistry analysis.…”

“…A volume of 20 ml of venous blood was collected into the tubes with ACD containing trisodium citrate (22.0 g/L), citric acid (8.0 g/L) and dextrose (24.5 g/L) (BD Vacutainer), and platelets were then isolated according to the method modi ed from Cevik et al (12). Brie y, platelet isolation steps included:…”

“…Every injected sample is a separate biological replicate. Steps of platelet proteomic analysis were performed in a total of 20 different biological samples, as described in a previous paper (12). Before starting the analysis, the detector and calibration settings were made by the MassLynx program (V4.1-Waters) which is speci c to Xevo G2-XS Q TOF (Waters) device where the analysis were performed.…”

“…Modern platelet proteomic studies can reveal quantitative and posttransitional changes in proteins, protein-protein interactions and protein localisations (10). Thus, platelet proteomics studies allow the characterization and elaboration of the basic biological, molecular and cellular functions of diseased and healthy conditions (10)(11)(12).…”

Особенности Протеома При Эндотелиальной Дисфункции На Фоне Хронических Сердечно-Сосудистых Заболеваний

“…Известно, что CD44 участвует в межклеточных взаимодействиях, а также во взаимодействиях клеток с межклеточным матриксом. Белок реализует процессы пролиферации и дифференциации ЭК [3].…”