Platelets lipidomics study of blood stasis rat model by using liquid chromatography‐tandem mass spectrometry

Sun, Mingqian; Li, Ying; Hu, Guang; Yang, Huizhen; Zhang, Jianan; Miao, Lei; Zhang, Ying; Lin, Li Jen; Liu, Jianxun

doi:10.1002/jssc.202200611

Cited by 3 publications

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metabolomics is a technique to monitor the dynamic changes of a biological system to physiological or pathological stimuli [8]. Lipomics is a branch of metabolomics, which studies all lipids in organisms [9]. Metabolomics and lipidomics have been widely applied to the studies of different kidney diseases including renal fibrosis, chronic kidney disease, diabetic nephropathy, and acute renal failure [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Integrated gas chromatography‐mass spectrometry and ultra‐high‐performance liquid chromatography‐mass spectrometry renal metabolomics and lipidomics deciphered the metabolic regulation mechanism of Gushudan on kidney‐yang‐deficiency‐syndrome rats

Lü

Zhang

Xin

et al. 2023

J of Separation Science

View full text Add to dashboard Cite

Kidney‐yang‐deficiency‐syndrome is a neuroendocrine disease caused by the dysfunction of the adrenal‐pituitary‐target gland axis. Gushudan is a traditional Chinese medicine prescription with the functions of tonifying the kidney and strengthening bone, and its bone‐strengthening effect has been confirmed by previous anti‐osteoporosis research. However, its kidney‐tonifying mechanism has not been clear so far. In this study, renal metabolomics and lipidomics based on gas chromatography‐mass spectrometry and ultra‐high‐performance liquid chromatography‐high resolution mass spectrometry were integrated to find the metabolic disorders in kidney‐yang‐deficiency‐syndrome rats. Protein precipitation and liquid‐liquid extraction were used to extract metabolome and lipidome from the kidney. Gushudan regulated abnormal levels of amino acids, lipids, purines, and carbohydrates, such as L‐arginine, hypoxanine, stearic acid, and phosphatidylethanolamine (P‐18:1/20:4), which had effects on many metabolic pathways, such as glycerophospholipid metabolism, sphingolipid metabolism, glycine, serine and threonine metabolism and purine metabolism, and so forth. By integrating metabolomics and lipidomics, this study comprehensively revealed the abnormal metabolic activities of amino acids, lipids, and nucleotides in kidney‐yang‐deficiency‐syndrome, and the metabolic regulation mechanism of Gushudan in preventing kidney‐yang‐deficiency‐syndrome, as well as the improvement of Gushudan in maintaining renal cell structure, mitochondrial function, and energy supply, which also provided some new evidence and connotation for “kidney‐bone” axis.

show abstract

Section: Introductionmentioning

confidence: 99%

Integrated gas chromatography‐mass spectrometry and ultra‐high‐performance liquid chromatography‐mass spectrometry renal metabolomics and lipidomics deciphered the metabolic regulation mechanism of Gushudan on kidney‐yang‐deficiency‐syndrome rats

Lü

Zhang

Xin

et al. 2023

J of Separation Science

View full text Add to dashboard Cite

show abstract

Correlation Between Blood Cadmium Levels and Platelet Characteristics, As Well As Their Impact on Susceptibility to Coronary Heart Disease: Findings from NHANES 2005–2018 Data

Ci,

Zhai,

Wang

et al. 2024

Cardiovasc Toxicol

View full text Add to dashboard Cite

Machine learning insights into thrombo-ischemic risks and bleeding events through platelet lysophospholipids and acylcarnitine species

Harm,

Fu,

Frey

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Coronary artery disease (CAD) often leads to adverse events resulting in significant disease burdens. Underlying risk factors often remain inapparent prior to disease incidence and the cardiovascular (CV) risk is not exclusively explained by traditional risk factors. Platelets inherently promote atheroprogression and enhanced platelet functions and distinct platelet lipid species are associated with disease severity in patients with CAD. Lipidomics data were acquired using mass spectrometry and processed alongside clinical data applying machine learning to model estimates of an increased CV risk in a consecutive CAD cohort (n = 595). By training machine learning models on CV risk measurements, stratification of CAD patients resulted in a phenotyping of risk groups. We found that distinct platelet lipids are associated with an increased CV or bleeding risk and independently predict adverse events. Notably, the addition of platelet lipids to conventional risk factors resulted in an increased diagnostic accuracy of patients with adverse CV events. Thus, patients with aberrant platelet lipid signatures and platelet functions are at elevated risk to develop adverse CV events. Machine learning combining platelet lipidome data and common clinical parameters demonstrated an increased diagnostic value in patients with CAD and might improve early risk discrimination and classification for CV events.

show abstract

Platelets lipidomics study of blood stasis rat model by using liquid chromatography‐tandem mass spectrometry

Cited by 3 publications

References 29 publications

Integrated gas chromatography‐mass spectrometry and ultra‐high‐performance liquid chromatography‐mass spectrometry renal metabolomics and lipidomics deciphered the metabolic regulation mechanism of Gushudan on kidney‐yang‐deficiency‐syndrome rats

Integrated gas chromatography‐mass spectrometry and ultra‐high‐performance liquid chromatography‐mass spectrometry renal metabolomics and lipidomics deciphered the metabolic regulation mechanism of Gushudan on kidney‐yang‐deficiency‐syndrome rats

Correlation Between Blood Cadmium Levels and Platelet Characteristics, As Well As Their Impact on Susceptibility to Coronary Heart Disease: Findings from NHANES 2005–2018 Data

Machine learning insights into thrombo-ischemic risks and bleeding events through platelet lysophospholipids and acylcarnitine species

Contact Info

Product

Resources

About