Platelets are a possible regulator of human endometrial re‐epithelialization during menstruation

Abstract

These results indicate the possible involvement of platelets in the endometrial epithelial re-epithelialization process.

Platelet‐derived microparticles and soluble factors differentially regulate human endometrial epithelial cell movement

“…Although EM‐E6/E7/hTERT cells showed a widespread shape in the control culture, the intracellular actin stress fibers were aligned in a textile pattern (Figure A). After coculture with Plt‐CM, EM‐E6/E7/hTERT cells became polygonal in shape and intracellular cortical actin ring formation was significantly increased (Figure C,D), which is compatible with the previous findings showing an increase in cortical actin ring formation by platelets . On the other hand, Plt‐CM‐mp − did not promote cortical actin ring formation (Figure B).…”

“…In this study, to investigate the physiological roles of platelets in the re‐epithelialization process, we used EM‐E6/E7/hTERT cells . EM‐E6/E7/hTERT is an immortalized human endometrial epithelial cell line that maintains several characteristics of normal glandular epithelial cells, such as the ability to form a glandular structure on three‐dimensional culture .…”

“…Human platelets were isolated from healthy volunteers in the menstrual phase (females, 22‐38 years old, n=30), as described previously . Isolated platelets were suspended in PBS plus (PBS containing 0.9 mM Ca 2+ and 0.33 mM Mg 2+ ) at a concentration of 4×10 8 platelets/mL and were immediately used in subsequent experiments.…”

“…Recently, we demonstrated that extravascular platelets were deposited in the subluminal stroma during the menstrual phase. Although platelet‐derived key factors remain unknown, we also showed that the conditioned media (CM) of platelets enhanced cell‐to‐cell contact and cell‐to‐extracellular matrix attachment of a human endometrial epithelial cell‐derived immortalized cell line, EM‐E6/E7/hTERT cells, proposing the roles of platelets to promote re‐epithelialization by endometrial epithelial cells …”

Crystalline structure of pulverized dental calculus induces cell death in oral epithelial cells

“…The permeability assay was performed following the previously described procedure with slight modifications . HSC‐2 (2 × 10 5 ) cells were incubated in the upper insert of a transwell membrane (Corning, NY, USA).…”