Platelets and Neutrophils from Healthy Term Neonates Exhibit Increased Levels of Immunoglobulins

Silliman, Christopher C.; Cusack, Nancy; Swanson, Nancy J; Ghaffarifar, Simin; Ambruso, Daniel R.

doi:10.1203/00006450-199512000-00027

Cited by 7 publications

(2 citation statements)

References 28 publications

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“…The other 10 ml whole blood underwent identical separation, and the cells were used to obtain cell counts using a Coulter blood Plus IV cell counter (Beckman Coulter, Houston, TX). Following incubation, the blood was spun at 3000 g for 5 min to isolate platelet-rich plasma, which was separated from the red cells and the leukocytes, and the platelets were isolated from the plasma by centrifugation [26,27]. The remaining cells underwent dextran sedimentation, which isolated the majority of the red blood cells from the leukocytes, and the supernatant was separated into mononuclear and polymorphonuclear leukocytes by ficollhypaque gradient centrifugation.…”

Section: Pmn Isolation and Oxidase Primingmentioning

confidence: 99%

Lysophosphatidylcholines prime the NADPH oxidase and stimulate multiple neutrophil functions through changes in cytosolic calcium

Silliman

Elzi

Ambruso

et al. 2003

Journal of Leukocyte Biology

129

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A mixture of lysophosphatidylcholines (lyso-PCs) are generated during blood storage and are etiologic in models of acute lung injury. We hypothesize that lyso-PCs stimulate polymorphonuclear neutrophils (PMNs) through Ca(2)(+)-dependent signaling. The lyso-PC mix (0.45-14.5 micro M) and the individual lyso-PCs primed formyl-Met-Leu-Phe (fMLP) activation of the oxidase (1.8- to 15.7-fold and 1.7- to 14.8-fold; P<0.05). Labeled lyso-PCs demonstrated a membrane association with PMNs and caused rapid increases in cytosolic Ca(2)(+). Receptor desensitization studies implicated a common receptor or a family of receptors for the observed lyso-PC-mediated changes in PMN priming, and cytosolic Ca(2)(+) functions were pertussis toxin-sensitive. Lyso-PCs caused rapid serine phosphorylation of a 68-kD protein but did not activate mitogen-activated protein kinases or cause changes in tyrosine phosphorylation. With respect to alterations in PMN function, lyso-PCs caused PMN adherence, increased expression of CD11b and the fMLP receptor, reduced chemotaxis, provoked changes in morphology, elicited degranulation, and augmented fMLP-induced azurophilic degranulation (P<0.05). Cytosolic Ca(2)(+) chelation inhibited lyso-PC-mediated priming of the oxidase, CD11b surface expression, changes in PMN morphology, and serine phosphorylation of the 68-kD protein. In conclusion, lyso-PCs affect multiple PMN functions in a Ca(2)(+)-dependent manner that involves the activation of a pertussis toxin-sensitive G-protein.

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Section: Pmn Isolation and Oxidase Primingmentioning

confidence: 99%

Lysophosphatidylcholines prime the NADPH oxidase and stimulate multiple neutrophil functions through changes in cytosolic calcium

Silliman

Elzi

Ambruso

et al. 2003

Journal of Leukocyte Biology

129

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“…Therefore, the exact signi cance of PAIgG in neonates is still uncertain. Silliman et al found that platelets from healthy neonates have higher levels of IgG than do adult controls (39). In addition, Castle et al measured PAIgG on 177 thrombocytopenic neonates over a 1 y period (7) and observed elevated PAIgG in 52% of the affected infants, but also in 15% of the control, non-thrombocytopenic neonates.…”

Section: Platelet Antibody Testsmentioning

confidence: 99%

Mechanisms underlying thrombocytopenia in the neonatal intensive care unit

Sola

Rimsza

2002

Acta Paediatrica

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Sola MC, Rimsza LM. Mechanisms underlying thrombocytopenia in the neonatal intensive care unit. Acta Paediatr 2002; Suppl 438: 66-73. Stockholm. ISSN 0803-5326Thrombocytopenia is one of the most common hematological problems among neonates in the neonatal intensive care unit (NICU), but in the majority of cases the kinetic mechanism responsible is unclear. This review focuses on both traditional and innovative methods used to evaluate the mechanisms responsible for thrombocytopenia in neonates, and analyzes the data generated from those methods. Conclusion:Results of studies using new methods for evaluating thrombocytopenia, coupled with recent descriptions of marrow megakaryocyte mass, suggest that decreased platelet production complicates most cases of thrombocytopenia among neonates in the NICU.

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Evaluation and treatment of severe and prolonged thrombocytopenia in neonates

Sola¹

2004

Clinics in Perinatology

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Platelets and Neutrophils from Healthy Term Neonates Exhibit Increased Levels of Immunoglobulins

Cited by 7 publications

References 28 publications

Lysophosphatidylcholines prime the NADPH oxidase and stimulate multiple neutrophil functions through changes in cytosolic calcium

Lysophosphatidylcholines prime the NADPH oxidase and stimulate multiple neutrophil functions through changes in cytosolic calcium

Mechanisms underlying thrombocytopenia in the neonatal intensive care unit

Evaluation and treatment of severe and prolonged thrombocytopenia in neonates

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