Platelet transfusion in patients with liver cirrhosis cross-talk with neutrophil: Prospective study

Hassouna, Mona Mahmoud; Mostafa, Mohammed Sayed; Omar, Hazem; Mohammed, Esraa Azab; Abdallah, Heba

doi:10.1016/j.htct.2022.08.009

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…Although platelet surface activation markers at baseline were low, we detected increased numbers of PNCs in patients with liver cirrhosis compared to healthy subjects ( Figure 3 C). This observation is supported by former studies [ 36 , 37 ].…”

Section: Discussionsupporting

confidence: 90%

Decreased Platelet Aggregation in Patients with Decompensated Liver Cirrhosis and TIPS Implantation

et al. 2023

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Transjugular intrahepatic portosystemic shunt (TIPS) implantation is an effective treatment of portal hypertension in patients with decompensated liver cirrhosis. However, some patients develop TIPS thrombosis with recurrence of portal hypertension. The role of platelets in TIPS thrombosis and the necessity of antiplatelet therapy is unclear. Therefore, we aimed to study platelet function in patients with liver cirrhosis prior to and after TIPS implantation. Platelet aggregation was tested in peripheral and portal-vein blood patient samples on the day (D) of TIPS implantation (D0), D4 and D30 following the procedure (platelet count above 100 × 103/µL, aspirin starting on D5) using whole-blood impedance aggregometry (WBIA) and light transmission aggregometry (LTA). In addition, surface platelet activation markers (P-selectin, activated GPIIb/IIIa) and platelet–neutrophil complexes (PNCs) were assessed by flow cytometry. Thrombin receptor activating peptide 6 (TRAP-6), adenosine diphosphate (ADP) and arachidonic acid (AA) were used as agonists. Healthy subjects were included as controls. Agonist-induced platelet aggregation was reduced (WBIA: TRAP-6 p < 0.01, ADP p < 0.01, AA p < 0.001; LTA: TRAP-6 p = 0.13, ADP p = 0.05, AA p < 0.01) in patients (D0, n = 13) compared with healthy subjects (n = 9). While surface activation markers at baseline were negligibly low, the percentage of PNCs was higher in patients than in controls (p < 0.05). ADP-induced P-selectin expression was increased (p < 0.001), whereas TRAP-6-induced GPIIb/IIIa activation was impaired (p < 0.001) in patients versus controls. PNC formation in response to agonists was not different between groups. Results did not differ between peripheral and portal-vein blood of patients (D0, n = 11) and did not change over time (D0, D4, D30) following TIPS implantation (n = 9). In summary, patients with decompensated liver cirrhosis display in vitro platelet aggregation defects in response to various agonists. Defective aggregation persists upon TIPS implantation. Therefore, we conclude that antiplatelet treatment to prevent TIPS thrombosis is questionable.

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Section: Discussionsupporting

confidence: 90%

Decreased Platelet Aggregation in Patients with Decompensated Liver Cirrhosis and TIPS Implantation

et al. 2023

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“…The enhancement of the number of neutrophils is usually due to the presence of bacteria or infection as well as the existence of autocrine, paracrine, and immune system modulating functions involved in liver diseases, including viral hepatitis, nonalcoholic steatohepatitis, alcoholic liver disease, liver fibrosis, cirrhosis, liver failure, and liver cancer (Liu et al, 2017;Tang et al, 2021). Neutrophils are the largest number of circulating leukocytes which participate in various processes of immune reactions (Rosales, 2018) and inflammation (Hassouna et al, 2022). For example, the worsening of liver occurs in the development of alcoholic liver disease (ALD) lead to produce the high number of neutrophils and migrate from the blood circulation to the tissues injury that regulated by chemokines, cytokines, and adhesion molecules (Yang et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective activity of ethanolic extract of Plectranthus amboinicus (lour.) spreng leaf in DMBA induced rats

Silitonga

Sinaga

Nugrahalia

et al. 2023

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“…platelet transfusions in patients with thrombocytopenia result in further increases in CD11b expression on neutrophils. 87 Despite this, one of the important questions is whether the neutrophil dysfunction is primarily a cellular problem or if it is caused by extracellular factors. There are multiple studies that attempt to support one or other of these concepts.…”

Section: Causes Of Neutrophil Dysfunction In Cirrhosismentioning

confidence: 99%

Circulating neutrophil anti-pathogen dysfunction in cirrhosis

Balazs,

Stadlbauer

2023

JHEP Reports

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Platelet transfusion in patients with liver cirrhosis cross-talk with neutrophil: Prospective study

Cited by 4 publications

References 13 publications

Decreased Platelet Aggregation in Patients with Decompensated Liver Cirrhosis and TIPS Implantation

Decreased Platelet Aggregation in Patients with Decompensated Liver Cirrhosis and TIPS Implantation

Hepatoprotective activity of ethanolic extract of Plectranthus amboinicus (lour.) spreng leaf in DMBA induced rats

Circulating neutrophil anti-pathogen dysfunction in cirrhosis

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