Platelet Storage Lesion in Second‐Generation Containers: Correlation with Platelet ATP Levels

Holme, Stein; Heaton, W. A. L.; Courtright, M.

doi:10.1111/j.1423-0410.1987.tb05069.x

Cited by 115 publications

(82 citation statements)

References 33 publications

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“…This close correlation between the aspects of the storage lesion and loss of metabolic activity was confirmed in the recently published results of Holme (34) showing the correlation between metabolic activity, ATP concentration and storage lesion.…”

Section: Csupporting

confidence: 72%

Storage of Human Red Blood Cells and Platelets

Solberg

1988

Upsala Journal of Medical Sciences

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Y 1. Storage renders erythrocytes more responsive to thermally induced morphological changes, especially the shedding of microvesicles. 4-8 week old cells can be morphologically "rejuvenated" by heating.2. If pH increases during storage of platelets an extensive loss of small particles occurs. The platelet disintegration is associated with a loss in the metabolic activity, discharge of LDH, increased suseptibility to phospholipid hydrolysis by phospholipase C and is found to be initiated during the actual preparation of platelet concentrates.3. Activation of platelets during preparation can be decreased by shortening the first centrifugation time or by using adenine in the anticoagulant. 4.A 4 hour prestorage of the whole blood unit prior to centrifugation strongly decreases the activation of platelets upon stimuli and results in platelet concentates much more stable to storage.

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Section: Csupporting

confidence: 72%

Storage of Human Red Blood Cells and Platelets

Solberg

1988

Upsala Journal of Medical Sciences

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“…ACD in contrast to CPD does not contain phosphate and is routinely used for the preparation of apheresis platelets. Effects similar to those observed in the absence of glucose during storage of platelets [1,21,22] have been observed in PCs prepared by apheresis [16]. These effects may be associated with the use of ACD solution in combination with the nonphosphate-additive solution PAS-2.…”

Section: Effects Of Phosphatementioning

confidence: 53%

“…The 'endogenous fuel' acting as a major substrate can be fatty acids [20]. On the other hand, depletion of glucose and, in the next step, significantly reduced levels of adenine nucleotides have been associated with loss of platelet viability [1,21,22]. In a study by Klinger et al [23] focusing on morphological changes, PCs prepared by apheresis were stored in either plasma or PAS-2 with a very limited plasma carryover (10%).…”

Section: Effects Of Glucosementioning

confidence: 99%

Storage of Platelets for Transfusion in Additive Solutions: Effects of Different Factors and Compounds

Gulliksson¹

2000

Transfus Med Hemother

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Platelet-additive solution (PAS) can be used as a substitute for plasma for the storage of platelet concentrates (PCs) in order to improve storage conditions, to recover plasma for other purposes, to avoid transfusion of large volumes of plasma to patients, and to make possible photochemical treatment for viral inactivation of PCs. For this reason, PAS should have a great potential for wide use in transfusion medicine. In the studies cited in this review, PCs were prepared from units of whole blood either by the platelet-rich plasma method or the buffy coat method. In some studies, PCs prepared by apheresis were used. The composition of the PASs used varied. The effects on platelet metabolism associated with different factors and compounds in PAS are only partly known. Available studies suggest that: 1) Presence of glucose in the platelet storage medium during the entire storage period is necessary for platelet metabolism. 2) Acetate is used as an additional substrate for platelet metabolism. Acetate also reduces the production of lactate by platelets, and by the formation of bicarbonate it maintains stable pH levels during storage. 3) The fall in pH can be rapid in PAS-containing media due to the very limited buffering capacity of PAS compared with that of plasma. 4) Platelets stored in PAS at a citrate concentration of 8 mmol/l produce only half the quantity of lactate as that of platelets at 14–26 mmol/l of citrate. 5) Free fatty acids from plasma can be used as substrate for platelet metabolism and are supposed to be made available by the hydrolysis of plasma triglycerides. 6) For PCs prepared by apheresis with ACD anticoagulant, presence of phosphate in PAS seems to be a critical factor to avoid low adenine nucleotide levels during storage.

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“…As PAS contains 27 mM of acetate, this suggests maintenance of platelet viability for $13 days of storage as our studies demonstrated. Some studies have suggested that enough plasma must be present during platelet storage to maintain glucose levels, 35,36 whereas others have indicated that residual glucose is not required. 37,38 Our studies have indicated that, depending on the storage conditions (mainly the storage bag), acetate can substitute for glucose to maintain platelet viability.…”

Section: Discussionmentioning

confidence: 99%

Exploratory studies of extended storage of apheresis platelets in a platelet additive solution (PAS)

Slichter

Corson

Jones

et al. 2014

Blood

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• Extended apheresis platelet storage is dependent on the collection method, storage in a storage solution, and storage bag composition.• The lifespan of the platelet is not intrinsic to the cell, and platelet viability is better maintained in vitro than in vivo.To evaluate the poststorage viability of apheresis platelets stored for up to 18 days in 80% platelet additive solution (PAS)/20% plasma, 117 healthy subjects donated platelets using the Haemonetics MCS1, COBE Spectra (Spectra), or Trima Accel (Trima) systems. Control platelets from the same subjects were compared with their stored test PAS platelets by radiolabeling their stored and control platelets with either 51 chromium or 111 indium. Trima platelets met Food and Drug Administration poststorage platelet viability criteria for only 7 days vs almost 13 days for Haemonetics platelets; ie, platelet recoveries after these storage times averaged 44 6 3% vs 49 6 3% and survivals were 5.4 6 0.3 vs 4.6 6 0.3 days, respectively. The differences in storage duration are likely related to both the collection system and the storage bag. The Spectra and Trima platelets were hyperconcentrated during collection, and PAS was added, whereas the Haemonetics platelets were elutriated with PAS, which may have resulted in less collection injury. When Spectra and Trima platelets were stored in Haemonetics' bags, poststorage viability was significantly improved. Platelet viability is better maintained in vitro than in vivo, allowing substantial increases in platelet storage times. However, implementation will require resolution of potential bacterial overgrowth during storage. (Blood. 2014;123(2):271-280)

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Platelet Storage Lesion in Second‐Generation Containers: Correlation with Platelet ATP Levels

Cited by 115 publications

References 33 publications

Storage of Human Red Blood Cells and Platelets

Storage of Human Red Blood Cells and Platelets

Storage of Platelets for Transfusion in Additive Solutions: Effects of Different Factors and Compounds

Exploratory studies of extended storage of apheresis platelets in a platelet additive solution (PAS)

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