Platelet storage for 7 days in second‐generation blood bags

Abstract

Plastic storage bags designed to optimize O2 and CO2 transfer to preserve platelets for 7 days prior to transfusion were studied in vivo and in vitro. Platelets stored 7 days in second-generation CLX bags were compared to platelets stored 3 days in standard (CL-3861) 3-day storage bags and platelets transfused within 24 hours of collection. The CLX bags maintained concentrate pH at a mean of 6.85 +/- 0.03 (SEM) after 7 days, while in standard bags after 3 days of storage, the mean pH was 6.46 +/- 0.03. A smaller proportion of platelets stored 7 days in CLX bags were discarded because of a pH less than 6.0 compared to those stored 3 days in CL-3861 bags (10 vs 21%). Poststorage pH showed strong correlation with concentrate platelet count and weak correlation with concentrate white cell count in both bag types. There was no significant difference in the mean corrected platelet count increments between platelets stored 7 days in second generation CLX bags and those stored 3 days in CL-3861 bags (10,000 and 12,200 at 1 hour, and 7000 and 7500 at 24 hours, respectively) following transfusion to 16 thrombocytopenic recipients. However, transfusion of fresh platelets achieved mean corrected increments at both 1 and 24 hours posttransfusion that were higher than seen with either group of stored platelets (20,100 at 1 hour and 10,800 at 24 hours). Platelets can be stored 7 days in second-generation CLX blood bags with results comparable to those of platelets stored 3 days in standard bags.

Prevention of Cytokine Accumulation in Platelets Obtained with the COBE Spectra Apheresis System

“…While we do not believe there is a meaningful effect from this variable, we cannot conclusively demonstrate that from the data presented here. However, previous studies have indicated that both the Miles CLX bags and Cobe Spectra’s ELP PVC bags are favorable for the extended storage of platelets [40, 41, 42, 43, 44]. …”

Factors affecting posttransfusion platelet increments, platelet refractoriness, and platelet transfusion intervals in thrombocytopenic patients

“…Longer platelet storage times have been reported to decrease posttransfusion platelet viability. [20][21][22][23] However, some investigators have suggested that storage adversely affects only platelet concentrates rather than apheresis platelets. 24 In our study, an equal benefit of platelets stored for 48 hours or less was seen for all the types of platelets transfused.…”

Platelet Transfusion Therapy

“…This combination of storage container, agitation, preservative solution, temperature, and the use of approximately 50 mL of plasma permits satisfactory preservation of platelets for up to 7 days 23,24 However, several instances of bacterial contamination of PCs stored for this period have been reported 25,26 and the storage time from collection to transfusion is is now limited to 5 days. 27 Because several units of PC are pooled to obtain a dose for one transfusion, one reason to use single-donor apheresis platelets is to minimize the number of donors to which the patient is exposed and, theoretically, to minimize the likelihood of disease transmission.…”