Platelet-rich plasma preparation using three devices: Implications for platelet activation and platelet growth factor release

Everts, Peter A.; Mahoney, Chris; Hoffmann, J. J. M. L.; Schönberger, Jacques P.A.M.; Box, Henk A. M.; Zundert, André van; Knape, J. T. A.

doi:10.1080/08977190600821327

Cited by 168 publications

(128 citation statements)

References 25 publications

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“…In particular, these include neutrophilic granulocytes and monocytes, both known for host defense mechanism actions against bacteria through the action of myeloperoxidase that creates hypochloric acid. The number of platelets in PLG is three to seven times higher than normally found, whereas the white blood cell count is two to four times greater than normal [9][10][11].…”

mentioning

confidence: 99%

The use of autologous platelet–leukocyte gels to enhance the healing process in surgery, a review

et al. 2007

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Background: The therapeutic use of autologously prepared, platelet-leukocyte-enriched gel (PLG) is a relatively new technology for the stimulation and acceleration of soft tissue and bone healing. The effectiveness of this procedure lies in the delivery of a wide range of platelet growth factors mimicking the physiologic wound healing and reparative tissue processes. Despite an increase in PLG applications, the structures and kinetics of this autogenously derived biologic material have not been observed. Methods: A review of the most recent literature was performed to evaluate the use of PLG in various surgical disciplines. Results: The review showed that the application of PLG has been extended to various surgical disciplines including orthopedics, cardiac surgery, plastic and maxillofacial surgery, and recently also endoscopic surgery. Conclusion: This review demonstrates the usefulness of PLG in a wide range of clinical applications for improvement of healing after surgical procedures.Key words: Growth factors -Leukocytes -Platelet gel -Wound healing Soft tissue wound healing and bone growth involve physiologic cascades in which cellular and hormonal factors play pivotal roles [1,2]. Some of these cascade components can be isolated from autologously drawn whole blood. Point-of-care devices can intraoperatively fractionate the autologous blood into platelet-poor plasma, platelet-leukocyte-rich plasma (P-LRP), and red blood cells [3,4]. The P-LRP fraction, a mixture of concentrated platelets and leukocytes, can be activated by (autologous) thrombin to create a viscous solution known as platelet-leukocyte gel (PLG). This platelet coagulum can be exogenously applied to soft wound tissues, bone, or synthetic bone as a spray or as a solid, clotted, gelatinous mass. The rationale for applying platelet gel is based on the delivery of platelet growth factors to tissues and on the fact that platelet a-granules, found inside the platelets, contain a variety of growth factors [5]. Platelet gel growth factors are peptides that promote cell proliferation, differentiation, chemotaxis, and the migration of various cells involved in both wound healing and bone growth [6,7].Recently, numerous P-LRP devices have become available for therapeutic use to stimulate and accelerate soft tissue and bone healing and to control postoperative wound bleeding. The rationale for applying PLG lies in the mimicking and accelerating of physiologic wound healing and reparative tissue processes.This article provides information on the results of electron microscopic imaging used to evaluate the content of PLG. Furthermore, because the use of PLG is a relatively new per-and/or perioperative biotechnological application, new and additional indications for the use of PLG are defined. New PLG applications are reviewed for different applications including cardiac surgery, general surgery, orthopedics and traumatology, cosmetic surgery, maxillofacial surgery, sports medicine, and endoscopic surgical procedures. Defining platelet-leukocyte-rich...

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The use of autologous platelet–leukocyte gels to enhance the healing process in surgery, a review

et al. 2007

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“…A sua utilização clínica em ortopedia vem crescendo, a despeito da ausência de estudos prospectivos e randomizados que avaliem os resultados de sua aplicação (30,(34)(35)(36) . Em um estudo que se propõe a avaliar a eficácia do PRP isolado ou em associação com outros materiais, é importante lembrar que diversos são os métodos utilizados na obtenção do PRP (1,5,30) , e que a capacidade em obter altas concentrações de plaquetas é variável entre os métodos disponíveis. A maioria dos sistemas utilizados na prática clínica é baseada na centrifugação; as centrífugas utilizadas para esse fim foram desenvolvidas para diagnóstico e não para a obtenção de PRP, que é gerado frequentemente com nível de plaquetas inferior ao ideal.…”

Section: Discussionunclassified

“…Para que o PRP tenha maior eficácia, a concentração ideal de plaquetas deve ser em torno de 1.000.000/µl (1,2,4) . Existem diversos métodos de obtenção de PRP (5) , cada um com sua peculiaridade em relação à capacidade de concentração das plaquetas, assim como no processo de liberação dos fatores de crescimento pelas plaquetas.…”

Section: Introductionunclassified

O uso do plasma rico em plaquetas associado ao aspirado de medular óssea na osteotomia tibial tipo puddu

D’Elia¹,

Rezende²,

Bitar³

et al. 2009

Rev. bras. ortop.

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rESUMOObjetivo: O presente estudo procurou avaliar a aplicação do plasma rico em plaquetas associado ao aspirado de medular ós-sea como substituto ósseo ao enxerto autólogo do ilíaco nas osteotomias tibiais proximais de cunha de adição medial (OTCAM). Métodos: Foram estudados 25 pacientes submetidos a OTCAM divididos de forma randomizada em dois grupos, grupo ilíaco, 14 pacientes submetidos a OTCAM nos quais se utilizou o enxerto autólogo do ilíaco para preencher o sitio da osteotomia, grupo PRP, 11 pacientes submetidos a OTCAM nos quais se utilizou um composto formado por plasma rico em plaquetas associado ao aspirado de medular ósseo para preencher o sitio da osteotomia. Foram avaliados o sangramento (variação dos níveis de hemoglobina e hematócrito) e a dor (escala visual analógica -EVA), comparando os grupos em relação a essas variáveis. Resultados: Não foram observadas diferenças entre os grupos no que se refere à variação dos níveis de hemoglobina (p = 0,820) e hematócrito (p = 0,323). Os grupos não foram diferentes em relação à intensidade da dor segundo a EVA (p = 0,538). Conclusão: O uso do PRP associado ao aspirado de medular óssea nas OTCAM não demonstrou vantagem sobre a utilização do enxerto autólogo do ilíaco no que se refere a dor e sangramento.

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“…These include three isomers of platelet derived growth factor -PDGF (αα, ββ, αβ), vascular endothelial growth factor -VEGF, transforming growth factor β -TGF β1, epithelial growth factor -EGF, insulin like growth factor -IGF 1, angiopoietin 2 (Ang 2), interleukin 1β (IL 1β) and many others. Platelet rich plasma is also a source of pro teins involved in cell adhesion, such as fibrin, fibronec tin, vitronectin, osteocalcin or osteonectin (Everts et al, 2006). The growth factors are released as a result of degranulation of thrombocyte granules soon after the coagulation cascade is initiated and bind the membrane receptors of adjacent cells, thus activating the intracel lular signalling pathway (Anitua et al, 2004;Eppley et al, 2004).…”

Section: Candida Parapsilosis 1mentioning

confidence: 99%

The Effect of Local Platelet Rich Plasma Therapy on the Composition of Bacterial Flora in Chronic Venous Leg Ulcer

Woźniak

Tarnas²,

Miłek

et al. 2016

Polish Journal of Microbiology

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Microbial colonisation of chronic venous ulcers and synergism between bacterial species slow down the healing process. The study aimed at performing qualitative analysis of microbial flora in venous leg ulcers treated with platelet rich plasma (PRP). Twenty two women and twelve men aged 47-90 years were treated with PRP at our department between 2012 and 2015. Ulcer cultures collected before and after PRP therapy yielded 83 and 110 microbial isolates, respectively, of Gram positive, Gram negative bacteria and candida. Pseudomonas aueruginosa and Staphylococcus aureus were the most common pre- and post-treatment isolates. PRP therapy and increased the variety of microbial flora.

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Platelet-rich plasma preparation using three devices: Implications for platelet activation and platelet growth factor release

Cited by 168 publications

References 25 publications

The use of autologous platelet–leukocyte gels to enhance the healing process in surgery, a review

The use of autologous platelet–leukocyte gels to enhance the healing process in surgery, a review

O uso do plasma rico em plaquetas associado ao aspirado de medular óssea na osteotomia tibial tipo puddu

The Effect of Local Platelet Rich Plasma Therapy on the Composition of Bacterial Flora in Chronic Venous Leg Ulcer

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