Platelet-rich plasma loaded nerve guidance conduit as implantable biocompatible materials for recurrent laryngeal nerve regeneration

Kim, Ji Won; Kim, Jeong Mi; Choi, Mi Eun; Jeon, Eun Jeong; Park, Jin-Mi; Kim, Young-Mo; Choi, Seung-Ho; Eom, Taesik; Shim, Bong Sup; Choi, Jeong Seok

doi:10.1038/s41536-022-00239-2

Cited by 12 publications

(8 citation statements)

References 61 publications

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“…The extracellular milieu of the surviving neurons was improved by 59 , 61 , demonstrating that the brain trophic factor BDNF-modified human BMSCs exhibited additive neuroprotective effects and that accelerated axonal sprouting NGF expression was higher in PRP-treated cells than in untreated controls. NGF expression was higher in the treated cells than in the untreated controls 62 , 63 which coincides with our results.…”

Section: Discussionsupporting

confidence: 92%

Combined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAP

Farid,

Yasin,

Al-Mokaddem

et al. 2024

Sci Rep

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A single injection of platelet-rich plasma (PRP) or stromal vascular fraction (SVF) in treating neurological ailments suggests promise; however, there is limited evidence of the efficacy of combination therapy. This trial aimed to determine whether combining SVF and PRP could provide further therapeutic effects in treating multiple sclerosis (MS). Fifteen Persian cats were separated into three groups (n = 5): group I (control negative), and group II (control positive); EB was injected intrathecally into the spinal cord and then treated 14 days later with intrathecal phosphate buffered saline injection, and group III (SVF + PRP), cats were injected intrathecally with EB through the spinal cord, followed by a combination of SVF and PRP 14 days after induction. Therapeutic effects were evaluated using the Basso–Beattie–Bresnahan scale throughout the treatment timeline and at the end. Together with morphological, MRI scan, immunohistochemical, transmission electron microscopy, and gene expression investigations. The results demonstrated that combining SVF and PRP successfully reduced lesion intensity on gross inspection and MRI. In addition to increased immunoreactivity to Olig2 and MBP and decreased immunoreactivity to Bax and GFAP, there was a significant improvement in BBB scores and an increase in neurotrophic factor (BDNF, NGF, and SDF) expression when compared to the positive control group. Finally, intrathecal SVF + PRP is the most promising and safe therapy for multiple sclerosis, resulting in clinical advantages such as functional recovery, MRI enhancement, and axonal remyelination.

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Section: Discussionsupporting

confidence: 92%

Combined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAP

Farid,

Yasin,

Al-Mokaddem

et al. 2024

Sci Rep

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“…Additionally, a variety of substances including collagen fibers, stem cells, and neurotrophic factors like nerve growth factor have been found to enhance regeneration of injured peripheral nerves. 41 Although stem cells implantation in NGCs have demonstrated useful effects on THE regeneration of injured peripheral nerves, but it has been found that the key critical mechanism for stem cells in peripheral nerve repair refers to the paracrine effect of stem cells rather than the differentiation potency of stem cells. 24 Additionally, many studies have demonstrated that the paracrine role of stem cells is related to their released exosomes as cell products.…”

Section: Discussionmentioning

confidence: 99%

“…So, this study was proved that the double layered SF/PLLA nerve guide conduit had acceptable biocompatibility and mechanical properties and can support cell attachment along with proliferation. Additionally, a variety of substances including collagen fibers, stem cells, and neurotrophic factors like nerve growth factor have been found to enhance regeneration of injured peripheral nerves . Although stem cells implantation in NGCs have demonstrated useful effects on THE regeneration of injured peripheral nerves, but it has been found that the key critical mechanism for stem cells in peripheral nerve repair refers to the paracrine effect of stem cells rather than the differentiation potency of stem cells .…”

Section: Discussionmentioning

confidence: 99%

Tissue-Engineered Core–Shell Silk-Fibroin/Poly-l-Lactic Acid Nerve Guidance Conduit Containing Encapsulated Exosomes of Human Endometrial Stem Cells Promotes Peripheral Nerve Regeneration

Namini

Ebrahimi‐Barough

et al. 2023

ACS Biomater. Sci. Eng.

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Nerve guide conduits (NGCs) have been shown to be less efficient than nerve autografts in peripheral nerve regeneration. To address this issue, we developed for the first time a novel tissue-engineered nerve guide conduit structure encapsulated with human endometrial stem cell (EnSC) derived exosomes, which promoted nerve regeneration in rat sciatic nerve defects. In this study, we initially indicated the long-term efficacy and safety impacts of newly designed double layered SF/PLLA nerve guide conduits. Then the regeneration effects of SF/PLLA nerve guide conduits containing exosomes derived from human EnSCs were evaluated in rat sciatic nerve defects. The human EnSC derived exosomes were isolated from the supernatant of human EnSC cultures and characterized. Subsequently, the human EnSC derived exosomes were encapsulated in constructed NGCs by fibrin gel. For in vivo studies, entire 10 mm peripheral nerve defects were generated in rat sciatic nerves and restored with NGC encapsulated with human EnSC derived exosomes (Exo-NGC group), nerve guide conduits, and autografts. The efficiency of the NGCs encapsulated with human EnSCs derived exosomes in assisting peripheral nerve regeneration was investigated and compared with other groups. The in vivo results demonstrated that encapsulated human EnSC derived exosomes in NGC (Exo-NGC) significantly benefitted nerve regeneration based on motor function, sensory reaction, and electrophysiological results. Furthermore, immunohistochemistry with histopathology results showed the formation of regenerated nerve fibers, along with blood vessels that newly were developed, as a result of the exosome functions in the Exo-NGC group. These outcomes illustrated that the newly designed core–shell SF/PLLA nerve guide conduit encapsulated with human EnSC derived exosomes enhanced the regeneration process of axons and improved the functional recovery of rat sciatic nerve defects. So, encapsulated human EnSC-derived exosomes in a core–shell SF/PLLA nerve guide conduit are a potential therapeutic cell-free treatment for peripheral nerve defects.

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“…Accordingly, animal studies have shown that various neurotrophic factors are expressed during nerve rupture 3,4 . As one of them, the glial cell line‐derived neurotrophic factor (GNDF) is up‐regulated after nerve injury, stimulates Schwann cell migration and promotes myelination 3,29–31 . It is conceivable that similar changes in neurotrophic factors occur after RLN injury 28,32–34 .…”

Section: Discussionmentioning

confidence: 99%

Artificial Nerve Conduit for Recurrent Laryngeal Nerve Reconstruction in Thyroid Surgery

et al. 2023

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Objective Oncological reconstruction of the recurrent laryngeal nerve (RLN) is sometimes necessary for RLN invaded by thyroid cancer. There have been no case reports of RLN reconstruction using artificial nerve conduits, which are often used for peripheral nerves. In this study, we retrospectively evaluate the feasibility, safety, and efficacy of a collagen conduit with collagen filaments for RLN reconstruction cases at our hospital. Methods Artificial nerve conduits were used in seven cases of RLN reconstruction. Two patients had preoperative unilateral vocal cord paralysis with severe vocal cord atrophy, and two had vocal cord paresis without atrophy. The remaining three patients had functional vocal cords before surgery that had to be resected via surgery due to thyroid cancer infiltration of the RLN. Reconstruction was performed using RENERVE®, which is a collagen conduit. Voice examination and laryngeal endoscopy were performed 1, 3, and 12 months after surgery. Results There was no improvement in the phonetics of the two patients with vocal cord atrophy before surgery. In the remaining five cases, three with functional vocal cords improved to preoperative values, and two with vocal cord paresis improved to greater than preoperative values. Conclusion We report the first case series using an artificial nerve conduit for human RLN reconstruction. In cases of RLN resection when the patient has good voice quality pre‐operatively, reconstruction of the RLN using an artificial nerve may be a favorable option in cases where direct anastomosis or ansa cervicalis to RLN anastomosis cannot be performed. Level of Evidence 4 Laryngoscope, 133:1773–1779, 2023

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Platelet-rich plasma loaded nerve guidance conduit as implantable biocompatible materials for recurrent laryngeal nerve regeneration

Cited by 12 publications

References 61 publications

Combined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAP

Combined laser-activated SVF and PRP remodeled spinal sclerosis via activation of Olig-2, MBP, and neurotrophic factors and inhibition of BAX and GFAP

Tissue-Engineered Core–Shell Silk-Fibroin/Poly-l-Lactic Acid Nerve Guidance Conduit Containing Encapsulated Exosomes of Human Endometrial Stem Cells Promotes Peripheral Nerve Regeneration

Artificial Nerve Conduit for Recurrent Laryngeal Nerve Reconstruction in Thyroid Surgery

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