Platelet-rich plasma improves expansion of human mesenchymal stem cells and retains differentiation capacity and<i>in vivo</i>bone formation in calcium phosphate ceramics

Vogel, J.; Szalay, Krisztián; Geiger, Florian; Kramer, Martin; Richter, Wiltrud; Kasten, Philip

doi:10.1080/09537100600758867

Cited by 165 publications

(126 citation statements)

References 15 publications

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“…However, the ''black box'' approach taken by many of the clinicians utilizing PRP leaves much to be done in the realm of basic science to fully understand and standardize the practice. To date, the collection of whole blood and the concentration and isolation of platelets to make PRP has been proven effective in vitro for stimulating cellular activity in a number of formats, both in liquid 13,[33][34][35]37,[39][40][41] and in lyophilized PRGF form. [16][17][18][19] To the best of the authors' knowledge, this article serves as the first instance of a powdered PRGF being incorporated into an electrospun tissue engineering scaffold to serve as a controlled release vehicle for such a concentrated growth factor and cytokine milieu.…”

Section: Discussionmentioning

confidence: 99%

“…This was expected, as it had previously been demonstrated that PRGF, as well as growth factors secreted by macrophages, enhanced fibroblast, mesenchymal, and stromal stem cell proliferation. 2,[33][34][35][36][37] In general, ADSC proliferation in all preconditioned medium increased from day 1 to day 4; however, by day 7 it appeared that proliferation slowed, and in some cases cell number even decreased, potentially due to induced contact inhibition as the cells became confluent in the wells, or died off following exhaustion of medium nutrients. This may also have been because the conditioned medium used for the ADSCs was MSFM, which is unfavorable over the long-term for ADSC growth, or due to harmful factors expressed during macrophage apoptosis.…”

Section: Released Prgf Effect On Cell Proliferationmentioning

confidence: 99%

“…These results suggest that the presence of PRGF does impact ADSC proliferation, and corroborates previously published work. 2,[33][34][35]37 It is clearly evident from these studies that the proliferation of ADSCs, cultured in conditioned medium, is different depending on the presence or absence of macrophages and macrophage-secreted factors over the 7-day study duration, and will be discussed further in the following section.…”

Section: Released Prgf Effect On Cell Proliferationmentioning

confidence: 99%

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Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Sell

Wolfe

Ericksen

et al. 2011

Tissue Engineering Part A

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Platelet-rich plasma (PRP) therapy has seen a recent spike in clinical interest due to the potential that the highly concentrated platelet solutions hold for stimulating tissue repair and regeneration. The aim of this study was to incorporate PRP into a number of electrospun materials to determine how growth factors are eluted from the structures, and what effect the presence of these factors has on enhancing electrospun scaffold bioactivity. PRP underwent a freeze-thaw-freeze process to lyse platelets, followed by lyophilization to create a powdered preparation rich in growth factors (PRGF), which was subsequently added to the electrospinning process. Release of protein from scaffolds over time was quantified, along with the quantification of human macrophage and adipose-derived stem cell (ADSC) chemotaxis and proliferation. Protein assays demonstrated a sustained release of protein from PRGF-containing scaffolds at up to 35 days in culture. Scaffold bioactivity was enhanced as ADSCs demonstrated increased proliferation in the presence of PRGF, whereas macrophages demonstrated increased chemotaxis to PRGF. In conclusion, the work performed in this study demonstrated that the incorporation of PRGF into electrospun structures has a significant positive influence on the bioactivity of the scaffolds, and may prove beneficial in a number of tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

Section: Released Prgf Effect On Cell Proliferationmentioning

confidence: 99%

Section: Released Prgf Effect On Cell Proliferationmentioning

confidence: 99%

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Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Sell

Wolfe

Ericksen

et al. 2011

Tissue Engineering Part A

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“…The local environment in the initial fracture hematoma is acidic, which later becomes more alkaline as the healing progress [1]. Platelet-rich plasma and platelet derivates contain several potent growth factors and have, therefore, been used for growth stimulation of a variety of mesenchymal cells [2][3][4][5]. Platelet preparations have also been used clinically to stimulate bone formation and wound healing [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Acidic preparations of lysed platelets upregulate proliferative pathways in osteoblast-like cells as demonstrated by genome-wide microarray analysis

Wahlström¹,

Linder²,

Ansell³

et al. 2011

Platelets

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latelets contain numerous growth factors essential for wound and fracture healing. We investigated the gene expression in human osteoblast-like cells stimulated with lysed platelets prepared in acidic, neutral, or alkaline buffers. Lysed platelets prepared in buffers at pH 5.4, 7.4, and 7.9, were added after neutralization to hFOB 1.19 cells. Genome-wide microarray analysis was performed using the Affymetrix GeneChip 7G Scanner. Biometric, cluster, and pathway analyses were performed with GeneSpring GX. Biometric analyses demonstrated that 53 genes were differentially regulated (p andlt;= 0.005, andgt;= 2-fold increase). Pathway analysis revealed 10 significant pathways of which eight are common ones regulating bone formation and cancer growth. Eleven genes were selected for quantitative real-time polymerase chain reaction (PCR) based on the microarray analysis of the lysed platelets prepared in the pH 5.4 experiments. In conclusion, acidic preparations of lysed platelet concentrates release factors essential for cell proliferation and particularly cell metabolism under hypoxic conditions. The genetic response from these factors was dominated by genes associated with the same pathways observed in bone formation and cancer growth. Activation of TGF-beta in the acidic preparation could be a stimulatory key factor of cell proliferation. These results support the hypothesis that acidification of platelets modifies the stimulatory response of mesenchymal cells in vitro, which is analogous with the observed milieu of a low pH present in wound and fracture sites, as well as in growing tumors.

Original Publication: Ola Wahlström, Cecilia Linder, Anna Ansell, Anders Kalén, Mats Söderström and Per Magnusson, Acidic preparations of lysed platelets upregulate proliferative pathways in osteoblast-like cells as demonstrated by genome-wide microarray analysis, 2011, Platelets, (22), 6, 452-460. http://dx.doi.org/10.3109/09537104.2011.565432 Copyright: Informa Healthcare http://informahealthcare.com/

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“…19 Cell density is a critical factor affecting the growth of cells. Culture attempts are usually unsuccessful below a critical cell density.…”

Section: Introductionmentioning

confidence: 99%

Potential role of mesenchymal stromal cells in pediatric hematopoietic SCT

Ball

Bernardo

Locatelli

et al. 2008

Bone Marrow Transplant

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Platelet-rich plasma improves expansion of human mesenchymal stem cells and retains differentiation capacity andin vivobone formation in calcium phosphate ceramics

Abstract: The replacement of FCS by PRP eliminated risks connected with the use of xenogeneic supplements. It improved expansion of MSC and retained their differentiation and in vivo bone formation capacity in a setting adaptable to autogenous use.

Cited by 165 publications

References 15 publications

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Acidic preparations of lysed platelets upregulate proliferative pathways in osteoblast-like cells as demonstrated by genome-wide microarray analysis

Potential role of mesenchymal stromal cells in pediatric hematopoietic SCT

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