Platelet Rich Plasma for Regenerative Medicine Treatment of Bovine Ovarian Hypofunction

Crémonesi, F.; Bonfanti, S; Idda, Antonella; Consiglio, A. Lange

doi:10.3389/fvets.2020.00517

Cited by 18 publications

(30 citation statements)

References 37 publications

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“…Similarly, platelet lysate induced a higher follicular survival with no effect follicle development during the in-vitro culture of isolated mouse secondary follicles [ 27 ]. In contrast, PRP improved ovarian function in cows with ovary hypofunction; this effect may be as a result of a reduction in follicular atresia or from revitalization of the dormant oocytes [ 28 ]. The positive effect of PRP or L-GF equina on follicular growth might be related to the presence of various growth factors that support the early stage of follicle development [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares

Dawod

Miró

Elbaz

et al. 2021

Animals

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This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasma (PRP) or equine lyophilized growth factor (L-GFequina) on the follicular growth, endometrial thickness, estrus cycle length, and pregnancy rate in purebred Arabian mares. A total of 73 purebred Arabian mares who experienced repeat breeding for three successive cycles were randomly divided into the following three groups: control group, without treatment; second Group (PRP group), in which mares were intrauterine infused with 20 mL of fresh PRP on the second day after the end of physic estrus phase; and the third group (L-GFequina Group), consisting of mares that were intrauterine infused with 20 mL of reconstituted lyophilized horse platelets growth factors (L-GFequina) on the second day after the estrus phase. The results showed no significant difference between control and treated groups in the diameter of the preovulatory follicles during the post treatment cycle. The endometrium thickness increased significantly in the L-GFequina and PRP groups ahead of the non-treated group. Intrauterine L-GFequina or PRP administration shortened the estrus cycle length. A higher pregnancy rate was recorded in the L-GFequina and PRP treated mares. In conclusion, the intrauterine infusion of L-GFequina or PRP increased the endometrial thickness and pregnancy rate and could be used to improve fertility in Arabian purebred mares who experienced from repeat breeding.

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Section: Discussionmentioning

confidence: 99%

Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares

Dawod

Miró

Elbaz

et al. 2021

Animals

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“…Treatment with PRP is a valuable therapy for improving the embryo recovery in superovulating cows [ 14 ]. Furthermore, it has been used to increase fertility in cows with ovarian hypofunction [ 15 ] and to increase the pregnancy rate in repeat breeding of pure Arabian mares [ 16 ]. Platelet-rich plasma could modulate the inflammatory and immune activation processes and potentially minimize inflammation-associated tissue damage [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GFequina) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows

Silviu‐Ionuț¹,

Dascălu²,

Borş

et al. 2022

Animals

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The purpose of this study was to determine the impact of intraovarian injections of a reconstituted lyophilized growth-promoting factor extracted from horse blood platelets (L-GFequina) on the number of ovarian follicles, the recovery of cumulus–oocyte complexes (COCs), and embryo development to the blastocyst stage in Holstein cows. Thus, 12 Holstein cows were assigned to three protocols. According to the number of punctured follicles in protocol 1, ovum pick-up (OPU) was conducted on days 6 and 14 of the cycle (day 0 = estrus). In protocol 2, every large follicle (more than 7 mm) was removed, and 1 mL of L-GFequina was intraovarian injected (day 0). Two days later, equine chorionic gonadotropin (eCG) was administered, and OPU sessions were conducted on days 6, 10, and 14. The same ovarian stimulation procedure as that in protocol 2 was performed in protocol 3, except that equine L-GFequina was not supplied. OPU was carried out on days 6 and 10 of the cycle. The results indicate that the intraovarian injection of L-GFequina significantly (p < 0.05) increased the number of OPU sessions per cycle, the recovery of cumulus–oocyte complexes (COCs), and the production of blastocysts. In conclusion, an intraovarian injection of L-GFequina can improves OPU-IVEP results in Holstein cows.

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“…After ovulation, this follicle will form the corpus luteum responsible for the secretion of progesterone. If a follicle of at least 8–15 mm is present on two consecutive examinations during the postpartum period and there is absence of cysts or luteal structures, it can be assumed that the cow may be affected by ovarian hypofunction and to have ovarian anovulation ( Cremonesi et al, 2020 ). These animals are healthy but sub-fertile thus causing considerable economic damage to farms.…”

Section: Ovarian Diseasesmentioning

confidence: 99%

Application of Perinatal Derivatives in Ovarian Diseases

Consiglio

Capra

Herrera

et al. 2022

Front. Bioeng. Biotechnol.

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Reproductive diseases could lead to infertility and have implications for overall health, most importantly due to psychological, medical and socio-economic consequences for individuals and society. Furthermore, economical losses also occur in animal husbandry. In both human and veterinary medicine, hormonal and surgical treatments, as well as assisted reproductive technologies are used to cure reproductive disorders, however they do not improve fertility. With ovarian disorders being the main reproductive pathology in human and bovine, over the past 2 decades research has approached regenerative medicine in animal model to restore normal function. Ovarian pathologies are characterized by granulosa cell and oocyte apoptosis, follicular atresia, decrease in oocyte quality and embryonic development potential, oxidative stress and mitochondrial abnormalities, ultimately leading to a decrease in fertility. At current, application of mesenchymal stromal cells or derivatives thereof represents a valid strategy for regenerative purposes. Considering their paracrine/autocrine mode of actions that are able to regenerate injured tissues, trophic support, preventing apoptosis and fibrosis, promoting angiogenesis, stimulating the function and differentiation of endogenous stem cells and even reducing the immune response, are all important players in their future therapeutic success. Nevertheless, obtaining mesenchymal stromal cells (MSC) from adult tissues requires invasive procedures and implicates decreased cell proliferation and a reduced differentiation capacity with age. Alternatively, the use of embryonic stem cells as source of cellular therapeutic encountered several ethical concerns, as well as the risk of teratoma formation. Therefore, several studies have recently focussed on perinatal derivatives (PnD) that can be collected non-invasively and, most importantly, display similar characteristics in terms of regenerating-inducing properties, immune-modulating properties and hypo-immunogenicity. This review will provide an overview of the current knowledge and future perspectives of PnD application in the treatment of ovarian hypofunction.

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Platelet Rich Plasma for Regenerative Medicine Treatment of Bovine Ovarian Hypofunction

Cited by 18 publications

References 37 publications

Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares

Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares

Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GFequina) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows

Application of Perinatal Derivatives in Ovarian Diseases

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