Platelet‐rich plasma for androgenic alopecia: A randomized, placebo‐controlled, double‐blind study and combined mice model experiment

Qu, Qian; Zhou, Yi; Shi, Panli; Du, Lijuan; Fan, Zhexiang; Wang, Jin; Li, Xin; Chen, Jian; Zhu, De-Cong; Yao, Ke; Hu, Zhiqi; Miao, Yong

doi:10.1111/jocd.14089

Cited by 17 publications

(19 citation statements)

References 38 publications

(63 reference statements)

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“…Meanwhile, a significant link exists between TNF-α antagonist exposure and the development of alopecia (Béné et al, 2014) and EGFR-RAS-RAF signaling in epidermal stem cells is inextricably linked to the beginning and end of the hair cycle (Doma et al, 2013). Moreover, DPC proliferation is promoted by VEGF through the VEGFR-2/ERK pathway (Qu et al, 2021). Therefore, the pathways observed to be enriched in the current study may be related to the effect of DPCs on epidermal composition under DHT treatment.…”

Section: Discussionmentioning

confidence: 75%

“…From these results, the most widely studied pathway related to AGA is the Wnt signaling pathway, which involves functional crosstalk between AR and Wnt signaling pathways in target tissues (Van Mater et al, 2003;Lo Celso et al, 2004;Kitagawa et al, 2009). Hair follicle regeneration is reportedly related to upregulation of the Akt pathway, which is induced by HFSC proliferation (Kim et al, 2019) and downregulation of the p53 pathway (Qu et al, 2021). Activated platelet-rich plasma supernatant also increases the quantity of DPCs by activating the MAPK and Akt signaling pathways (Xiao et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth

Zhang

Huang

et al. 2021

Front. Cell Dev. Biol.

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Dermal papillae are a target of androgen action in patients with androgenic alopecia, where androgen acts on the epidermis of hair follicles in a paracrine manner. To mimic the complexity of the dermal papilla microenvironment, a better culture model of human dermal papilla cells (DPCs) is needed. Therefore, we evaluated the inhibitory effect of dihydrotestosterone (DHT)-treated two-dimensional (2D)- and 3D-cultured DPCs on hair follicle growth. 2D- and 3D-cultured DPC proliferation was inhibited after co-culturing with outer root sheath (ORS) cells under DHT treatment. Moreover, gene expression levels of β-catenin and neural cell adhesion molecules were significantly decreased and those of cleaved caspase-3 significantly increased in 2D- and 3D-cultured DPCs with increasing DHT concentrations. ORS cell proliferation also significantly increased after co-culturing in the control-3D model compared with the control-2D model. Ki67 downregulation and cleaved caspase-3 upregulation in DHT-treated 2D and 3D groups significantly inhibited ORS cell proliferation. Sequencing showed an increase in the expression of genes related to extracellular matrix synthesis in the 3D model group. Additionally, the top 10 hub genes were identified, and the expression of nine chemokine-related genes in DHT-treated DPCs was found to be significantly increased. We also identified the interactions between transcription factor (TF) genes and microRNAs (miRNAs) with hub genes and the TF–miRNA coregulatory network. Overall, the findings indicate that 3D-cultured DPCs are more representative of in vivo conditions than 2D-cultured DPCs and contribute to our understanding of the molecular mechanisms underlying androgen-induced alopecia.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth

Zhang

Huang

et al. 2021

Front. Cell Dev. Biol.

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“…PRP promoting hair growth by activating WNT/β-Catenin signaling lead to proliferation and differentiation of hair follicle cells and triggering new hair cycle. 42 …”

Section: Use Of Platelet Rich Plasma In Hair Disordersmentioning

confidence: 99%

Platelet Rich Plasma and Its Use in Hair Regrowth: A Review

Paichitrojjana¹,

Paichitrojjana²

2022

DDDT

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Platelet rich plasma (PRP) was described as a small volume of plasma containing higher concentrations of platelets than those found in peripheral blood and initially used as a transfusion product for treatment of thrombocytopenia. To date, it was discovered that there are several growth factors and cytokines that can accelerate wound healing and tissue regeneration, leading to a wider range of applications in the medical field, such as in sport medicine, regenerative medicine, and aesthetic medicine. Several studies have shown that PRP can be used effectively for treatment of hair loss. Although it has been widely used, the exact mechanism of action of PRP is still not fully elucidated. In this article, we aim to review and update current information on the definition, classification, mechanism of action, clinical efficacy in hair regrowth, and adverse events of PRP.

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“…A recent randomized, placebo-controlled, half-head, double blind study in 2021 by Qu et al further substantiated the clinical efficacy of PRP in the treatment of AGA ( 98 ). 32 male patients with stage II-V AGA (Norwood-Hamilton scale) and 20 female AGA patients with stage I-III AGA (Ludwig scale) were subdermally injected with PRP on one half-head of the affected scalp area while the other half-head were injected with saline solution.…”

Section: Overview Of Recent Clinical Trials Using Prp For the Treatme...mentioning

confidence: 95%

Treatment of Androgenetic Alopecia Using PRP to Target Dysregulated Mechanisms and Pathways

2022

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Androgenetic alopecia (“AGA”) is the most prevalent type of progressive hair loss, causing tremendous psychological and social stress in patients. However, AGA treatment remains limited in scope. The pathogenesis of androgenetic alopecia is not completely understood but is known to involve a hair follicle miniaturization process in which terminal hair is transformed into thinner, softer vellus-like hair. This process is related to the dysregulation of the Wnt/β-catenin signaling pathway, which causes premature termination of the anagen growth phase in hair follicles. Historically used for wound healing, platelet rich plasma (“PRP”) has recently been at the forefront of potential AGA treatment. PRP is an autologous preparation of plasma that contains a high number of platelets and their associated growth factors such as EGF, IGF-1, and VEGF. These factors are known to individually play important roles in regulating hair follicle growth. However, the clinical effectiveness of PRP is often difficult to characterize and summarize as there are wide variabilities in the PRP preparation and administration protocols with no consensus on which protocol provides the best results. This study follows the previous review from our group in 2018 by Cervantes et al. to analyze and discuss recent clinical trials using PRP for the treatment of AGA. In contrast to our previous publication, we include recent clinical trials that assessed PRP in combination or in direct comparison with standard of care procedures for AGA such as topical minoxidil and/or oral finasteride. Overall, this study aims to provide an in-depth analysis of PRP in the treatment of AGA based on the evaluation of 17 recent clinical trials published between 2018 and October 2021. By closely examining the methodologies of each clinical trial included in our study, we additionally aim to provide an overall consensus on how PRP can be best utilized for the treatment of AGA.

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Platelet‐rich plasma for androgenic alopecia: A randomized, placebo‐controlled, double‐blind study and combined mice model experiment

Cited by 17 publications

References 38 publications

Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth

Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth

Platelet Rich Plasma and Its Use in Hair Regrowth: A Review

Treatment of Androgenetic Alopecia Using PRP to Target Dysregulated Mechanisms and Pathways

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