Platelet-rich plasma-based bioactive membrane as a new advanced wound care tool

Spanò, Raffaele; Muraglia, Anita; Todeschi, Maria Rosa; Nardini, Marta; Strada, Paolo; Cancedda, Ranieri; Mastrogiacomo, Maddalena

doi:10.1002/term.2357

Cited by 20 publications

(18 citation statements)

References 55 publications

(106 reference statements)

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“…In this study, capturing of the PRP-released growth factors by the heparin-conjugated fibrin guaranteed a sustained release of growth factors [54]. Recently, it was reported that membranes generated by the combination of PRP, cryoprecipitate prepared from platelet-poor plasma, and thrombin can serve as a bioactive adhesive, which was shown to mediate wound repair even in a challenged mouse model of chronic ulcers [55].…”

Section: Prp In Tissue-engineered Constructsmentioning

confidence: 99%

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Lang

Loibl²,

Herrmann³

2018

Eur Surg Res

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Background: Platelet-rich plasma (PRP) refers to an enriched platelet suspension in plasma. In addition to the clinical application of PRP in the context of various orthopedic diseases and beyond, PRP and platelet lysate (PL) have been in focus in the field of tissue engineering. In this review, we discuss the application of PRP as a cell culture supplement and as part of tissue engineering strategies, particularly emphasizing current hurdles and ambiguities regarding the efficacy of PRP in these approaches. Summary: As a putative autologous replacement for animal-derived supplements such as fetal calf serum (FCS), PRP has been applied as cell culture supplement for the expansion of stem and progenitor cells for tissue engineering applications and cell therapies. Attributed to the high content of growth factors in platelets, PRP has been shown to promote cell growth, which was mostly superior to standard cultures supplemented with FCS, while the differentiation capacity of progenitor cells seems not to be affected. However, it was also suggested that cultivation of cells with PRP significantly alters the protein expression profile in cells in comparison to FCS, indicating that the influence of PRP on cell behavior should be thoroughly investigated. Moreover, different PRP preparation methods and donor variations have to be considered for the use of PRP under good manufacturing practice conditions. PRP has been used for various tissue engineering applications in the context of bone, cartilage, skin, and soft tissue repair, where most studies were conducted in the field of bone tissue engineering. These approaches take either advantage of the release of chemoattractive, angiogenic, proliferative, and putatively pro-regenerative growth factors from PRP, and/or the hydrogel properties of activated PRP, making it suitable as a cell delivery vehicle. In many of these studies, PRP is combined with biomaterials, cells, and in some cases recombinant growth factors. Although the experimental design often does not allow conclusions on the pro-regenerative effect of PRP itself, most publications report beneficial effects if PRP is added to the tissue-engineered construct. Furthermore, it was demonstrated that the release of growth factors from PRP may be tailored and controlled when PRP is combined with materials able to capture growth factors. Key Messages: Platelet-derived preparations such as PRP and PL represent a promising source of autologous growth factors, which may be applied as cell culture supplement or to promote regeneration in tissue-engineered constructs. Furthermore, activated PRP is a promising candidate as an autologous cell carrier. However, the studies investigating PRP in these contexts often show conflicting results, which most likely can be attributed to the lack of standardized preparation methods, particularly with regard to the platelet content and donor variation of PRP. Ultimately, the use of PRP has to be tailored for the individual application.

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Section: Prp In Tissue-engineered Constructsmentioning

confidence: 99%

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Lang

Loibl²,

Herrmann³

2018

Eur Surg Res

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“…Considering the controversial literature data [40][41][42][43] on the effectiveness of the PRP, we figured that the inconsistency of the published results was most probably due to a poor quality of PRP produced by inadequate and uncontrolled procedures and to the extreme variability of the PRP product prepared from a single blood donation without considering the significant inter-individual variability in the platelet concentration of human plasma and the variability within the same individual, according to his health status. Therefore, the PL used for the biomembrane production was derived from large pools of blood donations collected according to blood banking safety practices and processed following standardized industrialized procedures [6,7,44]. Our preliminary clinical results have shown that the PRP, prepared according to controlled procedures, has a beneficial effect in the treatment of chronic skin ulcers (data not shown).…”

Section: Discussionmentioning

confidence: 97%

“…The limits of this approach were attributed to the lack of standardized procedures in the platelet derivatives protocols and interindividual variability of blood donation. Part of this has found an answer by its characterization in terms of platelet concentration and contained growth factors [6,7].…”

Section: Introductionmentioning

confidence: 99%

Growth Factors Delivery System for Skin Regeneration: An Advanced Wound Dressing

et al. 2020

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Standard treatments of chronic skin ulcers based on the direct application of dressings still present several limits with regard to a complete tissue regeneration. Innovative strategies in tissue engineering offer materials that can tune cell behavior and promote growth tissue favoring cell recruitment in the early stages of wound healing. A combination of Alginate (Alg), Sericin (SS) with Platelet Lysate (PL), as a freeze-dried sponge, is proposed to generate a bioactive wound dressing to care skin lesions. Biomembranes at different composition were tested for the release of platelet growth factors, cytotoxicity, protective effects against oxidative stress and cell proliferation induction. The highest level of the growth factors release occurred within 48 h, an optimized time to burst a healing process in vivo; the presence of SS differently modulated the release of the factors by interaction with the proteins composing the biomembranes. Any cytotoxicity was registered, whereas a capability to protect cells against oxidative stress and induce proliferation was observed when PL was included in the biomembrane. In a mouse skin lesion model, the biomembranes with PL promoted the healing process, inducing an accelerated and more pronounced burst of inflammation, formation of granulation tissue and new collagen deposition, leading to a more rapid skin regeneration.

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“…“Platelet concentrates” (PCs) is a generic term for different products enriched in platelets . Over the past decade, PCs have been used widely in the regeneration and repair of tissue for wound healing, bone fractures, osteoarthritis, skin rejuvenation, and hair growth . However, their application is confusing and the classification of terminologies for PCs is lacking.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, autologous PRP derivatives have been used widely in dermatology, orthopedics, sports medicine, oral/maxillofacial surgery, cardiac surgery, gynecology, ophthalmology, cosmetic surgery, plastic, and reconstructive surgery . However, several studies have reported that PRP has no proven effect .…”

mentioning

confidence: 99%

Application of standardized platelet‐rich plasma in elderly patients with complex wounds

Tian

Cheng

Chen

et al. 2019

Wound Repair Regeneration

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In recent years, autologous platelet-rich plasma (PRP) derivatives have been used widely in the regeneration and repair of tissue, but a standard definition and preparation method for PRP are lacking. We developed a standardized method using platelet indices as quality-control indicators for PRP preparation. Twenty-one elderly patients (9 males, 12 females) with complex wounds were treated with standardized platelet-rich plasma (S-PRP). The platelet count in PRP after the second centrifugation was 1,069-1,436 × 10 9 /L. We adjusted the platelet concentration in PRP after a second centrifugation to 1,000 × 10 9 /L according to a formula using platelet-poor plasma (PPP). The standardized preparation method that we developed gave S-PRP with a relatively uniform platelet concentration. The wounds of 21 patients showed accelerated healing after S-PRP treatment, and there were no obvious side effects during treatment. These data suggest that our preparation method of S-PRP, using platelet indices as quality-control indicators with platelet count of 1,000 × 10 9 /L could be used for the treatment of complex wounds in the elderly. The preparation method of S-PRP proposed in the present study may be a simple and effective method of PRP quality control.

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Platelet-rich plasma-based bioactive membrane as a new advanced wound care tool

Cited by 20 publications

References 55 publications

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Growth Factors Delivery System for Skin Regeneration: An Advanced Wound Dressing

Application of standardized platelet‐rich plasma in elderly patients with complex wounds

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