Platelet rich plasma and dentine effect on sheep dental pulp cells regeneration/revitalization ability (<i>in vitro</i>)

Altaii, Milad; Kaidonis, Xenia; Koblar, Simon; Cathro, Peter; Richards, Lindsay

doi:10.1111/adj.12426

Cited by 11 publications

(10 citation statements)

References 33 publications

(44 reference statements)

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“…A few studies pointed out that PRP could improve odontogenic/osteogenic differentiation [13,34,35] and mineralization [11,12] of dental stem cells. PRP promoted mRNA and protein expression of DSPP and DMP-1 in SCAP [13].…”

Section: Discussionmentioning

confidence: 99%

“…PRP is a favorable autologous scaffold that can facilitate cell proliferation and differentiation [10]. Dental pulp stem cells (DPSCs) cultured with a certain concentration of PRP exhibited preferable proliferation, migration, and mineralization [11,12]. SCAP cultured with PRP expressed a high level of odontogenic markers such as dentin matrix protein 1 (DMP-1) and dentin sialophosphoprotein (DSPP) [13].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Platelet-Rich Plasma on Proliferation, Viability, and Odontogenic Differentiation of Neural Crest Stem-Like Cells Derived from Human Dental Apical Papilla

Xiang

Guan

et al. 2020

BioMed Research International

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Objective. This study is aimed at evaluating the effects of platelet-rich plasma (PRP) on proliferation, viability, and odontogenic differentiation of neural crest stem-like cells (NCSCs) derived from human dental apical papilla. Materials and Methods. Cells from apical papillae were obtained and then induced to form neural spheres. The expression of NCSC markers p75NTR and HNK-1 in neural sphere cells was detected by immunofluorescence staining. Human PRP was prepared by a 2-step centrifugation method and activated by CaCl2 and thrombin. The concentrations of PDGF-BB and TGF-β1 in whole blood and PRP were measured by an ELISA kit. PRP in five different concentrations (0%, 2.5%, 5%, 10%, and 25%) was applied to culture NCSCs. On the 1st, 3rd, 5th, and 7th days, cell proliferation was evaluated by CCK8. Cell viability was tested by a live/dead staining kit. mRNA and protein expression of DSPP and BMP4 were analyzed by RT-qPCR and western blot, respectively. Statistical analysis was performed by a one-way analysis of variance (ANOVA) test or t-test. Results. Dental apical papilla cells formed neural spheres, from which cells displayed positive expression of p75NTR and HNK-1. The concentrations of PDGF-BB and TGF-β1 in PRP were about 3.5-fold higher than those in whole blood. 5% and 10% PRP significantly promoted proliferation of NCSCs, while 25% and 50% PRP inhibited cell proliferation from Day 3 to Day 7. Low-concentration (2.5%, 5%, and 10%) PRP slightly improved viability of NCSCs on Day 7. On the other hand, high-concentration (25% and 50%) PRP significantly inhibited viability of NCSCs from Day 3 to Day 7. RT-qPCR and western blot results indicated that 10% PRP could promote odontogenic differentiation of NCSCs on Day 7. mRNA and protein expression of DSPP and BMP4 were significantly upregulated in the 10% PRP group compared to those in the control group (P<0.05). Conclusions. PRP is a simply acquirable blood derivative which contains high concentration of growth factors like PDGF-BB and TGF-β1. PRP in a proper concentration could promote proliferation, viability, and odontogenic differentiation of NCSCs derived from human dental apical papilla.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Platelet-Rich Plasma on Proliferation, Viability, and Odontogenic Differentiation of Neural Crest Stem-Like Cells Derived from Human Dental Apical Papilla

Xiang

Guan

et al. 2020

BioMed Research International

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“…A systematic review of 63 articles published between 1922 and 2002 alarmingly concluded that success rates of primary root canal treatment have not improved over the past 5 decades, ranging between 68% and 85% (Ng et al 2007). Therefore, current efforts are focused on different approaches to endodontic treatment, for example, revitalization of dental pulp to restore health inside the canal and promote the resolution of apical infection (Prescott et al 2008; Sun et al 2011; Huang and Garcia-Godoy 2014; Del Fabbro et al 2016; Altaii et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Cell-Based Regenerative Endodontics for Treatment of Periapical Lesions: A Randomized, Controlled Phase I/II Clinical Trial

et al. 2020

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A randomized controlled phase I/II clinical trial was designed to evaluate the safety and efficacy of encapsulated human umbilical cord mesenchymal stem cells in a plasma-derived biomaterial for regenerative endodontic procedures (REPs) in mature permanent teeth with apical lesions. The trial included 36 patients with mature incisors, canines, or mandibular premolars showing pulp necrosis and apical periodontitis. Patients were randomly and equally allocated between experimental (REP) or conventional root canal treatment (ENDO) groups. On the first visit, cavity access and mechanical preparation of the root canal were performed. Calcium hydroxide medication was used, and the cavity was sealed. Three weeks later, patients were treated following their assigned protocol of ENDO or REP. Clinical follow-up examinations were performed at 6 and 12 mo. Categorical variables were evaluated by Fisher’s exact test. Quantitative variables were compared using the Mann-Whitney test. The evolution over time of the percentage of perfusion units and the dimensions of lesion and cortical compromise were explored. After the 12-mo follow-up, no adverse events were reported, and the patients showed 100% clinical efficacy in both groups. Interestingly, in the REP group, the perfusion unit percentage measured by laser Doppler flowmetry revealed an increase from 60.6% to 78.1% between baseline and 12-mo follow-up. Sensitivity tests revealed an increase of the positive pulp response in the REP group at 12-mo follow-up (from 6% to 56% on the cold test, from 0% to 28% on the hot test, and from 17% to 50% on the electrical test). We present the first clinical safety and efficacy evidence of the endodontic use of allogenic umbilical cord mesenchymal stem cells encapsulated in a plasma-derived biomaterial. The innovative approach, based on biological principles that promote dentin-pulp regeneration, presents a promising alternative for the treatment of periapical pathology (ClinicalTrials.gov NCT03102879).

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“…29,30 While BC has been used traditionally as a scaffold in regenerative endodontic procedures, 31 alternatives such as scaffold with stem cells or growth factors have recently emerged as possible tools to enhance regeneration treatment outcomes. [32][33][34] It is worth noting that the use of alternative scaffolds or the addition of cells or materials to existing scaffolds may increase the required treatment sessions, the complexity of treatment, and the cost, plus it can potentially cause infection. Therefore, to justify these changes to the original scaffold, it is important to identify their effects on the tissues generated on the root walls and in the root canal space after treatment.…”

mentioning

confidence: 99%

Histological assessment of regenerative endodontic treatment in animal studies with different scaffolds: A systematic review

Altaii

Richards

Rossi‐Fedele

2017

Dental Traumatology

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Platelet rich plasma and dentine effect on sheep dental pulp cells regeneration/revitalization ability (in vitro)

Abstract: A PRP scaffold has a positive effect on the proliferation, migration and differentiation of ODPC; however, dentine discs have an adverse effect on the activity of ODPC.

Cited by 11 publications

References 33 publications

Effects of Platelet-Rich Plasma on Proliferation, Viability, and Odontogenic Differentiation of Neural Crest Stem-Like Cells Derived from Human Dental Apical Papilla

Effects of Platelet-Rich Plasma on Proliferation, Viability, and Odontogenic Differentiation of Neural Crest Stem-Like Cells Derived from Human Dental Apical Papilla

Cell-Based Regenerative Endodontics for Treatment of Periapical Lesions: A Randomized, Controlled Phase I/II Clinical Trial

Histological assessment of regenerative endodontic treatment in animal studies with different scaffolds: A systematic review

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