Platelet-Rich Fibrin Lysate Can Ameliorate Dysfunction of Chronically UVA-Irradiated Human Dermal Fibroblasts

Abstract

To determine whether platelet-rich fibrin lysate (PRF-L) could restore the function of chronically ultraviolet-A (UVA)-irradiated human dermal fibroblasts (HDFs), we isolated and sub-cultured HDFs from six different human foreskins. HDFs were divided into two groups: those that received chronic UVA irradiation (total dosages of 10 J cm-2) and those that were not irradiated. We compared the proliferation rates, collagen deposition, and migration rates between the groups and between chronically UVA-irradiated HDFs in control and PRF-L-treated media. Our experiment showed that chronic UVA irradiation significantly decreased (p<0.05) the proliferation rates, migration rates, and collagen deposition of HDFs, compared to controls. Compared to control media, chronically UVA-irradiated HDFs in 50% PRF-L had significantly increased proliferation rates, migration rates, and collagen deposition (p<0.05), and the migration rates and collagen deposition of chronically UVA-irradiated HDFs in 50% PRF-L were equal to those of normal fibroblasts. Based on this experiment, we concluded that PRF-L is a good candidate material for treating UVA-induced photoaging of skin, although the best method for its clinical application remains to be determined.

Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies

“…Proliferation PRF increased proliferation of mesenchymal cells, for example from bone of different origin [19, 24-26, 28, 45, 50, 66, ], bone marrow [32,39], periosteum [27], adipose tissue [37,47,68], and skin [65,48]. Also, fibroblasts from gingiva [38,44], periodontal ligament [18,52,59], papilla [30], and dental pulp responded to PRF with increased proliferation [29,31,43,54].…”

“…There are various methods to identify the impact of PRF on cell migration including the scratch assay [70] and the traditional Boyden chamber approach [71]. Regardless of the method used, PRF increased the migration of neural stem cells [54] along with cells of the mesenchymal lineage isolated from bone [45,64], bone marrow [72], gingiva [38,64,36], apical papilla [30], and skin [65,48]. Similarly, endothelial cells responded to PRF with an increased migration [63,72,41].…”

Effects of platelet-rich plasma and platelet-poor plasma on human dermal fibroblasts

“…Although the challenged fibroblasts proliferation rates are less than the unchallenged fibroblasts, survival and proliferation are nonetheless partially restored. The authors proposed that the fibroblasts restoration was due to the MMP-1 tissue inhibitors released from the PRP and TGF-1 signalling ameliorated suppressed proliferation [54].…”

The Use of Platelet-Rich Fibrin in Bone Grafting

“…It is said to be a good source of several growth factors. In a study, it is found that PRF-L can significantly improve the proliferation index, collagen deposition and migration rates in chronically UVA-irradiated human dermal fibroblasts [13]. This is a new technique that requires further studies.…”