Platelet-Rich Fibrin Decreases the Inflammatory Response of Mesenchymal Cells

Kargarpour, Zahra; Nasirzade, Jila; Panahipour, Layla; Miron, Richard J.; Gruber, Reinhard

doi:10.3390/ijms222111333

Cited by 25 publications

(22 citation statements)

References 55 publications

(78 reference statements)

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“…In this setting, the desalted lysates of both allografts had an anti-inflammatory activity with respect to the expression of IL1 and IL6 in the RAW 264.7 macrophages. To further rule out this possibility, we implemented the ST2 murine mesenchymal cell line where IL1β and TNFα can provoke an inflammatory response [19]. We show here that with ST2 cells, desalted DGC and MDF significantly attenuated the expression of IL6, iNOS and CCL5.…”

Section: Discussionmentioning

confidence: 90%

“…We then applied the lysates of DGC and MDF to our established in vitro inflammation assay. Our bioassays include murine RAW 264.7 macrophages and primary bone marrow-derived macrophages being exposed to bacterial endotoxins [18], as well as murine ST2 bone marrow-derived mesenchymal cells [19] and human gingival fibroblasts being exposed to inflammatory cytokines interleukin 1β and TNFα [20]. The modulation of the inflammatory response is measured by changes in gene expression, including IL6, and by the intensity and nuclear translocation of phosphorylated p65 [21].…”

Section: Introductionmentioning

confidence: 99%

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Anti-Inflammatory Activity of a Demineralized Bone Matrix: An In Vitro Pilot Study

et al. 2022

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Demineralized bone matrix (DBM) is commonly used for the reconstruction of bone defects. Early graft consolidation involves a transient inflammatory process. It is, however, unclear whether DBM can modulate this process. To test this possibility, we prepared acid lysates of demineralized ground cortical (DGC) and moldable demineralized fibers (MDF). Murine RAW 264.7 and primary bone marrow macrophages were exposed to acid lysates of DGC and MFD prior to provoking an inflammatory response with lipopolysaccharide (LPS). Similarly, murine ST2 mesenchymal cells were exposed to DGC and MFD with and without interleukin 1β (IL1) and TNFα. We show here that acid lysates of DGC and MFD reduced the expression of IL1 and IL6 in RAW 264.7 macrophages, as determined by RT-PCR and, for IL6, by immunoassay. This response was confirmed with primary macrophages. Likewise, desalted acid lysates exert anti-inflammatory properties on RAW 264.7 cells and in ST2 cells, the forced expression of IL6, inducible nitric oxide synthase (iNOS) and chemokine ligand 5 (CCL5) was reduced. These in vitro findings suggest that DGC and MFD lower the inflammation-induced expression of inflammatory mediators in murine cell-based bioassays.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Activity of a Demineralized Bone Matrix: An In Vitro Pilot Study

et al. 2022

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“…Furthermore, as macrophages appear to undergo proliferation in vivo with estrogen exposure, 20 the paucity of this indispensable female hormone seen with advanced age gradually dims another promoter of macrophage action. Clinical trial data suggest this downward estrogen spiral can be rewound with intraovarian PRP, 21 thus replenishment of the ovarian macrophage pool and blunting of inflammatory signaling may be a secondary effect of this approach 22 …”

Section: Recent Progressmentioning

confidence: 99%

Ovarian recovery via autologous platelet‐rich plasma: New benchmarks for condensed cytokine applications to reverse reproductive aging

Sills

2022

Aging Medicine

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Health and life expectancy gains have pushed the overall number of menopausal patients to record levels. Because maternal age at first pregnancy also continues to rise, it is unsurprising that reduced birth rates are consistently reported across many populations. Both trends severely strain national demographics and present a socioeconomic challenge for which no satisfactory solution currently exists. Symptomatic menopause and infertility/miscarriage are met with standard therapies like hormone replacement therapy (HRT) and in vitro fertilization, respectively. Although these accepted interventions do supply some cover, both are expensive, low yield, and not without controversy. Meanwhile, ovarian steroid output and competent oocyte availability approach unrecoverable loss beyond age ~35 years, irrespective of treatment. Received wisdom holds that postnatal oogenesis in humans is impossible, a tenet which until recently encountered little serious confrontation. Reassessing this paradigm is overdue given proof‐of‐concept work on native sex steroid rejuvenation, de novo euploid oogenesis, ovulation, blastocyst development, fetal growth, and healthy term livebirths—all apparently possible with intraovarian insertion of platelet‐rich plasma (PRP). Discrete functional analysis of the full platelet‐derived cytokine array carried with PRP unfortunately for now, is incomplete. Here, selected platelet releasate constituents and measured effects are framed to address advances in wellness and women’s health. Emphasis is on cytokines best positioned to enable recovery of senescent ovarian function sufficient to suspend synthetic HRT dependency and/or permit egg retrieval and pregnancy. Whereas the chronicle of progress in other clinical fields does invite generalization of fresh platelet applications to reproductive endocrinology, basic mechanistic questions remain open.

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“…Accordingly, due to their enriched cargo in bioactive compounds, including growth factors and anti-inflammatory molecules, platelet preparation lysates represent a successful strategy for treatment of inflammatory conditions and regenerative medicine. By investigating the in vitro anti-inflammatory activity of various platelet-rich fibrin preparations (PRFs), Kargarpour and colleagues [ 6 ] highlight the species-specificity, as they show that PRFs exert potent anti-inflammatory activity on murine, but not on human, mesenchymal cells. As shown by Sovkova and co-workers [ 7 ], preparation protocols also contribute to variability of PRF effects; by comparison of human plasma and/or platelet lysates produced by different methods, they elegantly demonstrate that platelet proteins alone are not sufficient for providing optimal growth and viability of murine fibroblasts and human mesenchymal stem cells.…”

Section: Original Articlesmentioning

confidence: 99%