Platelet-rich Concentrates Differentially Release Growth Factors and Induce Cell Migration In Vitro

Schär, Michael; Diaz-Romero, José; Kohl, Sandro; Zumstein, Matthias A.; Nešić, Dobrila

doi:10.1007/s11999-015-4192-2

Cited by 218 publications

(230 citation statements)

References 38 publications

Supporting

Mentioning

203

Contrasting

Unclassified

Order By: Relevance

“…PDGF and TGF-β1 are the majority growth factors and the most important in PRPs and many reports focus especially on PDGF, TGF-β1 and IGF [47][48][49][50]. In our experiment we observed a mean constant accumulation of PDGF-AB and TGF-β1.…”

Section: Discussionsupporting

confidence: 66%

“…In particular, PDGF-AB was linear up to 3 rd day, while VEGF was linear up to 6 th day when it reached its plateau. These data are supported by a recent paper where the growth factor release of L-PRF from 8 hours to 28 days was evaluated, showing a non-linear kinetic release [47]. As shown above, there are different results in the literature concerning the quantitative measurement of the growth factors contained in PRPs [48,49].…”

Section: Discussionsupporting

confidence: 64%

See 1 more Smart Citation

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Borsani¹,

Bonazza²,

Buffoli³

et al. 2015

Biol Med (Aligarh)

View full text Add to dashboard Cite

SummaryScientific background: Platelet concentrates are nowadays widely applied in different clinical fields to improve soft tissue and bone regeneration. "Concentrated Growth Factors" (CGF) is a new generation of platelet concentrate products, which exhibits an interesting clinical and biotechnological application potential. Aim of the study:The aim of this study is to assess the biological rationale for the use of CGF, by evaluating blood cell localization, the in vitro cumulative release of seven growth factors (PDGF-AB, VEGF, TNF-α, TGF-β1, BDNF, BMP-2 and IGF-1), its in vitro effects on cell proliferation and its mechanical behavior.Methods: CGFs were obtained from volunteer donors. Blood cell localization was evaluated after properly morphological staining and immunohistochemistry. The amount of growth factors release was measured at 5 hours, 1, 3, 6, 7 and 8 days, using ELISA assay. Cells were cultured with and without CGF and their proliferation was evaluated after 72 hours, performing the quantification of Ki-67, using flow cytometry (FACS). The mechanical response of CGF under compression was also attempted. Results:The results showed that platelets and leukocytes were found in a very thin space called "buffy coat", localized between the white and red part of CGF. Each growth factor evaluated, had a specific kinetic release with a great variability among subjects. The in vitro cell proliferation was stimulated. CGF showed an "apparent plasticity" and its mechanical response was influenced by fibrin network structure. Conclusion:These findings support the CGF's clinical use and will allow us to better understand and improve the clinical outcomes. B io lo g y and M e d ic in e

show abstract

Section: Discussionsupporting

confidence: 66%

Section: Discussionsupporting

confidence: 64%

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Borsani¹,

Bonazza²,

Buffoli³

et al. 2015

Biol Med (Aligarh)

View full text Add to dashboard Cite

show abstract

“…[6][7][8][9] Improved engineered vasculature would allow ischemic and traumatic tissue defects to be treated more effectively and efficiently. [9][10][11][12][13] The insulin super-family is composed of 10 members in mammals, insulin, 2 insulin-like growth factors, 3 relaxin proteins, and 4 insulinlike peptides.…”

Section: Vascular Endothelial Growth Factor (Vegf) a And Fibroblast mentioning

confidence: 99%

Enhancing engineered vascular networks in vitro and in vivo: The effects of IGF1 on vascular development and durability

et al. 2017

View full text Add to dashboard Cite

Objectives: Creation of functional, durable vasculature remains an important goal within the field of regenerative medicine. Engineered biological vasculature has the potential to restore or improve human tissue function. We hypothesized that the pleotropic effects of insulin-like growth factor 1 (IGF1) would enhance the engineering of capillary-like vasculature. Results: IGF1 supplementation significantly enhanced de novo vasculogenesis both in vitro and in vivo. Effects were long-term as they lasted the duration of the study period, and included network density, vessel length, and diameter. Bifurcation density was not affected. However, the highest concentrations of IGF1 tested were either ineffective or even deleterious. Sustained IGF1 delivery was required in vivo as the inclusion of recombinant IGF1 protein had minimal impact.Conclusion: IGF1 supplementation can be used to produce neovasculature with significantly enhanced network density and durability. Its use is a promising methodology for engineering de novo vasculature to support regeneration of functional tissue.

show abstract

“…In this context, platelets are able to express a series of biologically active signaling molecules and growth factors, such as platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF) and transforming growth factor beta (TGF-β). These growth factors are essential for tissue vascularization and new tissue formation [8,9]. Moreover, platelets contain granules with cytokines, chemokines and other inflammatory mediators that are released after platelet aggregation to enhance hemostasis and activate and recruit cells to the site of inflammation [10,11].…”

Section: Introductionmentioning

confidence: 99%

Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept)

Bagdadi

Kubesch

et al. 2017

Eur J Trauma Emerg Surg

128

129

View full text Add to dashboard Cite

Moreover, platelets were located homogenously throughout the matrix in the A-PRF and A-PRF+ groups, whereas platelets in PRF were primarily observed within the lower portion. Discussion the present results show an increase growthfactor release by decreased RCF. However, further studies must be conducted to examine the extent to which enhancing the amount and the rate of released growth factors influence wound healing and biomaterial-based tissue regeneration. Conclusion These outcomes accentuate the fact that with a reduction of RCF according to the previously LSCC (described low speed centrifugation concept), growth factor release can be increased in leukocytes and platelets within the solid PRF matrices.

show abstract

Platelet-rich Concentrates Differentially Release Growth Factors and Induce Cell Migration In Vitro

Abstract: By characterizing the biologic properties of different platelet concentrates in vitro, we may gain a better understanding of their clinical effects and develop guidelines for specific future applications.

Cited by 218 publications

References 38 publications

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Enhancing engineered vascular networks in vitro and in vivo: The effects of IGF1 on vascular development and durability

Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept)

Contact Info

Product

Resources

About